Production and Characterization of Monoclonal Antibodies to Bovine Skin Proteodermatan Sulfate

Immunohistochemical localization of proteoglycans in bovine skeletal muscle and adipose connective tissues

“…Pringle et al (1985) raised MAbs against bovine decorin, and Avnur and Geiger (1984) a MAb specific to chondroitin sulphate. This report describes our preliminary study to immunohistochemically localize proteoglycans in bovine muscle epimysium, perimysium, endomysium and adipose connective tissues using MAbs specific to decorin and chondroitin sulphate.…”

“…Sections were then immunolocalized with MAbs to decorin (6D6, Pringle et al 1985) and chondroitin sulphate (CS-56, Avnur and Geiger 1984, Sigma Chemical Co. St. Louis, MO). The MAb 6D6 recognizes an epitope in the Cterminal portion of the protein core (Scott et al 1993).…”

“…Final incubation was carried out using mouse peroxidase-anti-peroxidase complex (Nordic Immunology Tilberg, The Netherland) diluted 1 in 300. Color was developed with diaminobenzidine tetrahydrochloride (Sigma) and hydrogen peroxide (Pringle et al 1985).…”

A histochemical and immunohistochemical study of proteoglycans in normal and osteochondrotic joint cartilage of swine

“…) and 6D6 (Pringle et al 1985) which were raised against CS, KS and bovine decorin, respectively. The MAb 6D6 recognizes porcine decorin (Pringle et al 1985 (Fig.…”

Analysis of tissues from normal lambs and those with spider syndrome

“…Sections were also stained immunohistochemically using monoclonal antibodies raised against dermatan sulfate proteoglycan, decorin (Pringle et al 1985), chondroitin sulfate (CS-56, Sigma Chemical Co.), and keratan sulfate (Nakano et al 1993 tissues were also analyzed for calcium and phosphorus (Nakano et al 1982). A paired /-test was used to detect significant differences between means.…”