Production and characterization of monoclonal antibodies (mAbs) against human serum albumin (HSA) for the development of an immunoaffinity system with oriented anti-HSA mAbs as immobilized ligand

Rajak, Poonam; Vijayalakshmi, M.A.; Jayaprakash, N.S.

doi:10.1016/j.jpba.2013.02.010

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…The fused cells were aliquoted in 96‐well cell culture plates and placed in a humidified CO 2 (5%) incubator at 37 °. On 7th day, the media was changed and maintained until the growth of hybridoma was observed [16, 20, 21]. The hybridoma grown with >50% confluency was checked for ELISA titer value against the truncated HRP2 antigen.…”

Section: Methodsmentioning

confidence: 99%

Adjuvant poly(N‐isopropylacrylamide) generates more efficient monoclonal antibodies against truncated recombinant histidine‐rich protein2 of Plasmodium falciparum for malaria diagnosis

Verma

Ravichandran

Jayaprakash

et al. 2015

Biotechnology Journal

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Adjuvants play an important role in eliciting immune responses and subsequent generation of antibodies with high specificity. Recently, poly(N-isopropylacrylamide) (PNiPAAm), also known as a "smart" polymer, has been proposed as a potential adjuvant for making antibodies and vaccines. This material exhibits efficient delivery, protection against degradation, and preservation of antigen epitopes. In this work, we used both CFA and smart polymer to develop a highly specific murine monoclonal antibody (mAb) against recombinant truncated histidine rich protein2 (HRP2) of Plasmodium falciparum. Our results indicate that the mAbs developed using these adjuvants were able to recognize recombinant HRP2 and native PfHRP2 protein from spent medium. The mAbs generated against recombinant truncated HRP2 showed better sensitivity to the antigen and importantly mAbs generated using PNiPAAm adjuvant were in the range of 10(8)-10(9) M(-1). The mAbs generated using PNiPAAm are very efficient and sensitive in detecting HRP2. To the best of our knowledge, this is the first report of such comparison having been made between these two adjuvants and we propose that the smart polymer has huge potential as an alternative to CFA. Additionally, we discuss the utility of the mAbs generated through PNiPAAm for specific diagnosis of malaria caused by P. falciparum.

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Section: Methodsmentioning

confidence: 99%

Adjuvant poly(N‐isopropylacrylamide) generates more efficient monoclonal antibodies against truncated recombinant histidine‐rich protein2 of Plasmodium falciparum for malaria diagnosis

Verma

Ravichandran

Jayaprakash

et al. 2015

Biotechnology Journal

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“…40 To overcome this challenge and achieve monoclonality and consistent antibody secretion, single-cell cloning was performed. 40,41 This process led to the identication of four cell lines, namely 1C1, 2C2, 3A9, and 4A11, which demonstrated the ability to consistently secrete antibodies against EDTA-As 3+ . Following freezing and revival, the supernatants from these cell lines were diluted at different ratios (0, 3, 5, and 15 times), and the resulting OD values were measured.…”

Section: Purication and Identication Of Mabs Against As 3+mentioning

confidence: 99%

Preparation of arsenic(iii) monoclonal antibodies and preliminary evaluation of a novel silver-coated gold nanorod SERS immunoassay strip construction

Liang,

Li,

et al. 2023

Anal. Methods

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“…Human serum albumin (HSA) is a single‐chain nonglycosylated protein, accounting for more than 50% of the content of serum protein in the human body. [ 1–3 ] It plays an important role in maintaining osmotic pressure, buffering blood pH value, and transporting hormones, amino acids, fatty acids, and others to target organs. [ 4,5 ] It is the main product of hepatocytes and can be used for the treatment of some diseases such as hypovolemia and hypoproteinaemia.…”

Section: Introductionmentioning

confidence: 99%

A novel 1,8‐naphthalimide‐based fluorescent chemosensor for the detection of HSA in living cells

Tang

et al. 2022

Luminescence

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Human serum albumin (HSA) is an essential protein for maintaining human health. Accurate detection and quantification of HSA are of great significance for disease diagnosis and biochemical research. Here, a new HSA fluorescent probe BNPE based on the 1,8-naphthalimide fluorophore was designed and synthesized. The probe could recognize HSA through a twisted intramolecular charge transfer mechanism, effectively avoid the interference of most substances, and realize HSA fluorescence imaging in living cells.

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Production and characterization of monoclonal antibodies (mAbs) against human serum albumin (HSA) for the development of an immunoaffinity system with oriented anti-HSA mAbs as immobilized ligand

Cited by 7 publications

References 34 publications

Adjuvant poly(N‐isopropylacrylamide) generates more efficient monoclonal antibodies against truncated recombinant histidine‐rich protein2 of Plasmodium falciparum for malaria diagnosis

Adjuvant poly(N‐isopropylacrylamide) generates more efficient monoclonal antibodies against truncated recombinant histidine‐rich protein2 of Plasmodium falciparum for malaria diagnosis

Preparation of arsenic(iii) monoclonal antibodies and preliminary evaluation of a novel silver-coated gold nanorod SERS immunoassay strip construction

A novel 1,8‐naphthalimide‐based fluorescent chemosensor for the detection of HSA in living cells

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