Production and characterization of monoclonal antibodies to Aeromanas sobria surface antigens

Nandapalan, N.

doi:10.1016/0378-1097(89)90281-4

Cited by 8 publications

(14 citation statements)

References 20 publications

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“…2). Western blotting with mAb 23A9 which is specific for the O side chain of LPS [12] confirmed that 3767T is a rough isolate lacking the O side chain (Fig. 3, lane 4) while 37670 is smooth (Fig.…”

Section: Expression Of Lps In 37670 and 3767tmentioning

confidence: 78%

“…After electrophoresis, gels were processed for Western blotting onto nitrocellulose (NC) membrane according to Towbin et al [15]. NC membranes were blocked by incubation with 1% skim milk for 30 min at 37 °, and immunostained with mAb 23A9 [12] and alkaline phosphatase conjugated goat anti-mouse immunoglobulin. The substrate solution consisted of/3-naphthyl phosphate and tetrazotized O-dianisidine (Sigma).…”

Section: Sds-page and Western Blottingmentioning

confidence: 99%

“…sobria' 3767), a strain isolated from the faeces of a patient with diarrhoea [11]. Both protein and LPS have been implicated as adhesins in this strain [12]. Two colony variants were noticed on blood agar plates following initial isolation of A. sobria 3767; opaque colonies (37670) and translucent colonies (3767T).…”

Section: Introductionmentioning

confidence: 99%

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Variable expression of O-antigen and the role of lipopolysaccharide as an adhesin inAeromonas sobria

Francki

Chang

1994

FEMS Microbiology Letters

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On initial isolation of Aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767O) and translucent (3767T). Strain 3767O consistently produced lipopolysaccharide (LPS) core and O-antigen side chain, detectable by SDS-PAGE and by Western blotting with an O-antigen-specific monoclonal antibody. Strain 3767T produced LPS core but the amount of O-antigen was dependent on factors including growth medium and bacterial growth phase. Strain 3767T exhibited significantly lower levels of adhesion to HEp-2 cells than 3767O and this correlated with the level of LPS expression, with the greatest reduction (61%) at stationary phase when no LPS was detectable. The results implicate LPS as an adhesin for A. sobria 3767.

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Section: Expression Of Lps In 37670 and 3767tmentioning

confidence: 78%

Section: Sds-page and Western Blottingmentioning

confidence: 99%

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Variable expression of O-antigen and the role of lipopolysaccharide as an adhesin inAeromonas sobria

Francki

Chang

1994

FEMS Microbiology Letters

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“…Studies of antigen expression of A. hydrophila in biofilms have revealed that a complete LPS structure is important for the ability of Aeromonas species to maintain the proper structure of the tetragonal S -layer on the cell surface, and the structural changes in the LPS molecule can result in the loss of appropriate conformation of the protein structure [15]. Moreover, the O -specific polysaccharide (OPS) was implicated as a factor promoting adhesion of Aeromonas bacilli to HEp-2 cells, and switching off of the OPS by using specific monoclonal antibodies significantly reduced bacterial adherence [16,17]. …”

Section: Introductionmentioning

confidence: 99%

Structural and Immunochemical Studies of the Lipopolysaccharide from the Fish Pathogen, Aeromonas bestiarum Strain K296, Serotype O18

et al. 2013

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Chemical analyses and mass spectrometry were used to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas bestiarum strain K296, serotype O18. ESI-MS revealed that the most abundant A. bestiarum LPS glycoforms have a hexa-acylated or tetra-acylated lipid A with conserved architecture of the backbone, consisting of a 1,4′-bisphosphorylated β-(1→6)-linked d-GlcN disaccharide with an AraN residue as a non-stoichiometric substituent and a core oligosaccharide composed of Kdo1Hep6Hex1HexN1P1. 1D and 2D NMR spectroscopy revealed that the O-specific polysaccharide (OPS) of A. bestiarum K296 consists of a branched tetrasaccharide repeating unit containing two 6-deoxy-l-talose (6dTalp), one Manp and one GalpNAc residues; thus, it is similar to that of the OPS of A. hydrophila AH-3 (serotype O34) in both the sugar composition and the glycosylation pattern. Moreover, 3-substituted 6dTalp was 2-O-acetylated and additional O-acetyl groups were identified at O-2 and O-4 (or O-3) positions of the terminal 6dTalp. Western blots with polyclonal rabbit sera showed that serotypes O18 and O34 share some epitopes in the LPS. The very weak reaction of the anti-O34 serum with the O-deacylated LPS of A. bestiarum K296 might have been due to the different O-acetylation pattern of the terminal 6dTalp. The latter suggestion was further confirmed by NMR.

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“…Little is known about Aeromonas intestinal colonization factors. Both filamentous (pill) and outer membrane adhesins have been described [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Pili and the interaction of Aeromonas species with humanperipheral blood polymorphonuclear cells

Kamperman¹

1993

FEMS Immunology and Medical Microbiology

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The interaction of differentially piliated Aeromonas strains expressing pili of two broadly different morphologic types (short, rigid (S/R) and/or long, wavy (L/W)) with human peripheral blood mononuclear leukocytes (PMN) was investigated to determine whether host defense cells might exert a selective pressure on pili expression in vivo accounting for the different pili phenotypes of clinical and environmental strains. A majority of Aeromonas veronii biotype sobria strains from water (6/6) and faeces (8/11) readily associated with PMN (> 60% PMN with adherent and/or internalised bacteria), irrespective of their degree, or predominant type, of piliation. Rigid pili of Aeromonas species did not promote interaction with PMN. However, the majority (55%) of strains which interacted well with PMN were adherent to HEp-2 cells. Interaction with PMN is unlikely to be the reason few S/R pili are seen on faecal strains, but it may be a selective pressure on L/W adhesive pili, or other OMP adhesins, resulting in the shedding of strains which have lost critical adhesins.

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Production and characterization of monoclonal antibodies to Aeromanas sobria surface antigens

Cited by 8 publications

References 20 publications

Variable expression of O-antigen and the role of lipopolysaccharide as an adhesin inAeromonas sobria

Variable expression of O-antigen and the role of lipopolysaccharide as an adhesin inAeromonas sobria

Structural and Immunochemical Studies of the Lipopolysaccharide from the Fish Pathogen, Aeromonas bestiarum Strain K296, Serotype O18

Pili and the interaction of Aeromonas species with humanperipheral blood polymorphonuclear cells

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