Production and characterization of liquid-stored and lyophilized reconstituted human infusible platelet membranes

Abstract

This study revealed the potential of IPM microvesicles to mimic the binding features of platelets in vitro.

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

“…Whereas we found that the maximum binding of IPM to vWF occurred with the IPM prepared in the 0.25 or 0.4 M concentration of sodium octanoate. So, the ability of IPM to bind to vWF was dependent on the concentration of the stabilizer [18].…”

“…Platelets were used to produce IPM as it was reported before [18]; briefly; these cells were lysed by replicate freezing and thawing for 3 times, virally inactivated by moist heat at 60°C for 20 h with different concentrations of sodium octanoate (0, 0.1, 0.25, 0.4, 0.6 M). IPM were divided into vials and kept in refrigerator.…”

Glycoproteins of GpIbα and GpIIbIIIa on the Synthetic or Naturally Occurred Platelet-Derived Microparticles

“…The expression of gpIIb/IIIa in this study could not be attributed to the platelets remained in the IPM preparation because the size of prepared IPM microparticles was determined to be in the range of 84.7-291 nm (Z-average = 174 nm) [15] that had no correlation with the size of platelets.…”

“…Alternatively, the main cause of the difference may be related to the preparation method as its effect was elucidated in our study using different concentrations of the heat stabilizer but the use of heat protection method were not explained in the work of Chao. Our previous study showed that during the heating step of IPM preparation the usage of a heat stabilizer (sodium octanoate) was necessary to obtain the maximum vWF binding activity of IPM [15].…”

“…Each time five units of platelet concentrates were used for the production of IPM. Preparation of the IPM microparticles was accomplished as mentioned before [15]. Briefly, the cells were lysed by three times by freezing and thawing.…”