Production and Characterization of Iturinic Lipopeptides as Antifungal Agents and Biosurfactants Produced by a Marine Pinctada martensii-Derived Bacillus mojavensis B0621A

Abstract

Bacillus mojavensis B0621A was isolated from a pearl oyster Pinctada martensii collected from South China Sea. While screening for cyclic lipopeptides potentially useful as lead compounds for biological control against soil-bone fungal plant pathogens, three lipopeptides were isolated and purified from the fermentation broth of B. mojavensis B0621A via vacuum flash chromatography coupled with reversed-phase high performance liquid chromatography (RP-HPLC). The structural characterization and identification of these cyclic lipopeptides were performed by tandem mass spectrometry (MS/MS) combined with gas chromatography-mass spectrometry (GC-MS) analysis as well as chemical degradation. These lipopeptides were finally characterized as homologues of mojavensins, which contained identical amino acids back bones of asparagine1, tyrosine2, asparagine3, glutamine4, proline5, asparagine6, and asparagine7 and differed from each other by their saturated β-amino fatty acid chain residues, namely, iso-C14 mojavensin, iso-C16 mojavensin, and anteiso-C17 mojavensin, respectively. All lipopeptide isomers, especially iso-C16 mojavensin and anteiso-C17 mojavensin, displayed moderate antagonism and dose-dependent activity against several formae speciales of Fusarium oxysporum and presented surface tension activities. These properties demonstrated that the lipopeptides produced by B. mojavensis B0621A may be useful as biological control agent to fungal plant pathogens.

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

“…However, few studies reported the influence of culture media on lipopeptides production by Bacillus mojavensis strains. In fact, maximum production of lipopeptides by B. mojavensis B0621A was performed at 28°C for 48 h under shaking at 180 rpm in sucrose mineral salt medium [ 18 ].…”

Marine derived biosurfactants: a vast potential future resource

“…Due to their hydrophobic nature the longer fatty acid chain interact effectively with the cell membrane. As reported, among three different lipopeptides isolated from marine derived B. mojavensis , the antifungal activity of fengycins (C 16 and C 17 ) were stronger than mojavensin A (C 15 ) (Ma and Hu 2014 ; Ma et al 2012 ). Likewise, the longer fatty acid chain isoform C 16 of iturin A from marine Bacillus megaterium inhibited the proliferation of tumour cells by disrupting the Akt pathway leading to apoptosis (Dey et al 2015 ).…”

Saponins and microbial biosurfactants: Potential raw materials for the formulation of cosmetics

“…The selection of a biosurfactant for a specific cosmetic product is a delicate task that depends of various factors based on the intended purpose of the product . Some variables related to the properties of the biosurfactant should be taken into account.…”

“…11 The selection of a biosurfactant for a specific cosmetic product is a delicate task that depends of various factors based on the intended purpose of the product. 47 Some variables related to the properties of the biosurfactant should be taken into account. The CMC, hydrophilic-lipophilic balance (HLB), and ionic performance are the main properties that determine the type or use of biosurfactants in cosmetic formulations.…”