Production and characterization of interspecific somatic hybrids between Primula malacoides and P. obconica

Mizuhiro, Megumi; Ito, Kyoko; Mii, Masahiro

doi:10.1016/s0168-9452(01)00434-4

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…Mizuhiro et al (2001a) succeeded in plant regeneration from cell-suspension-derived protoplasts of P. malacoides and P. obconica. He produced somatic hybrids between these two species (Mizuhiro et al, 2001b). Plant regeneration from leaf calli of P. vulgaris and P. elatior was stimulated by a high level of TDZ (Schween and Schwenkel, 2003).…”

Section: Research Reportmentioning

confidence: 99%

In vitro seed germination and micropropagation of primrose (Primula heterochroma Stapf.) an endemic endangered Iranian species via shoot tip explants

Sharaf

Hamidoghli

Zakizadeh

2011

Hortic. Environ. Biotechnol.

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Primula heterochroma is a scarce and endemic plant to the north of Iran. The aim of the present study is to establish an efficient and reproducible protocol for in vitro propagation from shoot tips. Native seeds were exposed to different treatments. Germination results showed that the scarification of seeds had significant effects on the germination delay and germination percentage compared to plant growth regulator treatments. Afterwards, the seedling-derived shoot tips were cultured on several Murashige and Skoog basal medium, containing various concentrations of 6-benzylaminopurine (BA) or thidiazuron (TDZ) in combination with various concentrations of 1-naphthaleneacetic acid (NAA). The result showed that the highest shoot regeneration, and the number and length of leaves were noticeably affected by media enriched with 1 mg L -l BA + 0.4 mg L -l NAA and 0.2 mg L -l TDZ + 0.4 mg L -1 NAA. Root parameters significantly affected by media enriched with 1 mg L -1 BA + 0.8 mg L -1 NAA and 0.4 mg L -1 TDZ + 0.8 mg L -1 NAA. Plantlets were hardened-off and transferred to soil for further growth.Additional key words: in vitro propagation, plant growth regulators, primula spp., rare plant, shoot regeneration Hort. Environ. Biotechnol. 52 (3):298-302. 2011.

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Section: Research Reportmentioning

confidence: 99%

In vitro seed germination and micropropagation of primrose (Primula heterochroma Stapf.) an endemic endangered Iranian species via shoot tip explants

Sharaf

Hamidoghli

Zakizadeh

2011

Hortic. Environ. Biotechnol.

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“…Yamamoto et al (1999) obtained regenerated plants from young expanding leaves of P. sieboldii on media with BA and NAA. Mizuhiro et al (2001a) succeeded in plant regeneration from cell-suspension-derived protoplasts of P. malacoides and P. obconica, and produced somatic hybrids between them (Mizuhiro et al 2001b). Plant regeneration from leaf calli of P. vulgaris and P. elatior was stimulated by a high level of TDZ Schwenkel 2002, Schween andSchwenkel 2003).…”

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confidence: 99%

Plant regeneration from leaf explants of auricula cultivars (Primula*pubescens Jacq.)

Takihira

Otani

Tsuchiya

et al. 2007

Plant Biotechnology

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Cultivars of auricula (Primulaϫpubescens Jacq.) are descendants of a natural cross between P. auricula and P. hirsuta, which is a large family of plants comprising many thousands of hybrids. They have become popular as pot flowers in not only England but also other countries. All cultivars of auricula are hybrids and vegetative propagated by taking offsets from donor plants. Establishment of system for plant regeneration from cultured cells and tissues is necessary for micropropagating new cultivars and conserving the valuable old cultivars in danger of extinction.There have been a few reports on plant regeneration from cultured cells and tissues of Primula species. Coumans et al. (1979) reported that young floral buds of P. obconica regenerated adventitious shoots when cultured on media with BA and NAA. Shimada et al. (1997) reported that regeneration of adventitious shoots and somatic embryos from leaf explants of P. cuneifolia var. hakusanensis was stimulated by TDZ or zeatin. Yamamoto et al. (1999) obtained regenerated plants from young expanding leaves of P. sieboldii on media with BA and NAA. Mizuhiro et al. (2001a) succeeded in plant regeneration from cell-suspension-derived protoplasts of P. malacoides and P. obconica, and produced somatic hybrids between them (Mizuhiro et al. 2001b). Plant regeneration from leaf calli of P. vulgaris and P. elatior was stimulated by a high level of TDZ Schwenkel 2002, Schween andSchwenkel 2003). In the present study, we investigated the effect of cytokinins on adventitious shoot regeneration from leaf explants of auricula cultivars.Four auricula cultivars, 'Borders Mixed', 'Border Stripes', 'Alpines Mixed' and 'Field House Mixed', were used in the present study. Seeds of each cultivar were kept in a refrigerator (4°C) until use. Seeds were surfacesterilized with a NaOCl solution containing 3% (v/v) active chlorine for 15 min followed by rinsing three times with sterilized distilled water, and then sown on plant growth regulator-free LS (Linsmaier and Skoog 1965) medium supplemented with 30 g l Ϫ1 sucrose and 2.5 g l Ϫ1 of gellan gum. After three-months of culture, seedlings grew into plantlets with 4 to 5 expanded leaves. Leaves were harvested from these plantlets, cut into 15ϫ15 mm pieces and placed on LS media supplemented with 30 g l Ϫ1 sucrose, 2.5 g l Ϫ1 gellan gum and various concentrations (0-2 mg l Ϫ1) and combinations of cytokinins (BA, Zeatin or TDZ) and NAA. For each medium, 30 leaf explants were cultured. Plant growth regulators were added to the medium and the medium was adjusted to pH 5.9 prior to autoclaving. All cultures were incubated at 20°C under a 14-h photoperiod with fluorescent lighting (about 3,000 lux). Abstract Adventitious shoots were regenerated from leaf explants of 4 cultivars of auricula (Primulaϫpubescens Jacq.) on media supplemented with various cytokinins combined with NAA. One to four adventitious shoots per leaf explant were regenerated. The highest frequency of adventitious shoot formation was 46.6% for 'Borders Mixed' on the medium c...

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“…Increase in the DNA content was observed in somatic hybrids of Solanum brevidens (+) S. tuberosum (Puite and Schaart 1993), Primula malacoides (+) P. obconica (Mizuhiro et al 2001), and Diospyros glandulosa (+) D. kaki (Tamura et al 1998). It was suggested that chromosome doubling occurred during callus proliferation.…”

Section: Nuclear Dna Content and Chromosome Number And Its Influence mentioning

confidence: 99%

“…Some hybrid lines of P. malacoides (+) P. obconica showed a decrease in DNA content during the plant regeneration process (Mizuhiro et al 2001). More recently, Guo et al (2010) reported that somatic hybrids between S. tuberosum and S. chacoense underwent gradual DNA elimination during 3 years of in vitro culture.…”

Section: Nuclear Dna Content and Chromosome Number And Its Influence mentioning

confidence: 99%

“…However, regeneration of plants from fused protoplasts is often accompanied by polyploidization and/or the elimination of genome parts of either one or both fusion parents (Babiychuk et al 1992;Oberwalder et al 1998;Mizuhiro et al 2001;Guo et al 2010). Thus, detailed investigation is needed, not only to confirm the hybrid status of regenerants, but also to characterize precisely their genome size and composition, which is essential for their further efficient exploitation (SmydaDajmund et al 2016).…”

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confidence: 99%

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Protoplast fusion in the genus Gentiana: genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata L.

Tomiczak

Śliwińska

Rybczyński

2017

Plant Cell Tiss Organ Cult

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Production and characterization of interspecific somatic hybrids between Primula malacoides and P. obconica

Cited by 15 publications

References 19 publications

In vitro seed germination and micropropagation of primrose (Primula heterochroma Stapf.) an endemic endangered Iranian species via shoot tip explants

In vitro seed germination and micropropagation of primrose (Primula heterochroma Stapf.) an endemic endangered Iranian species via shoot tip explants

Plant regeneration from leaf explants of auricula cultivars (Primula*pubescens Jacq.)

Protoplast fusion in the genus Gentiana: genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata L.

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