Production and characterization of elastomeric cardiac tissue-like patches for Myocardial Tissue Engineering

Cesur, Sümeyye; Ulag, Songul; Ozak, Lara; Gumussoy, Aleyna; Arslan, Sema; Yılmaz, Baran; Ekren, Nazmi; Ağırbaşlı, Mehmet; Kalaskar, Deepak M.; Gündüz, Oğuzhan

doi:10.1016/j.polymertesting.2020.106613

Cited by 41 publications

(24 citation statements)

References 39 publications

(27 reference statements)

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“…Figure 3 B,a shows the FTIR spectrum of the PLA microfiber. The main absorption peaks have been reported to be at ~1770 cm −1 (C=O vibration), ~1450 cm −1 (CH 3 asymmetrical scissoring), ~1080 cm −1 (C-O-C stretching), ~1040 cm −1 (C–CH 3 stretching), and ~860 cm −1 (C–COO stretching) [ 40 ]. Some shifts were detected with 0.1, 0.5, and 1 wt.% GO addition, proving that GO addition has an important impact because of the strong interactions developing between GO and PLA ( Figure 3 B,b,c,d).…”

Section: Resultsmentioning

confidence: 99%

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Electrically Triggered Drug Delivery from Novel Electrospun Poly(Lactic Acid)/Graphene Oxide/Quercetin Fibrous Scaffolds for Wound Dressing Applications

et al. 2021

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The novel controlled and localized delivery of drug molecules to target tissues using an external electric stimulus makes electro-responsive drug delivery systems both feasible and desirable, as well as entailing a reduction in the side effects. Novel micro-scaffold matrices were designed based on poly(lactic acid) (PLA) and graphene oxide (GO) via electrospinning. Quercetin (Q), a natural flavonoid, was loaded into the fiber matrices in order to investigate the potential as a model drug for wound dressing applications. The physico-chemical properties, electrical triggering capacity, antimicrobial assay and biocompatibility were also investigated. The newly fabricated PLA/GO/Q scaffolds showed uniform and smooth surface morphologies, without any beads, and with diameters ranging from 1107 nm (10%PLA/0.1GO/Q) to 1243 nm (10%PLA). The in vitro release tests of Q from the scaffolds showed that Q can be released much faster (up to 8640 times) when an appropriate electric field is applied compared to traditional drug-release approaches. For instance, 10 s of electric stimulation is enough to ensure the full delivery of the loaded Q from the 10%PLA/1%GO/Q microfiber scaffold at both 10 Hz and at 50 Hz. The antimicrobial tests showed the inhibition of bacterial film growth. Certainly, these materials could be loaded with more potent agents for anti-cancer, anti-infection, and anti-osteoporotic therapies. The L929 fibroblast cells cultured on these scaffolds were distributed homogeneously on the scaffolds, and the highest viability value of 82.3% was obtained for the 10%PLA/0.5%GO/Q microfiber scaffold. Moreover, the addition of Q in the PLA/GO matrix stimulated the production of IL-6 at 24 h, which could be linked to an acute inflammatory response in the exposed fibroblast cells, as a potential effect of wound healing. As a general conclusion, these results demonstrate the possibility of developing graphene oxide-based supports for the electrically triggered delivery of biological active agents, with the delivery rate being externally controlled in order to ensure personalized release.

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Section: Resultsmentioning

confidence: 99%

“…(C-COO stretching) [40]. Some shifts were detected with 0.1, 0.5, and 1 wt.% GO addition, proving that GO addition has an important impact because of the strong interactions developing between GO and PLA (Figure 3B,b,c,d).…”

Section: Ftir Microscopymentioning

confidence: 86%

Electrically Triggered Drug Delivery from Novel Electrospun Poly(Lactic Acid)/Graphene Oxide/Quercetin Fibrous Scaffolds for Wound Dressing Applications

et al. 2021

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“…The molecular structures of pure PLA, CS, and levodopa and all scaffold samples produced by 3D-printing are given in Figure 2. The main characteristic absorption bands are C=O vibration peak at 1750 cm −1 , CH3 asymmetric shear at 1456 cm −1 , CH3 and C-H bending vibrations 1386 and 1358 cm −1 , C-O-C stretching vibration at 1265 cm −1 , C-O-C stretching at 1080 cm −1 , C-CH3 stretching, OH bending at 950 cm −1 and C-COO stretching at 873 cm −1 for pure PLA in Figure 2a, respectively [26]. Figure 2b shows a large peak at approximately 3283 cm −1 , representing hydrogen-bonded O-H or N-H stretch in pure CS, while the peak close to 2869 cm −1 reflects the C-H vibration.…”

Section: Fourier Transform-infrared Spectroscopy (Ft-ir)mentioning

confidence: 97%

Levodopa-Loaded 3D-Printed Poly (Lactic) Acid/Chitosan Neural Tissue Scaffold as a Promising Drug Delivery System for the Treatment of Parkinson’s Disease

et al. 2021

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Parkinson’s disease, the second most common neurodegenerative disease in the world, develops due to decreased dopamine levels in the basal ganglia. Levodopa, a dopamine precursor used in the treatment of Parkinson’s disease, can be used as a drug delivery system. This study presents an approach to the use of 3D-printed levodopa-loaded neural tissue scaffolds produced with polylactic acid (PLA) and chitosan (CS) for the treatment of Parkinson’s disease. Surface morphology and pore sizes were examined by scanning electron microscopy (SEM). Average pore sizes of 100–200 µm were found to be ideal for tissue engineering scaffolds, allowing cell penetration but not drastically altering the mechanical properties. It was observed that the swelling and weight loss behaviors of the scaffolds increased after the addition of CS to the PLA. Levodopa was released from the 3D-printed scaffolds in a controlled manner for 14 days, according to a Fickian diffusion mechanism. Mesenchymal stem cells (hAD-MSCs) derived from human adipose tissue were used in MTT analysis, fluorescence microscopy and SEM studies and confirmed adequate biocompatibility. Overall, the obtained results show that PLA/CS 3D-printed scaffolds have an alternative use for the levodopa delivery system for Parkinson’s disease in neural tissue engineering applications.

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“…Cardiac-tissue-like patches are also an interesting approach, as reported by Cesur et al [ 89 ], in which a randomly oriented PLA, PLA/PEG, and random and aligned PLA/PEG/collagen (PLA/PEG/COL) nanofiber patches were successfully produced by the electrospinning technique for myocardial tissue engineering, which is one of the promising treatment modalities for repairing damaged heart tissue in patients with heart failure. Interestingly, as reported, even a small amount of COL (1 wt %) in a PEG-plasticized PLA could increase the electrical conductivity by two orders of magnitude.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Special Features of Polyester-Based Materials for Medical Applications

Darie-Niță¹,

Râpă

Frąckowiak

2022

Polymers

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This article presents current possibilities of using polyester-based materials in hard and soft tissue engineering, wound dressings, surgical implants, vascular reconstructive surgery, ophthalmology, and other medical applications. The review summarizes the recent literature on the key features of processing methods and potential suitable combinations of polyester-based materials with improved physicochemical and biological properties that meet the specific requirements for selected medical fields. The polyester materials used in multiresistant infection prevention, including during the COVID-19 pandemic, as well as aspects covering environmental concerns, current risks and limitations, and potential future directions are also addressed. Depending on the different features of polyester types, as well as their specific medical applications, it can be generally estimated that 25–50% polyesters are used in the medical field, while an increase of at least 20% has been achieved since the COVID-19 pandemic started. The remaining percentage is provided by other types of natural or synthetic polymers; i.e., 25% polyolefins in personal protection equipment (PPE).

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Production and characterization of elastomeric cardiac tissue-like patches for Myocardial Tissue Engineering

Cited by 41 publications

References 39 publications

Electrically Triggered Drug Delivery from Novel Electrospun Poly(Lactic Acid)/Graphene Oxide/Quercetin Fibrous Scaffolds for Wound Dressing Applications

Electrically Triggered Drug Delivery from Novel Electrospun Poly(Lactic Acid)/Graphene Oxide/Quercetin Fibrous Scaffolds for Wound Dressing Applications

Levodopa-Loaded 3D-Printed Poly (Lactic) Acid/Chitosan Neural Tissue Scaffold as a Promising Drug Delivery System for the Treatment of Parkinson’s Disease

Special Features of Polyester-Based Materials for Medical Applications

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