Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration

Serra, I R; Fradique, Ricardo; Vallejo, Mariana Costa da Silva; Correia, Tiago Ruivo; Miguel, Sónia P.; Correia, Ilídio J.

doi:10.1016/j.msec.2015.05.072

Cited by 138 publications

(83 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 1A shows the images of PBSu and om-CMS/PBSu scaffolds. The density of the PBSu scaffold increases from 1.26 to 1.42 g/cm 3 after blending with the om-CMS powder. Figure 1B and C represents transmission electron microscopy images revealing the morphology and microstructure of the om-CMS/PBSu scaffold.…”

Section: Resultsmentioning

confidence: 99%

“…16 Briefly, 4 g of Pluronic P123 (PEO 20 3 were added to the solution. This was followed by addition of 8.5 g of tetraethyl orthosilicate dropwise using an injection pump.…”

Section: Methodsmentioning

confidence: 99%

“…3 Scaffolds have certain advantages over autologous grafts, such as easy shaping, limitless supply, and avoidance of donor-site morbidity. Biodegradable scaffolds even allow new tissues to take over the biological functions; therefore, potential chronic problems associated with biostable implants can be avoided.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradable mesoporous calcium–magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Zhang¹,

Zhang²,

Xu³

et al. 2015

IJN

View full text Add to dashboard Cite

The structural features of bone engineering scaffolds are expected to exhibit osteoinductive behavior and promote cell adhesion, proliferation, and differentiation. In the present study, we employed synthesized ordered mesoporous calcium–magnesium silicate (om-CMS) and polybutylene succinate (PBSu) to develop a novel scaffold with potential applications in osseous tissue engineering. The characteristics, in vitro bioactivity of om-CMS/PBSu scaffold, as well as the cellular responses of MC3T3-E1 cells to the composite were investigated. Our results showed that the om-CMS/PBSu scaffold possesses a large surface area and highly ordered channel pores, resulting in improved degradation and biocompatibility compared to the PBSu scaffold. Moreover, the om-CMS/PBSu scaffold exhibited significantly higher bioactivity and induced apatite formation on its surface after immersion in the simulated body fluid. In addition, the om-CMS/PBSu scaffold provided a high surface area for cell attachment and released Ca, Mg, and Si ions to stimulate osteoblast proliferation. The unique surface characteristics and higher biological efficacy of the om-CMS/PBSu scaffold suggest that it has great potential for being developed into a system that can be employed in osseous tissue engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

“…16 Briefly, 4 g of Pluronic P123 (PEO 20 3 were added to the solution. This was followed by addition of 8.5 g of tetraethyl orthosilicate dropwise using an injection pump.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradable mesoporous calcium–magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Zhang¹,

Zhang²,

Xu³

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…A porous gelatin-chitosan-β-TCP scaffold prepared by khan et al demonstrated improved mechanical property and in vitro biocompatibility [25]. Moreover, gelatin-chitosan-β-TCP based scaffolds prepared by Serra et al exhibited in vitro differentiation of Human Osteoblast Cell (HOB) and good antibacterial activity which prevented them from risk of inflammatory response [26]. However, no study has yet been performed to study the effect of b-TCP nanoparticles on microstructure, protein adsorption capacity and biodegradability of gelatin-chitosan-b TCP based scaffold.…”

Section: Introductionmentioning

confidence: 99%

Effect of βTricalcium Phosphate Nanoparticles Additions on the Properties of Gelatin-Chitosan Scaffolds

Maji¹,

Dasgupta²

2017

Bioceram Dev Appl

View full text Add to dashboard Cite

Bone tissue engineering, using a synthetic porous scaffold material provides some distinct advantages over autografting and allografting, and it is a rapidly growing alternative approach to heal damaged bone tissue. The current study focuses on fabrication and characterization of nano β-TCP incorporated gelatin-chitosan based composite scaffold for bone regeneration at the sites of musculoskeletal defects and disorders.Gelatin-chitosan scaffold reinforced with beta-tricalcium phosphate (β-TCP) nanopowder was fabricated through freeze drying of material's suspension. From powder X-ray diffraction and Fourier transform infrared spectrometer analysis the presence of phase pure β-TCP powders in gelatin-chitosan matrix was confirmed. Gelatin-Chitosan-β-TCP (GCT) scaffold exhibited a homogenouos porous structure with an average pore size of 118 ± 11 µm. Micro-CT image confirmed interconnected porous network with homogeneous distribution of β-TCP nanoparticles in GelatinChitosan (GC) matrix. GCT scaffold showed higher compressive strength of 2.45 ± 0.15 MPa as compared to 1 MPa exhibited by neat GC scaffold. Protein adsorption capacity was increased to 22 mg/cc in GCT scaffold from 13 mg/ cc in GC scaffold. Weight loss of GCT scaffold was lower of 26% as compared to 47% in GC scaffold after 8 weeks of incubation in phosphate buffer solution of pH 7.4. Mesenchymal stem cells cultured onto GCT scaffold exhibited higher degree of lamellipodia and filopodia extensions and greater spreading onto GCT scaffold as compared to that in GC scaffold after 7 and 14 days of culture. MTT assay suggested higher degree of proliferation of MSCs cultured onto GCT scaffold as compared to that onto pure GC scaffolds. This study demonstrates that β-TCP incorporation into gelatin-chitosan matrix improved osteogenic potential of the scaffold suitable for bone tissue engineering.

show abstract

“…Therefore blending gelatin and chitosan may promote cellular functioning of scaffolds. There are several reports in the literature about these blends, describing a wide variety of engineering applications such as cartilage tissue engineering, supporting stem cells, hepatocyte culture, bone, and human meniscus tissue engineering, skin tissue regeneration and nerve regeneration . The main limitation of gelatin arises from its dissolution in aqueous environments, so other studies specify the use of crosslinking agents for these blends.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible scaffolds composed of chemically crosslinked chitosan and gelatin for tissue engineering

Cañas

Delgado

Gartner

2016

J of Applied Polymer Sci

View full text Add to dashboard Cite

Chitosan-based scaffolds are widely studied in tissue regeneration because of their biocompatibility and biodegradability. Scaffolds are obtained by different techniques and can be modified with other polymers allowing controlling their properties. This article discusses the assembling of three-dimensional chitosan porous scaffolds blended with gelatin. Gelatin was used to enhance cells attachment due to the presence of cell adhesion motifs, while improving mechanical strength. 2,5-dimethoxy-2,5-dihydrofurane (DHF) was used as the crosslinking agent, because it allowed to control the reaction kinetics through temperature, time and DHF concentration. The results indicate that scaffolds morphology, pore sizes and distribution, compressive moduli and biodegradation in vitro with lysozyme, can be customized with variations of gelatin content and crosslinking degree. Scaffolds were neither cytotoxic nor genotoxic for human keratinocytes, exhibiting cell-substrate interactions. Our findings demonstrated that chitosan-gelatin scaffolds crosslinked with DHF, as a new crosslinking agent, are suitable in tissue engineering applications. V C 2016 Wiley Periodicals, Inc. J.Appl. Polym. Sci. 2016, 133, 43814.

show abstract

Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration

Cited by 138 publications

References 79 publications

Biodegradable mesoporous calcium–magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Biodegradable mesoporous calcium–magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Effect of βTricalcium Phosphate Nanoparticles Additions on the Properties of Gelatin-Chitosan Scaffolds

Biocompatible scaffolds composed of chemically crosslinked chitosan and gelatin for tissue engineering

Contact Info

Product

Resources

About

Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration

Cited by 138 publications

References 79 publications

Biodegradable mesoporous calcium&ndash;magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Biodegradable mesoporous calcium&ndash;magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Effect of βTricalcium Phosphate Nanoparticles Additions on the Properties of Gelatin-Chitosan Scaffolds

Biocompatible scaffolds composed of chemically crosslinked chitosan and gelatin for tissue engineering

Contact Info

Product

Resources

About

Biodegradable mesoporous calcium–magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering

Biodegradable mesoporous calcium–magnesium silicate-polybutylene succinate scaffolds for osseous tissue engineering