Production and characterization of antisera against human macrophage Migration Inhibitory Factor (MIF)

The role of lymphokines in delayed-type hypersensitivity reactions

“…MIF from mouse, human, or guinea pig lymphocyte cultures appears to be an acidic glycoprotein which is heterogeneous in terms of molecular weight and isolelectric point [25,69,100,109,110,171,172,174,204,2057. The heterogeneity may be a result of the culture conditions [69,245] and/or to the monomericpolymeric nature of the molecules [100, 204,205,207,245]. The main MIF activities from stimulated guinea pig lymphocytes have been separated into two distinct species.…”

“…Anti-lymphokine antibodies raised against guinea pig and human MIF have been used to characterize the molecule both chemically and functionally [22,66,68,69,113,142,205,208]. Yoshida and colleagues [113,254] prepared antibodies against activated lymphocyte supernatant which had been absorbed with an antibody to a non-activated supernatant.…”