Production and characterization of an amphiploid between common wheat and Psathyrostachys huashanica Keng ex Kuo

Kang, Houyang; Wang, Y.; Sun, Guoxiang; Zhang, H. Q.; Fan, Xing; Zhou, Yong‐Hong

doi:10.1111/j.1439-0523.2008.01542.x

Cited by 32 publications

(52 citation statements)

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“…Since then, many stripe rust resistance genes of its wild relatives have been introduced into wheat using wide crosses [Riley et al, 1968;Uauy et al, 2005;Lin et al, 2009]. Although stripe rust resistance in P. huashanica has been reported in translocation lines and amphiploids [Cao et al, 2005;Kang et al, 2009], the chromosomal location of the stripe rust resistant gene(s) in P. huashanica has not been reported so far. Further studies are needed to determine whether the resistance genes in our lines are the same or different from that reported previously.…”

Section: Discussionmentioning

confidence: 99%

“…During the past 50 years, a large number of hybridizations between wheat and its wild relatives has been made for desirable genes transfer [Sears, 1956;Sharma and Gill, 1983;Chen et al, 1991;Sun et al, 1992;Jiang et al, 1994;Sharma, 1995;Kang et al, 2008Kang et al, , 2009. Numerous wheat-wild species derivatives have been produced from these crosses, such as wheat-alien species amphiploids, wheat-alien chromosome addition, substitution, and translocation lines [Shepherd and Islam, 1988;Fedak, 1999;Friebe et al, 2000;Cai et al, 2005;Kang et al, 2009]. The addition of alien chromosomes to the wheat genome has been known to serve as a bridge for the transfer of useful genetic material and hence a desired trait [Knott et al, 1977;Dosba et al, 1978;Doussinault et al, 1983;Fedak, 1999;Khan, 2000;Jauhar et al, 2009].…”

Section: Discussionmentioning

confidence: 99%

“…tritici) , and powdery mildew (Blumeria graminis f. sp. tritici) [Chen et al, 1991;Jing et al, 1999;Wang and Shang, 2000;Cao et al, 2005;Kang et al, 2009], P. huashanica is an important species in the tribe Triticeae for wheat improvement and has been used to produce hybrids with bread wheat [Chen et al, 1991;Sun et al, 1992;Kang et al, 2008]. Nowadays, wheat alien addition lines and substitution lines involving the chromosomes 1Ns, 5Ns, and 6Ns have been developed [Wang and Shang, 2000;Fu et al, 2003;Zhao et al, 2004aZhao et al, , b, 2009, but none of wheat-P. huashanica alien lines were tested for resistance to stripe rust.…”

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The 3Ns Chromosome of Psathyrostachys huashanica Carries the Gene(s) Underlying Wheat Stripe Rust Resistance

Wang

Yu²,

Xie³

et al. 2011

Cytogenet Genome Res

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Stripe rust (Puccinia striiformis tritici (Pst)) is one of the most destructive diseases of wheat in the world. Exploiting and utilizing stripe rust resistance genes of wild species has become an essential strategy for resistance breeding. Psathyrostachyshuashanica Keng ex Kuo is a wild species in Triticeae that has been used for wheat improvement because of its high resistance or immunity to stripe rust. In this study, 9 wheat-P. huashanica addition lines were characterized by Giemsa C-banding, genomic in situ hybridization (GISH), and disease resistance evaluation. Giemsa C-banding and GISH demonstrated that lines 163-5, 165-1, 183-5, 240-3, and 240-4 are P. huashanica 3Ns chromosome monosomic addition lines; lines 183-1 and 183-20 are P. huashanica 3Ns chromosome disomic addition lines; line 165-20 is a P. huashanica 3Ns and 4Ns chromosomes double disomic addition line, and line 219-1 is a P. huashanica 1Ns and 3Ns/5A chromosomes double disomic addition-substitution line. All these addition lines with P. huashanica 3Ns chromosome(s) expressed high resistance or immunity to stripe rust. By comparing the series of wheat-P. huashanica chromosome addition lines, we concluded that the P. huashanica 3Ns chromosome carries the gene(s) for resistance or immunity to stripe rust. These addition lines can be used as a donor source of novel stripe rust resistance to wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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The 3Ns Chromosome of Psathyrostachys huashanica Carries the Gene(s) Underlying Wheat Stripe Rust Resistance

Wang

Yu²,

Xie³

et al. 2011

Cytogenet Genome Res

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“…It possesses many desirable traits, such as early maturity, more spikelets per spike, tolerance to drought and salt, and resistance to wheat stripe rust, leaf rust, powdery mildew and take-all fungus [Chen et al, 1991;Jing et al, 1999;Wang and Shang, 2000;Kang et al, 2009;Du et al, 2013]. Therefore, the P. huashanica Ns genome can be used as a donor to provide superior genes for the genetic improvement of wheat crops.…”

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confidence: 99%

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and Psathyrostachys huashanica

Kang

Huang²,

Zhu³

et al. 2016

Cytogenet Genome Res

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Trigeneric hybrids are commonly used as bridges to transfer genes from some wild species to cultivated wheat and to measure the genomic interaction between donor species. We previously reported that trigeneric germplasms were produced by crossing wheat-Psathyrostachys huashanica amphiploids (PHW-SA, 2n = 8x = 56, AABBDDNsNs) with hexaploid triticale (Zhongsi 828, 2n = 6x = 42, AABBRR). In the present study, chromosome pairing behavior and the genome constitution of the F₄ progenies of wheat-rye-P. huashanica trigeneric hybrids were studied. Cytological analysis showed that the chromosome number of F₄ progenies ranged from 39 to 46, and 57.5% of them had 42 chromosomes. The mean meiotic configuration of F₄ lines was 1.71 univalents, 20.26 bivalents, 0.04 trivalents, and 0.001 quadrivalents per pollen mother cell. Among the lines with 2n = 42, the average pairing configuration was 1.21 univalents, 16.22 ring bivalents, 4.16 rod bivalents, and 0.01 trivalents. This result indicated that these lines were cytologically stable. Other lines with 2n = 39, 40, 41, 43, 44, 45, and 46, bearing a high number of univalents or multivalents, showed abnormal meiotic behavior. Genomic in situ hybridization (GISH) revealed that all F₄ lines had 11-14 rye chromosomes, but no P. huashanica chromosomes. The complete set of 14 rye chromosomes was found in 19 lines. At meiosis, GISH detected 1-6 univalents with hybridization signals of rye in 13 lines. Bivalents with fluorescence signals were identified in each line, ranging from 3 to 7. A quadrivalent with hybridization signals was observed in only 1 line, K13-714-8. Lagging chromosomes, chromosome bridges, micronuclei, and chromosome fragments hybridizing with the probe were not discovered in any of the lines. These results inferred that the behavior of rye chromosomes was normal during meiosis. In addition, 21 lines of 2n = 42 (91.3%) with 12 or 14 rye chromosomes, always contained 6 or 7 bivalents bearing fluorescence signals. This suggested that the rye chromosomes exhibiting complete pairing in these lines were cytologically stable during meiosis and may therefore be considered as new hexaploid triticales. Thus, these lines might be potential materials for further hexaploid triticale improvement.

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A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines

Wang

Lü

et al. 2013

Span. j. agric. res.

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The genome of Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) contains many desirable characteristics, such as resistance to biotic and abiotic stresses, which make it suitable for wheat improvement (Kang et al., 2009). Several P. huashanica genes have already been introgressed successfully into the wheat (Triticum aestivum L.) genome (Zhao et al., 2010; Du et al., 2013a,b). In the 1990s, we generated (Chen et al., 1991(Chen et al., , 1996 the F 1 hybrid H881 (2n = 28, ABDNs) and a derived heptaploid H8911 (2n = 49, AABBDDNs) of common wheat cv. 7182 and P. huashanica (GenBank Acc. No. 0503383) via embryo culture, two backcrosses, and selfing. As a result, we generated a large and complex range of wheat-P. huashanica offspring that carried different chromosome number or structure variants, i.e., chromosome additions, deletions, and rearrangements. However, a major problem was the selection of recombinant plants derived from the large interbred population. After several years of screening and identification, a complete set of wheat-P. huashanica disomic addition lines (1Ns -7Ns, 2n = 44 = 22II) was developed. This was a repetitive and difficult work. Although cytogenetic techniques proved to be very useful tools for alien identification, they had limitations because of the small population number, the complexity of the experimental techniques, and envi- AbstractPsathyrostachys huashanica Keng is an endangered species that is endemic to China, which provides an important gene pool for wheat improvement. We developed a quick and reliable PCR-based diagnostic assay to accurately and efficiently detect P. huashanica DNA sequences from introgression lines, which was based on a species-specific marker derived from genomic DNA. The 900-bp PCR-amplified band used as a P. huashanica-specific RAPD marker was tested with 21 different plant species and was converted into a sequence-characterized amplified region (SCAR) marker by cloning and sequencing the selected fragments (pHs11). This SCAR marker, which was designated as RHS-23, could clearly distinguish the presence of P. huashanica DNA repetitive sequences in wheat-P. huashanica derivative lines. The specificity of the marker was validated using 21 different plant species and a complete set of wheat-P. huashanica disomic addition lines (1Ns -7Ns, 2n = 44 = 22II). This specific sequence targeted the Ns genome of P. huashanica and it was present in all the seven P. huashanica chromosomes. Therefore, this SCAR marker is specific for P. huashanica chromosomes and may be used in the identification of alien repetitive sequences in large gene pools. This diagnostic PCR assay for screening the target genetic material may play a key role in marker-assisted selective breeding programs.Additional key words: addition lines; marker-assisted selection; repetitive sequences; RAPD; SCAR.* Corresponding author: cxh2089@126.com; wj2105@163.com Received: 24-03-13. Accepted: 15-10-13.This work has one Supplementary Figure that does not appear in the printed article but that accompan...

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Production and characterization of an amphiploid between common wheat and Psathyrostachys huashanica Keng ex Kuo

Cited by 32 publications

References 15 publications

The 3Ns Chromosome of <i>Psathyrostachys huashanica</i> Carries the Gene(s) Underlying Wheat Stripe Rust Resistance

The 3Ns Chromosome of <i>Psathyrostachys huashanica</i> Carries the Gene(s) Underlying Wheat Stripe Rust Resistance

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and <b><i>Psathyrostachys huashanica</i></b>

A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines

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