2018
DOI: 10.1080/09540105.2018.1540550
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Production and characterization of a single-chain Fv antibody–alkaline phosphatase fusion protein specific for ampicillin

Abstract: Anti-AMP scFv were prepared by cloning variable region genes (VH and VL) from hybridoma cell line which secretes antibodies against AMP, and phoA gene was cloned from Escherichia coli strain K12 chromosomal DNA. The resulting scFv and phoA gene were inserted into the expression vector pBV220 to express the scFv-AP fusion protein in E. coli strain BL21. The purified scFv-AP fusion protein was used to develop an ic-CLEIA method for detection of AMP. The limit of detection (IC 15) was 0.11 ± 0.004 ng/mL and the I… Show more

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Cited by 10 publications
(4 citation statements)
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“…after the promoter induction. Finally, pellets were harvested by centrifugation of each sample at 7000 rpm, 10min at 22 °C, and were processed for further studies [ 18 , 19 ].…”
Section: Methodsmentioning
confidence: 99%
“…after the promoter induction. Finally, pellets were harvested by centrifugation of each sample at 7000 rpm, 10min at 22 °C, and were processed for further studies [ 18 , 19 ].…”
Section: Methodsmentioning
confidence: 99%
“…Wang et al, 2010), fluoroquinolones (Tao et al, 2013;Wang, Dong, et al, 2016;Wen et al, 2012), β-lactams (X. He et al, 2017;He, Wei, et al, 2019;Liu, Zhang, et al, 2016), and others (Dong et al, 2021;Li, Hou, et al, 2021;Xie et al, 2018) (Table 3).…”
Section: Veterinary Drug Residuesmentioning
confidence: 99%
“…In recent years, with the support of phage display that provides a powerful tool for the development of scFvs in veterinary drug immunoassays, scFvs against a variety of veterinary drugs have been screened, including β‐agonists (J. X. Dong et al., 2012; Dong, Xie, et al., 2014; Lee et al., 2018; Lu et al., 2020; H. Wang et al., 2010), fluoroquinolones (Tao et al., 2013; Wang, Dong, et al., 2016; Wen et al., 2012), β‐lactams (X. He et al., 2017; He, Wei, et al., 2019; Liu, Zhang, et al., 2016), and others (Dong et al., 2021; C. Li et al., 2016; Li, Hou, et al., 2021; Xie et al., 2018) (Table 3).…”
Section: Application Prospects Of Scfvs In Food Safetymentioning
confidence: 99%
“…To achieve better production of recombinant human collagen for various applications, it would be an optimized alternative strategy to express functional fragments of human collagen, according to the results described above, we synthesized a DNA fragment encoding a functional section of type I human collagen and then cloned it into a temperature-controlled expression vector, pBV220 which has been used over the past two decades for protein expression in E. coli [ 20 , 21 , 22 , 23 ].…”
Section: Introductionmentioning
confidence: 99%