Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources

Thavasi, R.; Jayalakshmi, S.; Balasubramanian, T.; Banat, Ibrahim M.

doi:10.1007/s11274-007-9609-y

Cited by 114 publications

(74 citation statements)

References 41 publications

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“…The most studied low-molecular-weight biosurfactant compounds are lipopeptides and glycolipids. Lipopeptides are mainly produced by members of the Bacillus species; they are composed of different families and each family is constituted of several variants, which can differ in their fatty acid chain and their peptide moiety (Dastgheib et al, 2008;Jacques, 2010;Thavasi et al, 2008Thavasi et al, , 2011.…”

Section: Low Molecular Weight Compounds 211 Lipopeptidesmentioning

confidence: 99%

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

Fracchia¹,

Cavallo²,

Martinotti³

et al. 2012

Biomedical Science, Engineering and Technology

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Section: Low Molecular Weight Compounds 211 Lipopeptidesmentioning

confidence: 99%

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

Fracchia¹,

Cavallo²,

Martinotti³

et al. 2012

Biomedical Science, Engineering and Technology

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“…Several renewable substrates from various sources, particularly from well-known industrial wastes have been intensively studied for microorganism cultivation and biosurfactant production on a laboratory scale (Daniel et al, 1998;Solaiman et al, 2004;Felse et al, 2007;Thavasi et al, 2008;Sitohy et al, 2010;Parekh et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Rashad¹,

Nooman²,

Ali³

et al. 2014

Grasas y Aceites

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SUMMARY:The production of sophorolipids by Candida bombicola NRRL Y-17069 grown in a mixture of sunflower oil cake and crude soybean oil as economic substrates with different fermentation techniques was studied. The highest yield (49.5 g·100 g −1 substrates) was obtained from solid state fermentation after employing a new concept for extraction by methanol (E I) followed by ethyl acetate (E II), then partially purified with hexane (E III). The course of time of fermentation was also studied, and E I extracted of the 12 th day showed the minimum surface tension (45 mN·m −1 ) at a critical micelle dilution (CMD) of 10% concentration. The produced sophorolipids were characterized and confirmed by FTIR and 1 H NMR spectroscopy. The anticancer activity of the produced compounds was assessed against MCF-7, HepG2, A549, HCT116 cancer cell lines and the results revealed that E III and E IV (a mixture of E I & E III) act as promising anticancer agents in HepG2 and A549 by inhibiting urokinase and histone deacetylase activities. KEYWORDS: Anticancer activity; Candida bombicola; Solid state fermentation; Sophorolipids; Soybean oil; Sunflower oilcakeRESUMEN: Producción, caracterización y actividad anticancerígena de soforolípidos producidos mediante fermentación en estado sólido con Candida bombicola de tortas de girasol y aceite de soja. Se estudió la producción de soforolípidos por Candida bombicola NRRL Y-17069 cultiva con diferentes técnicas de fermentación en una mezcla de torta de girasol y aceite de soja crudo, como sustratos económicos. El rendimiento más alto (49,5 g·100 g −1 de sustrato) se obtuvo por fermentación en estado sólido después de extraer con metanol (IE) seguido de acetato de etilo (EII), y de purificación parcial con hexano (EIII). También se estudió el tiempo de fermentación, considerando que el extracto IE de 12 días mostró una tensión superficial mínima (45 mN·m −1 ) a una dilución micelar crítica (CMD) de concentración 10 %. Los soforolípidos producidos se caracterizaron y se confirmaron mediante espectroscopia FTIR y RMN de 1 H. La actividad anticancerígena de los compuestos producidos se evaluó en células MCF-7, HepG2, A549, líneas celulares de cáncer de HCT116 y los resultados revelaron que EIII y EIV (una mezcla de EI y EIII) actúan como prometedores agentes anticancerígenos en HepG2 y A549 inhibiendo las actividades de uroquinasa e histona desacetilasa.

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“…Many published studies reported the successful isolation of biosurfactant producing organisms from soil polluted with hydrocarbons or mineral oils (Mukherjee, et al, 2009;Mulligan, 2005). We were successful in isolating bacteria with the ability to produce biosurfactants from the soil samples collected from garage oil contaminated soils like many other scientists have chosen the same source for soil collection, (Thavasi, et al, 2008 andSaravanan, et al, 2012).…”

Section: Screening Of Biosurfactant Producing Bacteria In Msm Medimentioning

confidence: 94%

Comparative Study of Biosurfactant Producing Organisms Isolated from Pesticide and Oil Contaminated Soil

Patil¹

2018

IJRASET

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Biosurfactants are diverse groups of surface active molecules synthesized by microorganisms which help in stabilizing emulsion, enhancing foaming and reducing surface tension. In this study, six soil samples were collected from pesticide and oil contaminated sites. Each sample was enriched in mineral salt medium then serially diluted and pour plated in the nutrient agar plates. A total of 32 morphologically distinct isolates were isolated in pure form from soil samples which were screened for the purpose of biosurfactant production. To confirm and compare the ability of isolates in biosurfactant production from different sources, different screening methods including foaming, emulsification index E24, drop collapse method and oil spreading test were assessed. Curd whey was used as a low cost growth medium for biosurfactant producing organisms. Kocuria rosea from pesticide contaminated soil and Micrococcus luteus/ lylae from oil contaminated soil sample were potent biosurfactant producers which were furthermore identified. Kocuria rosea showed highest emulsification index and prominent zone for oil spreading assay as compared to Micrococcus luteus/ lylae which suggested that biosurfactant producers isolated from pesticide contaminated soil sample have higher potency than biosurfactant producers isolated from oil contaminated soil sample.

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Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources

Cited by 114 publications

References 41 publications

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Comparative Study of Biosurfactant Producing Organisms Isolated from Pesticide and Oil Contaminated Soil

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