Production and characterization of a thermostable bioflocculant from Bacillus subtilis F9, isolated from wastewater sludge

Giri, Sib Sankar; Muthukumar, Harshiny; Sen, Shib Sankar; Sukumaran, V.; Park, Taesung

doi:10.1016/j.ecoenv.2015.06.010

Cited by 67 publications

(32 citation statements)

References 29 publications

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“…For genotypic identification, the bacterial 16S rDNA fragments in genomic DNA was amplified using universal 16S rDNA primers, forward primer 8F (5 0 AGA GTT TGA TCC TGG CTC AG-3 0 ) and reverse primer 1492R (5 0 CGG TTA CCT TGT TAC GAC TT-3 0 ). The amplified product was sequenced and submitted in the NCBI database (Giri, 2015). From the analysis, two isolates were belonged to Lysinibacillus sphaericus and another one belonged to Bacillus safensis.…”

Section: S Rdna Sequencingmentioning

confidence: 99%

Bioelectricity production from microbial fuel cell using mixed bacterial culture isolated from distillery wastewater

Samsudeen

Radhakrishnan

Matheswaran

2015

Bioresource Technology

101

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Section: S Rdna Sequencingmentioning

confidence: 99%

Bioelectricity production from microbial fuel cell using mixed bacterial culture isolated from distillery wastewater

Samsudeen

Radhakrishnan

Matheswaran

2015

Bioresource Technology

101

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“…Bioflocculant offers many advantages for suspended solids (SS) removal, such as high security and efficiency, low cost, being nontoxic, and producing no secondary pollution for the environment [4–10]. The use of bioflocculant for SS removal has been widely used in industrial, domestic, and building material and livestock wastewater treatment as a new water treatment agent [11–14]. Although there were some general reports of bioflocculant in wastewater treatment, relevant research and application of bioflocculant in aquaculture wastewater treatment have rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing BacteriumBacillus megateriumSP1 and Its Application in Aquaculture Wastewater Treatment

Luo

Zhao

Huang

et al. 2016

BioMed Research International

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A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L−1 of glucose and 0.5 g L−1 of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal culture conditions was applied into aquaculture wastewater treatment. The removal rates of chemical oxygen demand (COD), total ammonia nitrogen (TAN), and suspended solids (SS) in aquaculture wastewater reached 64, 63.61, and 83.8%, respectively. The volume of biofloc (FV) increased from 4.93 to 25.97 mL L−1. The addition of Bacillus megaterium SP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology.

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“…The culture temperature associates well with the metabolism of microorganisms in use (Giri et al 2015;Salehizadeh and Shojaosadati 2001). Figure 3b shows that fermentation at Fig.…”

Section: Effect Of Initial Ph Temperature Inoculum Size and Nitrogementioning

confidence: 99%

Palm oil mill effluent as a low-cost substrate for bioflocculant production by Bacillus marisflavi NA8

Nurul-Adela

Nasrin

Loh

2016

Bioresour. Bioprocess.

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Background: Palm oil mill effluent (POME) generated from the palm oil milling process contains high organic matter from which the molecule of interest for bioconversion such as fermentable sugar can be derived. This study examined the enzymatic hydrolysis of complex celluloses and polysaccharides present in POME with the aim to release the simple sugars as low-cost fermentable feedstock convertible into bioflocculant. Results:The maximum concentration of fermentable sugar produced after 24 h of hydrolysis was 30.5 g/L. The POME hydrolysate served as a carbon source for Bacillus marisflavi NA8 to produce bioflocculant. The optimum conditions obtained were an initial pH of 7.0 and a temperature of 37 °C with an inoculum size of 5 % (v/v), yielding 6.4 g/L or 32 kg/t bioflocculant from POME. Conclusion:This finding indicated that POME could be utilized as a low-cost substrate to improve the feasibility of commercial production of bioflocculant.

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Production and characterization of a thermostable bioflocculant from Bacillus subtilis F9, isolated from wastewater sludge

Cited by 67 publications

References 29 publications

Bioelectricity production from microbial fuel cell using mixed bacterial culture isolated from distillery wastewater

Bioelectricity production from microbial fuel cell using mixed bacterial culture isolated from distillery wastewater

Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing BacteriumBacillus megateriumSP1 and Its Application in Aquaculture Wastewater Treatment

Palm oil mill effluent as a low-cost substrate for bioflocculant production by Bacillus marisflavi NA8

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