Production and characterisation of monoclonal and polyclonal amtobpdoes agaomst bovine osteocalcin

Abstract

SUMMARY: Monoclonal and polycl0nal antibodies were produced against osteocalcin purified from bovine bone. The antibodies were used for the development of a two-site ELISA. A monoclonal antibody recognising residues 15-49 of bovine osteocatcin generated by cleavage with 70% formic acid was used as capture antibody and a biotinylated polyclonal antibody recognising the same peptide was used for detection. The two-site assay did not detect any tryptic peptides of bovine osteocalcin (residues 1-19, 20-43, and 45-49) nor did it detect the peptide 1-14 or the synthetic peptide 37-49. Cross-reaction was observed for osteocalcin in plasma from sheep, goat, chicken and human but not for osteocalcin from rat and pig.