Production and Biochemical Characterization of an Extracellular Lipase from Rhizopus chinensis CCTCC M201021

Pogori, Natascha; Cheikhyoussef, Ahmad; Xu, Yongping; Wang, Dong

doi:10.3923/biotech.2008.710.717

Cited by 18 publications

(12 citation statements)

References 23 publications

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“…Table 1 indicated that the lipase was activated by (0.02M, 0.08M) K þ , (0.08M, 0.2M) NH 4 þ , Ca 2þ and Mg 2þ in all concentrations used; especially, by 0.2M Ca 2þ its activity significantly increased to 63%, but it was not affected by 0.2M K þ and 0.02M NH 4 þ . These findings are consistent with those reported by Acikel et al, [14] Ma et al [17] and Pogori et al [15] SDS In the water/SDS system, the mole ratio v o is directly proportional to the size of the dispersed water pool. [16] However, Zn 2þ and Fe 3þ inhibited the activity of the lipase to some extents, suggesting that they were able to alter enzyme conformation.…”

Section: Effect Of Various Cations On the Activity Of Lipasesupporting

confidence: 92%

“…Depending on the concentration of metal ions, lipase activity increased or decreased (Table 1 and Figure 2). [15] Fe þ3 in all concentrations used has the most potent inhibitory effect on lipase activity. The effect of Ca 2þ has been suggested to be due to the formation of long-chain fatty acid calcium salts.…”

Section: Effect Of Various Cations On the Activity Of Lipasementioning

confidence: 95%

“…[14,15] Therefore, the effect of some metal ions on the lipase activity in dormant seeds was assessed. [14,15] Therefore, the effect of some metal ions on the lipase activity in dormant seeds was assessed.…”

Section: Effect Of Various Cations On the Activity Of Lipasementioning

confidence: 99%

See 2 more Smart Citations

Characteristics of lipase in dormant seeds catalysed hydrolysis of olive oil in SDS‐olive oil reversed microemulsions

El‐Hefnawy

Sakran

2014

Can J Chem Eng

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The activity of lipase-catalysed hydrolysis of olive oil in SDS-1-butanol-olive oil-water microemulsions was investigated. The kinetics of lipases in reverse microemulsions and water were also compared. The influences of the characteristic parameters of these systems, such as pH, temperature (T), molar ratio (v o ) of water to SDS, the concentrations of enzyme and substrate, on the activity of an enzyme were examined. According to the optimum conditions the production and activity were measured at pH ¼ 4, T ¼ 40 8C, v o ¼ 12. The activation energy of the reaction was calculated from the Arrhenius plot. It was found that the hydrolysis reaction obeyed Michaelis-Menten kinetics and the apparent Michaelis K m,a and the apparent maximal reaction rate V max were determined. The effect of microemulsion's surfactant (SDS) on enzymatic activity was also discussed.

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Section: Effect Of Various Cations On the Activity Of Lipasesupporting

confidence: 92%

Section: Effect Of Various Cations On the Activity Of Lipasementioning

confidence: 95%

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Characteristics of lipase in dormant seeds catalysed hydrolysis of olive oil in SDS‐olive oil reversed microemulsions

El‐Hefnawy

Sakran

2014

Can J Chem Eng

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“…The significance of each coefficient was determined by Student's t ‐test and p ‐values, which are listed in Table . Larger the magnitude of t ‐value and smaller p ‐value, more significant is the corresponding coefficient . This implies that the values of factors having ‘ p ’ values lesser than 0.05 are statistically more significant.…”

Section: Multiple Responses Optimization and Model Buildingmentioning

confidence: 96%

Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization

Potumarthi

Liu

Wan

et al. 2012

Asia-Pacific J Chem Eng

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A modified Response Surface Methodology was used for the optimization of lipase production by immobilized cells of Rhizopus oryzae (ATCC 96382). Multiple responses optimization strategy was adopted to optimize process condition of surface immobilization technique for the optimization of lipase productivity. Five parameters, pH, Temperature, revolutions per minute, olive oil and glucose were considered, and their effect was evaluated both qualitatively and quantitatively by using a modified central composite design approach and model validation. The second order regression equation was developed for the three yield parameters are YP/B, YL/P and YL/B, where Y is the yield parameter at their corresponding values of L: Lipase (U/mL), P: Protein (mg/mL) and B: Biomass (mg/mL). The regression equation was fitted and model fit (R2) was 0.604 (YP/B), 0.746 (YL/P) and 0.618 (YL/B), respectively showing an effective fit. Further, the model was validated theoretically and experimentally at higher level of production. Different growth patterns were observed during 120 h of fermentation period. © 2012 Curtin University of Technology and John Wiley & Sons, Ltd.

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“…These results indicate that the composition of vegetable seed oils (nature of the fatty acids constituting the acylglycerols) affected Botryosphaeria ribis EC-01 metabolism (Table 1). Pogori et al, (2008) investigated lipase production by Rhizopus chinensis CCTCC M201021 and showed that soybean oil enhanced lipase production and was highest among other oils studied. Comparatively, Botryosphaeria ribis EC-01 was previously shown to produce highest lipase titres on soybean oil and glycerol, while eight isolates of Botryosphaeria rhodina produced significantly lower enzyme titres (Messias, 2008).…”

Section: Effect Of Different Vegetable Oils As Sole Carbon Source Formentioning

confidence: 99%

Soybean Oil and Meal as Substrates for Lipase Production by Botryosphaeria ribis, and Soybean Oil to Enhance the Production of Botryosphaeran by Botryosphaeria rhodina

Barbosa¹,

Messias²,

Andrade³

et al. 2011

Soybean - Biochemistry, Chemistry and Physiology

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Production and Biochemical Characterization of an Extracellular Lipase from Rhizopus chinensis CCTCC M201021

Cited by 18 publications

References 23 publications

Characteristics of lipase in dormant seeds catalysed hydrolysis of olive oil in SDS‐olive oil reversed microemulsions

Characteristics of lipase in dormant seeds catalysed hydrolysis of olive oil in SDS‐olive oil reversed microemulsions

Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization

Soybean Oil and Meal as Substrates for Lipase Production by Botryosphaeria ribis, and Soybean Oil to Enhance the Production of Botryosphaeran by Botryosphaeria rhodina

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