Production and Bioactivity of Glucooligosaccharides and Glucosides Synthesized using Glucansucrases

Kim, Young‐Min; Kang, Hee-Kyoung; Moon, Yea Hwang; Nguyen, Thi Thanh Hanh; Day, Donal F.; Kim, Doman

doi:10.1002/9781118817360.ch10

Cited by 5 publications

(8 citation statements)

References 103 publications

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“…In this regard, the concept of prebiotics is gaining an ever wider realization in the practice of producing healthy foods [2,3]. are reduced, the production of short-chain fatty acids increases, and the overall health status of the organism is improved [4,6,7]. The mechanisms of prebiotic action of oligosaccharides are not fully understood, but their beneficial effects are probably a combination of their specific monosaccharide composition, type of linkages and metabolic pathways for consumption by probiotic bifidobacteria and lactobacilli in the lower intestinal tract of mammals.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, the populations of harmful bacteria such as Salmonella spp. Currently, the production of oligosaccharides is achieved by enzymatic hydrolysis of polysaccharides, or by synthesis using glycosyltransferase enzymes and an inexpensive and substrate such as sucrose [6,10]. are reduced, the production of short-chain fatty acids increases, and the overall health status of the organism is improved [4,6,7].…”

Section: Introductionmentioning

confidence: 99%

“…and Helicobacter spp. Glucansucrases of the glycoside hydrolase family 70 (GH70) produced by LAB of the genera Lactobacillus, Leuconostoc, Weissella and Streptococcus are of particular interest as they are capable of synthesizing short-chain GOSs with specific and defined linkages depending on the type of the used enzyme [6,11,12]. In order to quantify the effect of prebiotics on the composition of the microbial population of the intestinal tract have been developed in vitro approaches determining the prebiotic index (PI) and a measure of the prebiotic effect (MPE), which provide information on the quantitative and functional changes in the major microbial groups, due to the consumption of prebiotics [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…and Helicobacter spp. are reduced, the production of short-chain fatty acids increases, and the overall health status of the organism is improved [4,6,7]. In order to quantify the effect of prebiotics on the composition of the microbial population of the intestinal tract have been developed in vitro approaches determining the prebiotic index (PI) and a measure of the prebiotic effect (MPE), which provide information on the quantitative and functional changes in the major microbial groups, due to the consumption of prebiotics [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…By applying these concepts it is possible to evaluate the effectiveness of various prebiotics, among which the glucooligosaccharides has particularly high prebiotic value [8]. Currently, the production of oligosaccharides is achieved by enzymatic hydrolysis of polysaccharides, or by synthesis using glycosyltransferase enzymes and an inexpensive and substrate such as sucrose [6,10]. Glucansucrases of the glycoside hydrolase family 70 (GH70) produced by LAB of the genera Lactobacillus, Leuconostoc, Weissella and Streptococcus are of particular interest as they are capable of synthesizing short-chain GOSs with specific and defined linkages depending on the type of the used enzyme [6,11,12].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13–300 acceptor reactions with maltose, raffinose and lactose

Bivolarski

Vasileva

Gabriel³

et al. 2018

Engineering in Life Sciences

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In the present work, we report an efficient synthesis of glucooligosaccharides (GOSs) with prebiotic potential by novel glucansucrase URE 13–300 from Leuconostoc mesenteroides URE 13 strain. The highest total yield of GOSs with degree of polymerization (DP) from 3 to 6 was obtained with maltose as an acceptor and maltose/sucrose (M/S) ratio 1–136 g/L. An efficient modulation of GOSs composition is achieved by varying the M/S ratio. At M/S = 1, 2, 4 and 7 the content of DP3 products gradually increase from 54.50 to 91.70%. When the M/S ratio was decreased the synthesis of DP>3 GOSs is predominant and reaches 75.60% (M/S = 0.25). In addition, the maltose derived GOSs with DP>3, as well as raffinose and lactose glucosylation products have a branched structure which is prerequisite for increased prebiotic potential. The synthesized GOSs were efficiently metabolized by probiotic strains of Lb. plantarum S26, Lb. brevis S27 and Lb. sakei S16, and the calculated values of specific growth rate (μ) were nearly identical to this on glucose media, when maltose derived GOSs were used as a carbohydrate source. Strain specific features were observed in the utilization of the synthesized GOSs, as well as in the production of lactic acid and acetic acid.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13–300 acceptor reactions with maltose, raffinose and lactose

Bivolarski

Vasileva

Gabriel³

et al. 2018

Engineering in Life Sciences

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Synthesis of novel bioactive lactose‐derived oligosaccharides by microbial glycoside hydrolases

Díez-Municio

Herrero

Olano

et al. 2014

Microbial Biotechnology

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Prebiotic oligosaccharides are increasingly demanded within the Food Science domain because of the interesting healthy properties that these compounds may induce to the organism, thanks to their beneficial intestinal microbiota growth promotion ability. In this regard, the development of new efficient, convenient and affordable methods to obtain this class of compounds might expand even further their use as functional ingredients. This review presents an overview on the most recent interesting approaches to synthesize lactose-derived oligosaccharides with potential prebiotic activity paying special focus on the microbial glycoside hydrolases that can be effectively employed to obtain these prebiotic compounds. The most notable advantages of using lactose-derived carbohydrates such as lactosucrose, galactooligosaccharides from lactulose, lactulosucrose and 2-α-glucosyl-lactose are also described and commented.

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Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

García-González

Plou

Cervantes

et al. 2019

Microbial Biotechnology

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Summary Metschnikowia reukaufii is a widespread yeast able to grow in the plants’ floral nectaries, an environment of extreme conditions with sucrose concentrations exceeding 400 g l−1, which led us into the search for enzymatic activities involved in this sugar use/transformation. New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload‐sucrose reactions. These products were purified and structurally characterized by MS‐ESI and NMR techniques. The reaction mixture included new sugars showing a great variety of glycosidic bonds including α‐(1→1), α‐(1→3) and α‐(1→6) linkages. The main product synthesized was the trisaccharide isomelezitose, whose maximum concentration reached 81 g l−1, the highest amount reported for any unmodified enzyme or microbial extract. In addition, 51 g l−1 of the disaccharide trehalulose was also produced. Both sugars show potential nutraceutical and prebiotic properties. Interestingly, the sugar mixture obtained in the biosynthetic reactions also contained oligosaccharides such as esculose, a rare trisaccharide with no previous NMR structure elucidation, as well as erlose, melezitose and theanderose. All the sugars produced are naturally found in honey. These compounds are of biotechnological interest due to their potential food, cosmeceutical and pharmaceutical applications.

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Production and Bioactivity of Glucooligosaccharides and Glucosides Synthesized using Glucansucrases

Cited by 5 publications

References 103 publications

Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13–300 acceptor reactions with maltose, raffinose and lactose

Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13–300 acceptor reactions with maltose, raffinose and lactose

Synthesis of novel bioactive lactose‐derived oligosaccharides by microbial glycoside hydrolases

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

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