Producing Succinic Acid with Actinobacillus succinogenes: Optimizing the Composition of the Medium Using Plackett-Burman Design

Phuengjayaem, Sukanya; Teeradakorn, Siriluk

doi:10.12982/cmujns.2016.0019

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…According to Phuengjayaem et al (2016), the presence of inhibitors, such as 5hydroxymethylfurfural and furfural, might…”

Section: Screening Effect Of Solid Loading and Agitation Ratementioning

confidence: 99%

Biotechnological Biosuccinic acid (Bio-SA) production from green microalgae Chlorella sp. hydrolysate: Synergistic effect of agitation and substrate concentration assessment using RSM

Wan Zakaria,

Kassim,

Mohd Radzi

2024

ASEAN J. Chem. Eng.

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This paper evaluates the potential of Chlorella sp. microalgae biomass as a feedstock for biosuccinic acid (bio-SA) production via the fermentation process. In this study, the biomass was pretreated and hydrolyzed using a mild acid before being fermented with Actinobacillus succinogenes. The influence of the initial biomass concentration and agitation rate on bio-SA production during batch fermentation was evaluated using a Response Surface Methodology (RSM) design. The results of the study indicated that Chlorella sp. microalgae biomass contained various types of sugars, with glucose identified as the dominant reducing sugar in the Chlorella sp. hydrolysate. According to the RSM analysis, the study showed that changes in the initial biomass concentration and agitation rate could significantly affect bio-SA production from Chlorella sp. hydrolysate. The highest bio-SA concentration of 14.56 g/L with a yield of 0.62 g/g was achieved when fermentation was performed using 10% (w/v) biomass at 150 rpm. Therefore, this study suggests that Chlorella sp. hydrolysate can be an alternative and renewable feedstock for efficient bio-SA production.

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“…According to Phuengjayaem et al (2016), the presence of inhibitors, such as 5hydroxymethylfurfural and furfural, might…”

Section: Screening Effect Of Solid Loading and Agitation Ratementioning

confidence: 99%

Biotechnological Biosuccinic acid (Bio-SA) production from green microalgae Chlorella sp. hydrolysate: Synergistic effect of agitation and substrate concentration assessment using RSM

Wan Zakaria,

Kassim,

Mohd Radzi

2024

ASEAN J. Chem. Eng.

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“…The sugars in the resultant hydrolysate are then subjected to fermentation by succinic-acid-producing bacteria. Many renewable lignocellulose resources, such as sugar cane bagasse [ 3 ], corn fiber [ 4 ], corn stover [ 5 , 6 ] , cotton stalk [ 7 ], corn core, rice straw, wheat straw [ 8 ], and sorghum straw [ 9 ], have been used to generate hydrolysate, which is subsequently fermented by A. succinogenes . In addition to the aforementioned agriculture waste, grass-based biomass has considerable potential as lignocellulosic feedstock.…”

Section: Introductionmentioning

confidence: 99%

Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass

Lee

Lin

Lee

et al. 2022

Biotechnol Biofuels

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Background Napier grass biomass can be hydrolyzed mainly containing glucose and xylose after alkaline pretreatment and enzymatic hydrolysis. This biomass can be fermented using Actinobacillus succinogenes to produce succinic acid. The yield of succinic acid was 0.58 g/g. Because metabolizing xylose could produce more acetic acid, this yield of succinic acid was lower than that achieved using glucose as the sole carbon source. Results The addition of glycerol as a fermentation substrate to Napier grass hydrolysate increased the reducing power of the hydrolysate, which not only increased the production of succinic acid but also reduced the formation of undesirable acetic acid in bacterial cells. At a hydrolysate:glycerol ratio of 10:1, the succinic acid yield reached 0.65 g/g. The succinic acid yield increased to 0.88 g/g when a 1:1 ratio of hydrolysate:glycerol was used. For the recovery of succinic acid from the fermentation broth, an outside-in module of an ultrafiltration membrane was used to remove bacterial cells. Air sparging at the feed side with a flow rate of 3 L/min increased the filtration rate. When the air flow rate was increased from 0 to 3 L/min, the average filtration rate increased from 25.0 to 45.7 mL/min, which corresponds to an increase of 82.8%. The clarified fermentation broth was then electrodialized to separate succinate from other contaminated ions. After electrodialysis, the acid products were concentrated through water removal, decolorized through treatment with activated carbon, and precipitated to obtain a purified product. Conclusions The yield of succinic acid was increased by adding glycerol to the hydrolysate of Napier grass. The downstream processing consisting of ultrafiltration membrane separation and single-stage electrodialysis was effective for product separation and purification. An overall recovery yield of 74.7% ± 4.5% and a purity of 99.4% ± 0.1% were achieved for succinic acid.

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“…(5) (7) Where "n" = number of operating years, "CF" = cash flow, "A" = the last period with a negative cumulative cash flow, "B" = the absolute value of the cumulative cash flow at the end of period A and "C" = cash flow during the subsequent period. Table 3.7 details regarding the interested unit operations for equipment sizing [31], [85].…”

Section: Project Lifetime 15 Yearsmentioning

confidence: 99%

Techno-economic analysis of succinic acid production from sugarcane molasses

Rongsawad

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This study proposes an alternative approach to address global warming concerns by introducing bio-based succinic acid production derived from molasses and waste CO2�in ethanol production�as a substitute for conventional petroleum-based methods. The evaluation focuses on the downstream process; reactive extraction, aiming to assess its feasibility in terms of technical, economic, energy, and CO2�emission performance. The results indicate that the integrated process, Int. ETOH+SA 25:75, exhibits remarkable benefits, including a 66% reduction in CO2�emissions from ethanol production, a 77% decrease in energy consumption compared to petroleum-based methods, and NPV of 2,180 million USD with a 40.81% IRR and a 5-year payout period. Additionally, the implementation of a heat exchanger network reduces utility requirements by 39% and CO2�emissions by 63% which can be concluded that that�integrating ethanol and succinic acid production�offers significant environmental advantages, enhanced energy efficiency, and economic benefits.

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Producing Succinic Acid with Actinobacillus succinogenes: Optimizing the Composition of the Medium Using Plackett-Burman Design

Abstract: ABSTRACT

Cited by 4 publications

References 22 publications

Biotechnological Biosuccinic acid (Bio-SA) production from green microalgae Chlorella sp. hydrolysate: Synergistic effect of agitation and substrate concentration assessment using RSM

Biotechnological Biosuccinic acid (Bio-SA) production from green microalgae Chlorella sp. hydrolysate: Synergistic effect of agitation and substrate concentration assessment using RSM

Production of succinic acid through the fermentation of Actinobacillus succinogenes on the hydrolysate of Napier grass

Techno-economic analysis of succinic acid production from sugarcane molasses

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