Producing proteins in transgenic plants and animals

Larrick, James W.; Thomas, David William

doi:10.1016/s0958-1669(00)00236-6

Cited by 134 publications

(59 citation statements)

References 86 publications

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“…Recently, a transgenic plant has been widely used as an alternative for recombinant protein expression. Numerous proteins have been successfully expressed in various plant species (see reviews in [22][23][24][25]). Use of transgenic plants as a vehicle for protein expression has many benefits considering its cost-efficiency, scaling-up capacity, high-product quality, and low-contamination risks (see reviews in [26][27][28][29]).…”

Section: Introductionmentioning

confidence: 99%

Expression of biologically active human insulin‐like growth factor 1 in Arabidopsis thaliana seeds via oleosin fusion technology

et al. 2011

Biotech and App Biochem

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Novel protein expression in plant-based systems has become an important tool in producing and studying therapeutic proteins. Among many plant-based systems developed so far, oleosin fusion technology is one of the most cost-effective and convenient methods. In this study, an important therapeutic protein, human insulin-like growth factor 1 (hIGF-1), was expressed in Arabidopsis thaliana seeds via this technology. The plant bias codon usage-optimized hIGF-1 gene was fused to the C-terminal of A. thaliana 18.5 kDa oleosin gene, and the fusion gene driven by an oleosin promoter was transferred into A. thaliana ecotype Col-0. The accumulation of oleosin-hIGF-1 fusion protein in transgenic seeds was up to 0.75% of total seed protein (TSP) and the expression level of hIGF-1 was 0.17% of the TSP, which was eight times higher than previously reported using other plant-based hIGF-1 production systems. The biological activity of the hIGF-1 as an oleosin-hIGF-1 fusion protein in vitro was demonstrated by using human SH-SY5Y neuroblastoma cells.

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Section: Introductionmentioning

confidence: 99%

Expression of biologically active human insulin‐like growth factor 1 in Arabidopsis thaliana seeds via oleosin fusion technology

et al. 2011

Biotech and App Biochem

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“…Seven years is far longer than has been reported for other seed expression systems (Fiedler and Conrad, 1995;Stöger et al, 2000;Larrick and Thomas, 2001;Ramírez et al, 2001). Other vegetable cell organelle systems have been used for recombinant protein accumulation (Staub et al, 2000).…”

Section: Discussionmentioning

confidence: 81%

Short communication Correct targeting of proinsulin in protein storage vacuoles of transgenic soybean seeds

Cunha¹,

Araújo²,

Leite³

et al. 2010

Genet. Mol. Res.

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“…Tissue specific expression of recombinant proteins is more advantageous than generalized expression as it will be easier to regulate the gene expression in a tissue specific manner (Larrick and Thomas, 2001). For example, promoter elements derived from the casein and whey families have been used to direct expression and secretion of the recombinant proteins in the milk of cows, sheep and goats (Rudolph, 1999).…”

Section: Which Recombinant Proteins To Be Produced?mentioning

confidence: 99%