Producing aromatic amino acid from corn husk by using polyols as intermediates

Yuen, Vincent Fung Kin; Xiao, Youchang; Jun, Daniel Tan Zhi; Ma, Xiaoqiang; Zhou, Jie; Panda, Smaranika; Yan, Ning; Zhou, Kang

doi:10.1016/j.biomaterials.2022.121661

Cited by 6 publications

(4 citation statements)

References 49 publications

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“…Both cultures contained 1 g/L CSM at the start of the fermentation. The RNA extraction 22 was conducted during the early exponential growth phase (OD600 is around 0.5). The samples were sequenced using the Illumina PE150 technology.…”

Section: Transcriptome Analysismentioning

confidence: 99%

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Metabolic engineering of Escherichia coli to convert ethanol into L-tyrosine

LIANG,

ZHOU

2023

Preprint

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Utilizing ethanol derived from waste streams could establish shorter biosynthetic metabolic pathways to produce target metabolites, such as acetyl-CoA-derived chemicals. We have previously designed and constructed a two-step heterologous ethanol utilization pathway (EUP) in Escherichia coli that converts ethanol into acetyl-CoA derived compounds, including polyhydroxybutyrate (PHB) and prenol. In this study, we engineered E. coli to produce tyrosine efficiently by using ethanol as a major carbon source. Ethanol was converted into acetyl-CoA and then metabolized to generate biomass, provide energy, and produce tyrosine. The engineered strain produced 1.6 g/L tyrosine from 10 g/L ethanol. We hypothesized that the glyoxylate shunt was activated when ethanol was used as sole carbon source, and it was confirmed by transcriptome analysis. The key node gene aceA, encoding isocitrate lyase was upregulated 74-fold compared with the strain growing on glucose. To our knowledge, this is the first report of producing tyrosine from ethanol. The results support that ethanol has the potential to become a renewable feedstock to produce structurally complex chemicals.

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Section: Transcriptome Analysismentioning

confidence: 99%

“…22 µm lter (Chemikalie Pte Ltd) into HPLC vials. All samples were analyzed by HPLC (Shimadzu LC-10) using the Agilent ZORBAX Eclipse Plus C18 100 mm…”

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confidence: 99%

Metabolic engineering of Escherichia coli to convert ethanol into L-tyrosine

LIANG,

ZHOU

2023

Preprint

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“…Currently, there are a variety of uses for biomass in many industrial applications such as energy. 3,4…”

Section: Introductionmentioning

confidence: 99%

“…Currently, there are a variety of uses for biomass in many industrial applications such as energy. 3,4 Biomass-derived biodiesel has become increasingly popular because of the advantages it offers. Not only is biodiesel renewable, but it is also biodegradable, environmentally benign, and non-toxic; hence, it is a good substitute for diesel fuel.…”

Section: Introductionmentioning

confidence: 99%

Determination of active sites on the synthesis of novel Lewis acidic deep eutectic solvent catalysts and kinetic studies in microalgal biodiesel production

et al. 2023

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Valorization of Agriculture Residues into Value‐Added Products: A Comprehensive Review of Recent Studies

Hoang,

Van Anh,

Yusuf

et al. 2024

The Chemical Record

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Global agricultural by‐products usually go to waste, especially in developing countries where agricultural products are usually exported as raw products. Such waste streams, once converted to “value‐added” products could be an additional source of revenue while simultaneously having positive impacts on the socio‐economic well‐being of local people. We highlight the utilization of thermochemical techniques to activate and convert agricultural waste streams such as rice and straw husk, coconut fiber, coffee wastes, and okara power wastes commonly found in the world into porous activated carbons and biofuels. Such activated carbons are suitable for various applications in environmental remediation, climate mitigation, energy storage, and conversions such as batteries and supercapacitors, in improving crop productivity and producing useful biofuels.

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Producing aromatic amino acid from corn husk by using polyols as intermediates

Cited by 6 publications

References 49 publications

Metabolic engineering of Escherichia coli to convert ethanol into L-tyrosine

Metabolic engineering of Escherichia coli to convert ethanol into L-tyrosine

Determination of active sites on the synthesis of novel Lewis acidic deep eutectic solvent catalysts and kinetic studies in microalgal biodiesel production

Valorization of Agriculture Residues into Value‐Added Products: A Comprehensive Review of Recent Studies

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