Producing amino acid fertilizer by hydrolysis of the fermented mash of food waste with the synergy of three proteases expressed by engineered Candida utilis

Sun, Tao; Xiao, Wenjuan; Jiang, Cuifeng; Wang, Jue; Liu, Zehuan

doi:10.1016/j.biteb.2019.100268

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…The selection of the EFW method is based on its recognized reputation as an environmentally friendly approach capable of efficiently extracting high-value fish protein hydrolysates. This process operates at low temperatures, utilizing biologically active protein molecules with strong enzymatic activity [11]. The importance of this approach to FW valorization is further demonstrated by the abundance of literature in the area [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the technical and economic viability of upcycling sustainable fish waste for bioproduct production

Okoro,

Nei,

Denayer

et al. 2024

Preprint

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The valorization of fish waste presents a promising avenue for sustainable resource utilization and economic value creation. In this regard, this paper conducted a comprehensive technoeconomic investigation into alternative technologies for the valorization of fish waste. Various technologies, including subcritical water hydrolysis, enzymatic hydrolysis, and anaerobic digestion, were considered in the analysis. Through economic modelling and sensitivity analyses using ASPEN Plus software, the feasibility and economic viability of different valorization strategies, designated as scenarios (a), (b), and (c) for the production of protein hydrolysate, electricity, protein hydrolysate, fatty acids, and electricity only, respectively, were assessed. The results revealed that utilizing subcritical water technology in scenario (b) yields the most economically viable strategy, with its positive Net Present Value (NPVs) of US$31.67 million indicating economic feasibility. Conversely, the NPVs of scenarios (a) and (c) of -US$24.95 million and of -US$12.09 million respectively demonstrated economic infeasibility. Further investigations into the economic viability demonstrated the important role of processing capacities and operating costs on economic feasibility. Despite challenges and uncertainties, the study underscores the potential for sustainable and economically viable utilization of fish waste, offering valuable insights for decision-makers in the seafood industry.

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Section: Introductionmentioning

confidence: 99%

Analysis of the technical and economic viability of upcycling sustainable fish waste for bioproduct production

Okoro,

Nei,

Denayer

et al. 2024

Preprint

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“…Accordingly, research on the enzymatic hydrolysis of Livestock blood has primarily focused on its application as a raw material in food additives and pharmaceutical products [11][12][13][14]. While research has been consistently conducted on various types of proteases to produce amino acid fertilizers from different organic waste materials, such as fishery [15] and food waste [16], studies on the production of amino acid fertilizers using Livestock blood as the raw material have not yet been published.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Enzymatic Degradability of Livestock Blood Pretreated with Ultrasonic Technique

Jeon,

Jeon

2024

Applied Sciences

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Livestock blood, a major organic waste generated by the livestock industry, poses a risk of pollution due to its rapid decomposition. However, it is a potential protein source for agricultural purposes due to its protein-rich organic matter. In this study, we conducted studies on an eco-friendly, scalable, and effective protein degradation process using livestock blood to reduce waste and produce an amino acid liquid fertilizer that can be recycled for agricultural use. Ultrasonic technology was applied as a pretreatment method to improve the enzymatic hydrolysis efficiency of proteins in livestock blood, and the optimal conditions that led to 95.91% solubilization rate of hemoglobin were ultrasound duration for 30 min at an ultrasound density of 0.5 W/mL. As a result of hydrolyzing ultrasonically pretreated blood by mixing exo- and endo-type proteolytic enzymes, the optimal combination was a mixture of Savinase® 1% and Flavourzyme® 1%. After 4 h of reaction, the protein concentration was 27.8 mg/mL and the amino acid concentration was confirmed to be 54.6 mg/mL. This is about 4.2 times higher than the amino acid concentration of blood without ultrasound pretreatment, 13.1 mg/mL, and it was confirmed that sonication has a significant effect on improving protein degradation efficiency. As protein degradation increased, the viscosity of blood gradually decreased, suggesting that the physical force applied to the agitator torque diminished during the enzyme reaction; a significant correlation between protein and amino acid concentrations (biological factors) and torque (mechanical factor) was observed. Measuring torque during an enzyme reaction can confirm the extent of the enzyme reaction, so it can be used as an indicator of reaction progress when scaling up the process in the future.

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“…based on the differences in their catalytic mechanisms 1 . Carboxypeptidases are commonly used in the food industry to produce amino acids 2 , prepare oligopeptides 3 , 4 , debitter protein hydrolysates and enhance flavor 5 , 6 . Some special carboxypeptidases are also used to cleave specific polypeptides 7 and amino acid sequences within polypeptides 8 , 9 in the field of biotechnology.…”

Section: Introductionmentioning

confidence: 99%

A new carboxypeptidase from Aspergillus niger with good thermostability, pH stability and broad substrate specificity

Song

Zhang

et al. 2021

Sci Rep

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A new serine carboxypeptidase gene, capA, was identified in Aspergillus niger CBS 513.88 by reading genomic information and performing sequence alignment, and the gene was cloned and expressed in Pichia pastoris GS115. In a shake flask, the enzyme activity of the recombinant strain GS115 (pPIC9K-capA) reached 209.3 U mg−1. The optimal temperature and pH for enzyme activity were determined to be 45 °C and 6.0, respectively. After incubation at 40–50 °C or at pH 4.0–8.0 for 1 h, the enzyme retained more than 80% or 60% of its initial activity. The presence of 1–10 mmol L−1 Mg2+ enhanced the activity of CapA, whereas 1–10 mmol L−1 Cu2+, Fe2+, or Co2+, 10 mmol L−1 Mn2+, or 1–10 mmol L−1 phenylmethylsulfonyl fluoride (PMSF) significantly inhibited its activity. CapA had a broad substrate specificity and preferred the hydrophobic amino acids Leu and Lys at the C terminus of proteins, and N-benzyloxycarbonyl-l-phenylalanyl-l-leucine (Cbz-Phe-Leu) was the optimal substrate, for which CapA exhibited Km 0.063 mmol L−1 and kcat/Km 186.35 mmol L−1 s−1. The good thermostability, pH stability and hydrolysis characteristics of CapA provide a solid foundation for application in the food and biotechnology fields.

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Producing amino acid fertilizer by hydrolysis of the fermented mash of food waste with the synergy of three proteases expressed by engineered Candida utilis

Cited by 5 publications

References 24 publications

Analysis of the technical and economic viability of upcycling sustainable fish waste for bioproduct production

Analysis of the technical and economic viability of upcycling sustainable fish waste for bioproduct production

Enhanced Enzymatic Degradability of Livestock Blood Pretreated with Ultrasonic Technique

A new carboxypeptidase from Aspergillus niger with good thermostability, pH stability and broad substrate specificity

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