Produção de metabólitos secundários por meio da cultura de tecidos vegetais

Souza, Julio Cezar de; Rescarolli, Cristine Luciana de Souza; Núñez, Cecília Veronica

doi:10.17648/2446-4775.2018.550

Cited by 5 publications

(3 citation statements)

References 32 publications

(33 reference statements)

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“…Among the auxins, 2,4-D at lower concentrations has been shown to be efficient for the induction of friable callus of Caesalpinia echinata (Fabaceae) [32]. Souza et al [33] reported that concentrations and dilutions of culture media and growth regulators have profound effects on the morphogenetic response of the explant at different stages of in vitro culture. On the other hand, growth regulators can act and control the processes of development of plant cells and tissues in culture media, as well as cell division and differentiation, organogenesis, dormancy and biosynthesis of secondary metabolites [34].…”

Section: Friable Callus Inductionmentioning

confidence: 99%

In vitro Regeneration and Callogenesis of Libidibia ferrea

Silva

Imakawa

Guedes³

et al. 2020

JEAI

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Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

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Section: Friable Callus Inductionmentioning

confidence: 99%

In vitro Regeneration and Callogenesis of Libidibia ferrea

Silva

Imakawa

Guedes³

et al. 2020

JEAI

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“…Os metabólitos secundários estão sendo obtidos graças as pesquisas que envolvem microrganismos, os quais elucidam a estrutura e ação biológica de uma gama de compostos de estruturas complexas e variada atividade biológica (SOUZA; RESCAROLLI; NUNEZ, 2018). São diversos os metabólitos secundários de origem microbiana responsáveis por estimular o desenvolvimento vegetal, substâncias da classe dos sideróforos, os quais são quelantes com massa molecular entre 400 e 2000 Da, têm função na ligação com o Fe (III), em condições de baixo ferro livre.…”

Section: Introductionunclassified

Substâncias Fitoestimulantes Produzidas Por Cepas De Streptomyces Spp. Isolados Da Rizosfera De Aniba Parviflora Syn A. Fragans (Macacaporanga)

Ferreira¹,

Júnior²,

Júnior³

et al. 2023

Biotecnologia Microbiana - Volume 1

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Objetivo: Este trabalho teve como objetivo avaliar a produção de substâncias fitoestimulantes por cepas de actinobactérias in vitro, isoladas da rizosfera na Amazônia. Método: Foram analisadas 10 cepas de actinobactérias (Streptomyces spp.), pertencentes ao acervo da Bacterioteca do Laboratório de Microbiologia da UFOPA, obtidos de solo rizosférico de Aniba parviflora Syn. A. fragans (Macacaporanga), em área de transição entre floresta ombrófila densa e Savana no município de Santarém-PA. Para a confirmação do gênero Streptomyces spp. foram avaliados morfológicos e moleculares pelo gene 16S rRNA, sendo assim nomeadas MPO1, MPO2, MPO3, MPO4, MPO5, MPO6, MPO7, MPO8, MP10, MPO11. Os morfotipos foram avaliadas quanto a capacidade de produção dos fitoestimulantes ácido indolacético, sideróforos, amônia, produção de fosfatases e solubilização de fosfato. Para tanto, foram realizados ensaios biológicos qualitativos, todos em triplicata. Resultados: Este estudo apresenta pela primeira vez a capacidade de actinobactérias do gênero Streptomyces spp. nativas da rizosfera de Aniba parviflora na Amazônia em produzir substâncias fitoestimulantes. Os isolados com melhor desempenho foram Streptomyces spp. MPO11 para produção sideróforos e AIA; Streptomyces spp. MPO1, Streptomyces spp. MPO2, Streptomyces spp. MPO11 na solubilização de fosfato, e Streptomyces spp. MPO6, Streptomyces sp. MPO7 na produção de amônia (NH3); e Streptomyces spp. MPO1 e Streptomyces spp. MPO10 na produção de fosfatases. Conclusão: As cepas de actinobactérias produzem substâncias fitoestimulantes, com destaque para Streptomyces spp. MPO 11, produtora de sideróforos, ácido indol-3-acético e solubilização de fosfato, apontando potencial uso na indução do crescimento vegetal.

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“…In plants, these compounds are present in small quantities and exert important biological activities for human health, being fundamental in the process of discovery and development of new drugs (Velu, Palanichamy, & Rajan, 2018). It is estimated that 35% of therapeutic medicines are of natural origin (Calixto, 2019), therefore, biotechnological techniques, such as in vitro plant cultivation, aim to increase the bioprospection of these compounds, by preventing the loss of biodiversity through the use of few natural sources, decreasing unsustainable extractivism and contributing to species conservation (Souza, Rescarolli, & Nunez, 2018).…”

Section: Introductionmentioning

confidence: 99%

Limonoid detection and profile in callus culture of sweet orange

Pessoa

Santos

et al. 2021

Acta Sci. Biol. Sci.

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Plant tissue culture has emerged as an important tool to produce bioactive compounds from various plant species, including the sustainable production of limonoids that are receiving considerable attention due to the benefits associated with human health such as anticancer activities. The purpose of the present study was to evaluate the capacity of limonoids aglycone production from callus culture from sweet orange cv. Pera (Citrus sinensis) seeds and identify the compounds produced in this cell line. Callus induction occurred in Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic (2,4-D), malt extract, agar and coconut water. For the analysis and identification of the limonoids, CG-MS-EI ion-positive mode and UPLC-QTOF-ESI were used operating in positive and negative mode. An intense peak corresponding to limonin appeared in the callus extracts at a retention time of 58.1 min. in CG-MS-EI and four major limonoids aglycone by positive ion mode UPLC-QTOF-ESI: limonin, nomilin, deacetylnomilin, and nomilinic acid. The culture medium was efficient at the bioproduction of limonoids aglycone in callus cultures of C. sinensis seeds. Therefore, data obtained from UPLC-QTOF-ESI proved its importance at identifying new compounds that benefit human health, and may assist future work in the identification of known or new limonoids in Citrus species and related genera.

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Produção de metabólitos secundários por meio da cultura de tecidos vegetais

Cited by 5 publications

References 32 publications

In vitro Regeneration and Callogenesis of Libidibia ferrea

In vitro Regeneration and Callogenesis of Libidibia ferrea

Substâncias Fitoestimulantes Produzidas Por Cepas De Streptomyces Spp. Isolados Da Rizosfera De Aniba Parviflora Syn A. Fragans (Macacaporanga)

Limonoid detection and profile in callus culture of sweet orange

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