Prodigiosin Production by Serratia marcescens UCP 1549 Using Renewable-Resources as a Low Cost Substrate

Abstract

A new strain of Serratia marcescens UCP1459 isolated from a semi-arid soil produced the natural red pigment prodigiosin, characterized by an uncommon pyrrolylpyrromethane skeleton. Prodigiosin is a promising drug due to its reported antifungal, immunosuppressive and anti-proliferative activities. The objective of this work was to indentify a suitable medium to simultaneously enhance S. marcescens growth and pigment production using renewable resources obtained from industrial wastes. S. marcescens produced the highest level of prodigiosin (49.5 g/L) at 48 h of cultivation using 6% “manipueira” (cassava wastewater) supplemented with mannitol (2%) at pH 7 and 28 °C. Carbohydrates in “manipueira” and mannitol play a role in the enhanced cell growth and prodigiosin production. The purified pigment extracted from the biomass was analyzed by mass spectrophotometry and showed the expected molecular weight of 324 Da corresponding to prodigiosin. In conclusion, we have successfully designed a new, economically feasible medium supporting enhanced S. marcescens growth and a high yield production of prodigiosin.

Optimization of Bioreactor Cultivation Parameters by Taguchi Orthogonal Array Design for Enhanced Prodigiosin Production

“…The molecular weight of the pigment was determined by using mass spectrometry which corresponding to 323.1 Da as shown in Figure S4 which agreed with the result obtained by [14].…”

“…The molecular weight of the pigment was determined by using mass spectrometry by using model DSQ [14]. 7 …”

Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species

“…On the other hand, our results show that, the supplementation of 20 % DPW broth with 1 % mannitol was more efficient in inducing prodigiosin production in P1 and fusant NRCF69 to (14.0 and 47.0 g L -1 , respectively). Casullo de Araújo et al (2010) recommended renewable resources obtained from industrial wastes (cassava wastewater) supplemented with mannitol to support the highest level of prodigiosin production (49.5 g L -1 ) using S. marcescens. Whereas Solé et al (1997) reported that glucose, maltose and other metabolizable sugar caused a reduction in prodigiosin due to catabolite repression or a decrease in pH observed in the cell suspensions, mannitol in the cultivation medium was a decisive factor in maintaining the pH at value of 7.0.…”

The Ecological Coherence of Temperature and Salinity Tolerance Interaction and Pigmentation in a Non-marine Vibrio Isolated from Salar de Atacama

“…For instance, Starič et al (2010) reported that V. ruber DSM 14379 did not produce pigmentation at low (<15°C) and high temperatures (>43°C). Casullo de Araújo et al (2010) reported that high temperatures (over 30°C) affected the prodigiosin production in S. marcescens UCP 1549 , and the ideal temperature for pigment production was 28°C. They reported that the activity of one or more enzymes involved in prodigiosin synthesis is affected at high temperatures (Casullo de Araújo et al, 2010).…”

“…Moreover, some other research groups reported that NaCl is required for growth and pigmentation in Serratia marcescens (Silverman and Munoz, 1973; Allen et al, 1983). Besides the impact of ion concentrations, the effect of the temperature on the activation of enzymes involved in prodigiosin biosynthetic pathway (Williams et al, 1971; Casullo de Araújo et al, 2010; Starič et al, 2010) has also been reported. However, most of the studies were related to room temperature or optimal temperature.…”