Prodigiosin-loaded electrospun nanofibers scaffold for localized treatment of triple negative breast cancer

Akpan, Udom M.; Pellegrini, Michael; Obayemi, John D.; Ezenwafor, Theresa; Browl, D.; Ani, C.J.; Yiporo, D.; Salifu, Ali A.; Dozie-Nwachukwu, S.; Odusanya, S.; Freeman, J. Reuben; Soboyejo, Winston O.

doi:10.1016/j.msec.2020.110976

Cited by 33 publications

(29 citation statements)

References 35 publications

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“…This indicates that the sustained release of the AME extract was effective in killing breast cancer cells or slowing down their proliferation. These observations are consistent with prior studies by Dozie-Nwachukwu et al 43 and Akpan et al 14 who reported the effects of the sustained release of prodigiosin from chitosan microspheres and electrospun PLGA/Ge scaffolds, respectively, on the reduction in the viabilities of breast cancer cells. However, when pluronic F127 was incorporated into the AME-loaded scaffolds (PLGA/Ge-F127/AME), the increased AME loading capacity coupled with the slowdown of AME release from the scaffolds elicited a higher percentage of apoptotic cells (in both cell lines) than the direct AME addition and the PLGA/Ge-AME scaffolds.…”

Section: Cytotoxic Effects Of the Released Ame Extract On Breast Cancer Cellssupporting

confidence: 93%

“…24 PLGA/Ge (3:1 w/w) was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) at a concentration (16% w/v) based on our prior work. 14 The prepared pluronic F127/AME, F127, and AME solutions were blended separately with the PLGA/Ge solution. All the blends were vortexed for 30 mins before loading into a syringe for electrospinning.…”

Section: Fabrication Of Ame-loaded Electrospun Fiber Scaffoldsmentioning

confidence: 99%

“…This outcome is consistent with our prior studies that reported a pluronic F127-mediated two-fold increase in prodigiosin's loading capacity in electrospun PLGA/Ge fibers. 14 Pluronic F127 is an amphiphilic, triblock copolymer, non-ionic surfactant that has been used to improve the interactions between biological membranes and lipophilic and hydrophilic substrates. 25 It increases the drug loading capacity especially at higher concentrations (above its critical micellar concentration in fibers) 29 through the self-assembly of the triblock copolymer molecules into micelles in aqueous solutions that encapsulate more drugs.…”

Section: In Vitro Ame Extract Release Profile and Kineticsmentioning

confidence: 99%

“…13 Prior works have also explored the development of sustainedrelease drug delivery systems in an attempt to maintain and localize the anticancer drug concentrations at desirable doses over longer durations than bulk systemic chemotherapy to maximize therapeutic effect. [14][15][16] A few researchers have reported the successful loading of AME extracts into drug delivery carriers to potentiate their cytotoxic effects through their sustained release to the target cells. [17][18][19] For instance, Aruan et al 17 incorporated AME leaf extracts into electrospun polyvinyl alcohol nanofiber scaffolds and demonstrated the cytotoxic effects of the released extracts on the growth of Staphylococcus aureus.…”

Section: Introductionmentioning

confidence: 99%

“…We also explored the incorporation of pluronic F127 into the AME-loaded scaffolds to increase the loading capacity of the AME and prolong its sustained release to maximize its antiproliferative effects. This is based on the outcome of our recent work, 14 which demonstrated that pluronic F127 increased prodigiosin's drug loading capacity in electrospun fibers, reduced the burst release, and prolonged sustained drug release.…”

Section: Introductionmentioning

confidence: 99%

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In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

et al. 2021

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This paper presents in vitro studies of the sustained release of Annona muricata leaf extracts (AME) from hybrid electrospun fibers for breast cancer treatment.Electrospun hybrid scaffolds were fabricated from crude AME extracts, poly(lacticco-glycolic acid)/gelatin (PLGA/Ge) and pluronic F127. The physicochemical properties of the AME extract and scaffolds were studied. The antiproliferative effects of the scaffolds were also assessed on breast cancer (MCF-7 and MDA-MB-231) and non-tumorigenic breast (MCF10A) cell lines. Scanning electron microscope micrographs revealed a random network of micro-and submicron fibers. In vitro drug release profiles, governed by quasi-Fickian diffusion at pH 7.

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Section: Cytotoxic Effects Of the Released Ame Extract On Breast Cancer Cellssupporting

confidence: 93%

Section: Fabrication Of Ame-loaded Electrospun Fiber Scaffoldsmentioning

confidence: 99%

Section: In Vitro Ame Extract Release Profile and Kineticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

et al. 2021

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Drug Delivery Systems for Photodynamic Therapy: The Potentiality and Versatility of Electrospun Nanofibers

Costa

Fangueiro

Ferreira

2022

Macromolecular Bioscience

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Recently, photodynamic therapy (PDT) has become a promising approach for the treatment of a broad range of diseases, including oncological and infectious diseases. This minimally invasive and localized therapy is based on the production of reactive oxygen species able to destroy cancer cells and inactivate pathogens by combining the use of photosensitizers (PSs), light, and molecular oxygen. To overcome the drawbacks of drug systemic administration, drug delivery systems (DDS) can be used to carrier the PSs, allowing higher therapeutic efficacy and minimal toxicological effects. Polymeric nanofibers produced by electrospinning emerged as powerful platforms for drug delivery applications. Electrospun nanofibers exhibit outstanding characteristics, such as large surface‐area‐to‐volume ratio associated with high drug loading, high porosity, flexibility, ability to incorporate and release a wide variety of therapeutic agents, biocompatibility, and biodegradability. Due to the versatility of this technique, fibers with different morphologies and functionalities, including drug release profile can be produced. The possibility of scalability makes electrospinning even more attractive for the development of DDS. This review aims to explore and show an up to date of the huge potential of electrospun nanofibers as DDS for different PDT applications and discuss the opportunities and challenges in this field.

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Recent advances in scaffold based electrospun for breast cancer research

Ebrahimbaygi,

Khazaei,

Valadbeigi

et al. 2024

Polymers for Advanced Techs

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One of the biggest challenges facing public health in the modern era is the management of cancer, a global health issue. Breast cancer (BC) is the most common malignancy among women worldwide. Among the most popular cancer treatment modalities are radiation, chemotherapy, and surgery. Chemotherapy, however, is regarded as the primary treatment option for cancer that has progressed to the final and metastatic stages. However, because of things like toxicity to healthy cells, poor drug absorption, trouble getting drugs to target tumor sites, and low therapeutic efficacy, traditional chemotherapy approaches are frequently insufficient. Nanotechnology offers the potential to overcome some of these limitations by creating new materials with unique properties through electrospinning. A straightforward and reasonably priced technique for creating biomaterials that can replicate the topography and structure of the cellular matrix is electrospinning. These materials have a large surface area, can be mechanically controlled, and have a level of fibers that can be adjusted from micrometers to nanometers. This review article emphasizes the potential of electrospun scaffolds for the treatment of BC while also offering a basic understanding of the procedure and nanostructured fibrous materials. This article reviews the most recent emerging electrospinning techniques in BC therapy. First, it briefly introduces the progress made in electrospinning in BC research over the past few years. Next, it investigates electrospinning by summarizing the techniques and materials used in the process. In addition, it demonstrates how crucial electrospinning three‐dimensional (3D) models are for the diagnosis and treatment of BC. This article also clarifies numerous electrospinning uses in several fields, such as cell culture, drug delivery, drug loading, and gene therapy. The final section discusses the advantages, limitations, and challenges electrospinning is willing to encounter in BC research.

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Prodigiosin-loaded electrospun nanofibers scaffold for localized treatment of triple negative breast cancer

Cited by 33 publications

References 35 publications

In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

Drug Delivery Systems for Photodynamic Therapy: The Potentiality and Versatility of Electrospun Nanofibers

Recent advances in scaffold based electrospun for breast cancer research

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