Prodiamesa olivacea: de novo biomarker genes in a potential sentinel organism for ecotoxicity studies in natural scenarios

Llorente, Lola; Herrero, Óscar; Aquilino, Mónica; Planelló, Rosario

doi:10.1016/j.aquatox.2020.105593

Cited by 5 publications

(4 citation statements)

References 65 publications

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“…The results of our pre-processing step show that RNA contamination from food sources, organisms’ microbiomes, and even experimental manipulation must be considered to limit artifacts of transcriptome assembly of novel species. To our knowledge, this has not been done for most environmental species (Caputo et al, 2020; Cogne et al, 2019; Llorente et al, 2020). In parallel, these results show the interest of considering RNA contaminants to reconstruct the holotranscriptome (host transcriptome and microbiota transcriptome) of G. fossarum , similarly to recent hologenome approaches performed in Daphnia magna or the metaproteomic studies recently tested in gammarids (Chaturvedi et al, 2023, Gouveia et al 2020).…”

Section: Resultsmentioning

confidence: 99%

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Koenig,

Baa-Puyoulet,

Lafont

et al. 2024

Preprint

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Metabolic pathways are targets of environmental contaminants underlying a large variability of toxic effects throughout biodiversity. However, the systematic reconstruction of metabolic pathways remains limited in environmental sentinel species due to the lack of available genomic data in many taxa of animal diversity. In order to improve the knowledge of the metabolism of sentinel species, in this study we used a multi-omics approach to reconstruct the most comprehensive map of metabolic pathways for a crustacean model in biomonitoring, the amphipod Gammarus fossarum. We revisited the assembly of RNA-seq data by de novo approaches drastically reducing RNA contaminants and transcript redundancy. We also acquired extensive mass spectrometry shotgun proteomic data on several organs from G. fossarum males and females to identify organ-specific metabolic profiles. The G. fossarum metabolic pathway reconstruction (available through the metabolic database GamfoCyc) was performed by adapting the genomic tool CycADS and we identified 377 pathways representing 7,630 annotated enzymes, 2,610 enzymatic reactions and the expression of 858 enzymes was experimentally validated by proteomics. Our analysis shows organ-specific metabolic profiles, such as an elevated abundance in enzymes involved in ATP biosynthesis and fatty acid beta-oxidation indicative of the high-energy requirement of the gills, or the key anabolic and detoxification role of the hepatopancreatic caeca, as exemplified by the specific expression of the retinoid biosynthetic pathways and glutathione synthesis. In conclusion, the multi-omics data integration performed in this study provides new resources to investigate metabolic processes in crustacean amphipods and their role in mediating the effects of environmental contaminant exposures in sentinel species.

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Section: Resultsmentioning

confidence: 99%

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Koenig,

Baa-Puyoulet,

Lafont

et al. 2024

Preprint

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“…Significant elevation of transcripts coding for CYP4g15 was also reported in the mosquito Aedes aegypti that was deemed resistant to multiple insecticides including permethrin and malathion [ 23 ]. Modulation of CYP4g15 was also observed in the aquatic dipteran Prodiamesa olivacea following exposure to various xenobiotics [ 24 ]. These studies, combined with the current data, support the potential significance of CYP4g15 in response to insecticides.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Insecticide Response-Associated Transcripts in the Colorado Potato Beetle: Relevance of Selected Cytochrome P450s and Clothianidin

Bouafoura

Bastarache

Ouédraogo

et al. 2022

Insects

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The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is known for its capacity to cause significant damages to potato crops worldwide. Multiple approaches have been considered to limit its spread including the use of a diverse arsenal of insecticides. Unfortunately, this insect frequently develops resistance towards these compounds. Investigating the molecular bases underlying the response of L. decemlineata against insecticides is of strong interest to ultimately devise novel and targeted approaches aimed at this pest. This work aimed to characterize, via qRT-PCR, the expression status of targets with relevance to insecticide response, including ones coding for cytochrome P450s, glutathione s-transferases, and cuticular proteins, in L. decemlineata exposed to four insecticides; chlorantraniliprole, clothianidin, imidacloprid, and spinosad. Modulation of levels associated with transcripts coding for selected cytochrome P450s was reported in insects treated with three of the four insecticides studied. Clothianidin treatment yielded the most variations in transcript levels, leading to significant changes in transcripts coding for CYP4c1, CYP4g15, CYP6a13, CYP9e2, GST, and GST-1-Like. Injection of dsRNA targeting CYP4c1 and CYP9e2 was associated with a substantial decrease in expression levels and was, in the case of the latter target, linked to a greater susceptibility of L. decemlineata towards this neonicotinoid, supporting a potential role for this target in clothianidin response. Overall, this data further highlights the differential expression of transcripts with potential relevance in insecticide response, as well as generating specific targets that warrant investigation as novel dsRNA-based approaches are developed against this insect pest.

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“…The changes affect the ecdysone response as well as the expression of insulin-like peptide. Other pathways are also affected by phthalates, such as oxidative stress and detoxification routes 44 and the stress response 14 . Finally, in C .…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to DEP and DEHP, BBP showed a marked effect: it increased the mRNA levels of almost all the analyzed genes. It is essential to consider that most studies on invertebrates that involve transcriptional activity analysis use arthropods and short exposure times 14 , 44 – 46 . Limited data are available on mollusks and, usually, they are marine representatives 40 , 47 .…”

Section: Discussionmentioning

confidence: 99%

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

Prieto-Amador

Caballero

Martínez-Guitarte

2021

Sci Rep

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Plastic pollution is one of the leading environmental problems. Phthalates are widely used plastic additives released into the environment. Although the effects of phthalates on vertebrates have been extensively studied, there is a knowledge gap regarding their effects on invertebrates. This work analyzes the impact of three phthalates, diethyl phthalate (DEP), benzyl butyl phthalate (BBP), and bis-(2-ethylhexyl) phthalate (DEHP), on the gastropod Physella acuta at the molecular level to establish the putative pathways involved in its response to them. By real-time PCR, we obtained the expression profile of 30 genes in animals exposed for 1 week to 0.1, 10, and 1000 μg/L of each phthalate. The genes cover DNA repair, detoxification, apoptosis, oxidative and stress responses, immunity, energy reserves, and lipid transport. The results show that while DEP and DEHP did not alter the mRNA levels, BBP modulated almost all the analyzed genes. It can be concluded that the impact of BBP is extensive at the molecular level. However, it cannot be dismissed that the increase in transcriptional activity is a general response due to this compound’s well-known role as an endocrine disruptor. Additional research is needed to elucidate the differences observed in the impact of these compounds on the gastropod P. acuta.

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Prodiamesa olivacea: de novo biomarker genes in a potential sentinel organism for ecotoxicity studies in natural scenarios

Cited by 5 publications

References 65 publications

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Proteogenomic reconstruction of organ-specific metabolic networks in an environmental sentinel species, the amphipodGammarus fossarum

Characterization of Insecticide Response-Associated Transcripts in the Colorado Potato Beetle: Relevance of Selected Cytochrome P450s and Clothianidin

Analysis of the impact of three phthalates on the freshwater gastropod Physella acuta at the transcriptional level

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