Procion Brown MX-5BR attached and Lewis metals ion-immobilized poly(hydroxyethyl methacrylate)/chitosan IPNs membranes: Their lysozyme adsorption equilibria and kinetics characterization

This work presents data on human immunoglobulin G (HIgG) adsorption onto L‐arginine ligand attached poly(GMA/MMA/EGDMA)‐based affinity beads which were synthesized from methyl methacrylate (MMA) and glycidiyl methacrylate (GMA) in the presence of a crosslinker (i.e., ethylene glycol dimethacrylate; EGDMA) by suspension polymerization. The epoxy groups of the poly(GMA/MMA/EGDMA) beads were converted into amino groups after reaction with ammonia or 1,6‐diaminohexane (i.e., spacer‐arm). With L‐arginine as a ligand, it was covalently immobilized on the aminated (poly(GMA/MMA/EGDMA)‐ AA) and/or the spacer‐arm attached (poly(GMA/MMA/EGDMA)‐SA) beads, using glutaric dialdehyde as a coupling agent. Both affinity poly(GMA/MMA/EGDMA)‐based beads were used in HIgG adsorption/desorption studies under defined pH, ionic strength, or temperature conditions in a batch reactor, using acid‐treated poly(GMA/MMA/EGDMA) beads as a control system. The poly(GMA/MMA/EGDMA)‐SA affinity beads resulted in an increase in the adsorption capacity to HIgG compared with the aminated counterpart (i.e., poly(GMA/MMA/EGDMA)‐AA). The maximum adsorption capacities of the poly(GMA/MMA/EGDMA)‐AA and poly(GMA/MMA/EGDMA)‐SA affinity beads were found to be 112.36 and 142 mg g−1, and the affinity constants (Kd), evaluated by the Langmuir model, were 2.48 × 10−7 and 6.98 × 10−7M, respectively. Adsorption capacities of the poly(GMA/MMA/EGDMA)‐AA and poly(GMA/MMA/EGDMA)‐SA were decreased with HIgG by increasing the ionic strength adjusted with NaCl. Adsorption kinetic of HIgG onto both affinity adsorbents was analyzed with first‐ and second‐order kinetic equations. The first‐order equation fitted well with the experimental data. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 672–679, 2007

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“…(3)], with the assumption that a single-site, homogeneous interaction occurs between the protein and the ligand. 19,[21][22][23][24] …”

Section: Adsorption Kinetic and Isotherms Modelsmentioning

confidence: 99%

Adsorption of IgG on spacer‐arm and L‐arginine ligand attached poly(GMA/MMA/EGDMA) beads

Bayramoğlu

Şenel

Arıca

2007

J of Applied Polymer Sci

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“…Of these papers, some showed that the proposed PSO model suitably fitted their experimental data [6][7][8][9][10][11][12][13][14][15]. Some further show that PSO model is more suitable than pseudo-first-order model [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], IPD model [18,21,23,24,27,[33][34][35][36][37][38][39][40][41][42][43], and the Elovich equation [36,42] for this purpose. Only a small number of articles indicated that PSO model is less suitable than other adsorption kinetic models (pseudo-first-order model, IPD model, Elovich equation, etc.).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of pseudo-second-order kinetic model for liquid-phase adsorption: A mini-review

Tseng

Huang

et al. 2009

Chemical Engineering Journal

340

143

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“…Thus, the adsorption occurs spontaneously and it was confirmed by the negative values of DG and the positive values of DS. In addition, the positive values of DH indicate that the adsorption was endothermic [45,46].…”

Section: Thermodynamic Parametersmentioning

confidence: 99%

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

Bayramoğlu

Arıca

2011

Bioprocess Biosyst Eng

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In this work, a new methodology is developed for selection of affinity ligands towards the enzyme "trypsin" using quartz crystals microbalance (QCM) technique. To achieve this goal, the surface amination of gold plated QCM crystals was achieved in 13.56 MHz plasma polymerization system by using ethylenediamine. Three different ligands (i.e., 4-aminobenzamidine, 4-aminobenzoic acid, and phenylalanine) were immobilized on the aminated QCM crystals surface via glutaraldehyde coupling. All three ligand immobilized QCM crystals were characterized and compared under different experimental conditions. It was observed that the benzamidine ligand showed higher affinity to trypsin with a dissociation constant on the order of 1.76 × 10(-9) M, which is within the range of 10(-4)-10(-8) M for affinity ligands. Thus, its selectivity was suitable for purification of trypsin from biological fluids.

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Procion Brown MX-5BR attached and Lewis metals ion-immobilized poly(hydroxyethyl methacrylate)/chitosan IPNs membranes: Their lysozyme adsorption equilibria and kinetics characterization

Cited by 70 publications

References 32 publications

Adsorption of IgG on spacer‐arm and L‐arginine ligand attached poly(GMA/MMA/EGDMA) beads

Adsorption of IgG on spacer‐arm and L‐arginine ligand attached poly(GMA/MMA/EGDMA) beads

Characteristics of pseudo-second-order kinetic model for liquid-phase adsorption: A mini-review

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

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