Processing of rotavirus glycoprotein VP7: implications for the retention of the protein in the endoplasmic reticulum.

Stirzaker, Clare; Whitfeld, Paul R.; Christie, David L.; Bellamy, A R; Both, Gerald W.

doi:10.1083/jcb.105.6.2897

Cited by 66 publications

(48 citation statements)

References 28 publications

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“…This 'leaky scanning' mechanism thus enables two independently-initiated proteins to be produced from one mRNA. Nearly 30 examples of bifunctional mRNAs that fit this description have been identified [18] and, in ten cases, the postulated connection between leaky scanning and a suboptimal context has been confirmed by mutational analysis [19][20][21][22][23][24][25][26][27][28]. Thus, the importance of context for recognition of the AUG initiator codon has been confirmed in many laboratories.…”

Section: Determinants Of Translational Fidelitymentioning

confidence: 78%

Determinants of translational fidelity and efficiency in vertebrate mRNAs

Kozak

1994

Biochimie

124

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Section: Determinants Of Translational Fidelitymentioning

confidence: 78%

Determinants of translational fidelity and efficiency in vertebrate mRNAs

Kozak

1994

Biochimie

124

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“…Simian rotavirus VP7 was excised from pUT‐VP7 with Apa LI/ Xba I and inserted into pNZ‐Usp to obtain pNZ‐Usp‐VP7. This plasmid does not include the two regions of hydrophobic amino acids (H1 and H2), which can act as the signal sequence to direct VP7 to endoplasmatic reticulum (Stirzaker et al. 1987).…”

Section: Methodsmentioning

confidence: 99%

Rotavirus vp7 antigen produced by Lactococcus lactis induces neutralizing antibodies in mice

et al. 2005

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C . A . P E R E Z , C . E I C H W A L D , O . B U R R O N E A N D D . D E M E N D O Z A . 2005.Aims: To determine if live recombinant Lactococcus lactis strains expressing rotavirus VP7 antigen are immunogenic in mice. Methods and Results: Using the food-grade lactic acid bacterium L. lactis as a carrier, we expressed VP7, the major rotavirus outer shell protein and one of the main components of the infective particle, as a cytoplasmic, secreted or cell wall anchored forms. Our results showed that recombinant L. lactis strains secreting VP7 proved to be more immunogenic than strains containing the antigen in the cytoplasm or anchored to the cell wall. Conclusions: This is the first demonstration that recombinant L. lactis producing VP7 can induce the production of a neutralizing antibody response against rotavirus by the intragastric route. Significance and Impact of the Study: Rotaviruses are the single most important aetiological agents of severe diarrhoea of infants and young children worldwide and have been estimated to be responsible for 650 000-800 000 deaths per year of children younger than 5 years old in development countries. Thus, the development of a safe and effective vaccine has been a global public health goal. Although two of five mice orally inoculated with L. lactis strains secreting VP7 elicited a specific-antibody response, these strains could be very useful to be used as a prototype to develop a new generation of protective rotavirus vaccines.

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“…2) [Stirzaker et al, 1987] . The similarities may be partially explained by the classification of G3, G4, and G9 strains into Wa-like genogroup , whereas the G2 study strain and strain RVA/Vaccine/ USA/RotaTeq-SC2-9/1992/G2P7 [5] (Fig.…”

Section: R2 or Ds-1-like Genotypementioning

confidence: 98%

Whole genome analyses of African G2, G8, G9, and G12 rotavirus strains using sequence‐independent amplification and 454® pyrosequencing

Jere

Mlera

O’Neill

et al. 2011

Journal of Medical Virology

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High mortality rates caused by rotaviruses are associated with several strains such as G2, G8, G9, and G12 rotaviruses. Rotaviruses with G9 and G12 genotypes emerged worldwide in the past two decades. G2 and G8 rotaviruses are however also characterized frequently across Africa. To understand the genetic constellation of African G2, G8, G9, and G12 rotavirus strains and their possible origin, sequence-independent cDNA synthesis, amplification, and 454(®) pyrosequencing of the whole genomes of five human African rotavirus strains were performed. RotaC and phylogenetic analysis were used to assign and confirm the genotypes of the strains. Strains RVA/Human-wt/MWI/1473/2001/G8P[4], RVA/Human-wt/ZAF/3203WC/2009/G2P[4], RVA/Human-wt/ZAF/3133WC/2009/G12P[4], RVA/Human-wt/ZAF/3176WC/2009/G12P[6], and RVA/Human-wt/ZAF/GR10924/1999/G9P[6] were assigned G8-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2, G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2, G12-P[4]-I1-R1-C1-M1-A1-N1-T1-E1-H1, G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1, and G9-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genotypes, respectively. The detection of both Wa- and DS-1-like genotypes in strain RVA/Human-wt/ZAF/3133WC/2009/G12P[4] and Wa-like, DS-1-like and P[6] genotypes in strain RVA/Human-wt/ZAF/GR10924/1999/G9P[6] implies that these two strains were generated through intergenogroup genome reassortment. The close similarity of the genome segments of strain RVA/Human-wt/MWI/1473/2001/G8P[4] to artiodactyl-like, human-bovine reassortant strains and human rotavirus strains suggests that it originated from or shares a common origin with bovine strains. It is therefore possible that this strain might have emerged through interspecies genome reassortment between human and artiodactyl rotaviruses. This study illustrates the swift characterization of all the 11 rotavirus genome segments by using a single set of universal primers for cDNA synthesis followed by 454(®) pyrosequencing and RotaC analysis.

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Processing of rotavirus glycoprotein VP7: implications for the retention of the protein in the endoplasmic reticulum.

Abstract: Abstract. Rotaviruses are icosahedral particles that as-

Cited by 66 publications

References 28 publications

Determinants of translational fidelity and efficiency in vertebrate mRNAs

Determinants of translational fidelity and efficiency in vertebrate mRNAs

Rotavirus vp7 antigen produced by Lactococcus lactis induces neutralizing antibodies in mice

Whole genome analyses of African G2, G8, G9, and G12 rotavirus strains using sequence‐independent amplification and 454® pyrosequencing

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