In this study, the single mineral flotation experiments of magnesite and its associated minerals including dolomite, serpentine, talc and quartz were conducted, and the influences of the dosage of collectors such as sodium oleate and lauryl amine, pH value and the influences of regulars such as sodium hexametaphosphate and sodium silicate on those minerals were researched. According to the results, a process to reduce the content of gangue minerals was proposed, which at first use lauryl amine as a collector to remove most talc and quartz and part of the dolomite and serpentine, and then use sodium oleate as a collector to separate magnesite from dolomite and serpentine. This is a theoretical reference for the beneficiation of magnesite.