Processing of precursor RNAs from mitochondria of<i>Neurospora crassa</i>

Agsteribbe, E; Hartog, Marijke

doi:10.1093/nar/15.18.7249

Cited by 15 publications

(6 citation statements)

References 17 publications

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“…However, only the larger 2?3 kb transcript was notably overexpressed at 37˚C. The existence of both pre-processed and processed forms of mitochondrial mRNAs, including the COX1 transcript, has been described previously in several fungi (Agsteribbe & Hartog, 1987;Burger et al, 1985;Costanzo & Fox, 1990;Dyson et al, 1989;Grivell, 1989;Osinga et al, 1984;Tracy & Stern, 1995), plants (Barkan, 1988;Gray & Lovello, 1993;Tracy & Stern, 1995;Wolff & Kuck, 1996) and animals (Ojala et al, 1980;Tracy & Stern, 1995). These pre-processed or precursor RNAs comprise polycistronic transcripts that have not yet been processed into smaller, intermediate and mature forms of RNA.…”

Section: Discussionmentioning

confidence: 68%

“…This common occurrence of transcriptional units in mitochondrial DNA from a variety of plants (Barkan, 1988;Tracy & Stern, 1995;Wolff & Kuck, 1996) and fungi (Agsteribbe & Hartog, 1987;Burger et al, 1985;Costanzo & Fox, 1990;Dyson et al, 1989;Grivell, 1989;Osinga et al, 1984;Tracy & Stern, 1995) implicates their role in facilitating the co-ordinate regulation of gene expression. In our study, the overexpression of a single transcriptional unit encoding ATP9, COX1 and ATP8 mRNAs in C. neoformans suggests that at elevated host temperatures the serotype A strain (H99) upregulates expression of this transcriptional unit.…”

Section: Discussionmentioning

confidence: 94%

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Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment

et al. 2003

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In the study of differential gene expression of Cryptococcus neoformans, a transcript of COX1 (cytochrome oxidase c subunit 1) was identified in a serotype A strain. The transcript was upregulated at 37˚C compared to 30˚C and expressed by yeasts infecting the central nervous system. Northern analysis of COX1 from the serotype A strain revealed two polycistronic transcripts, a temperature-upregulated 2?3 kb transcript and a 1?9 kb transcript that was not affected by temperature. In contrast, COX1 in a serotype D strain showed only a 1?9 kb polycistronic transcript plus a 1?6 kb monocistronic message, and temperature had no effect on the transcripts. The sequence of COX1 revealed similar coding regions between the two strains, but the serotype D strain had five introns whereas no introns were found in the serotype A strain. The serotype D strain had reduced growth rates compared to the serotype A strain at 37˚C, but in an AD hybrid strain the serotype D COX1 gene could support efficient high temperature growth. These studies have revealed mitochondrial molecular differences between serotype A and D strains which show evolutionary divergence. It will be important to determine whether differences in mitochondrial structure and function can influence cryptococcosis. INTRODUCTIONCryptococcus neoformans is an encapsulated yeast with a propensity for invading the central nervous system of healthy and immunocompromised individuals. Since the beginning of the AIDS pandemic (in the early 1980s), the occurrence of cryptococcal infection has increased sharply, and although several antifungal drugs, including amphotericin B, flucytosine, fluconazole and itraconazole, have shown moderate efficacy in treatment of this infection, a significant number of relapses and treatment failures still occur (Perfect, 1989). The discovery of new antifungal agents which selectively kill or inhibit the growth of C. neoformans in the host is needed for more effective management of this serious and sometimes fatal infection.Recent studies into the pathobiology of C. neoformans have established effective molecular tools to examine the pathogenicity of this fungus and thus identify potential genetic targets for drugs (Chang et al., 1996; Chang & KwonChung, 1994;Lodge et al., 1994;Odom et al., 1997; Salas et al., 1996). Several C. neoformans genes disrupted by targeted gene replacement have now been shown to be associated with the pathogenicity of this yeast. Some examples are those for capsule formation (Chang et al., 1996), laccase for melanin production (Salas et al., 1996), urease , phospholipase and mannose metabolism (Wills et al., 2001). Signal transduction pathways for control of virulence include calcineurin (Odom et al., 1997), GPA1 (Alspaugh et al., 1997), PKA1 (D' Souza et al., 2001) and RAS1 (Alspaugh et al., 2000), which regulate genes for the three major virulence phenotypes of capsule, melanin and growth at 37˚C.The specific hypothesis for this study is that detectable changes in gene expression directed by certain pathobiologic...

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 94%

Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment

et al. 2003

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“…The three genes CO-III, ATPase-6 and ND-3 are linked closely in the mitochondrial genome, 17 and posttranscriptional processing is necessary to produce mature protein-encoding genes. 23 The precursor mitochondrial transcript containing CO-III, ATPase-6, and ND-3 has been demonstrated in human tissue. 24 In our study, CO III, ATPase-6, and ND-3 were shown to be contained in a precursor mRNA species and downregulated by 400-lux light rearing.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of ATP Synthase Subunit-6, CytochromecOxidase-III, and NADH Dehydrogenase-3 by Bright Cyclic Light in the Rat Retina

Alvarez

et al. 2004

Invest. Ophthalmol. Vis. Sci.

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Rearing in cyclic light at 400-lux downregulates the expression of ATPase-6, CO-III, and ND-3 compared with rearing in 5-lux cyclic light. The authors hypothesize that these changes are adaptive responses to light stress that provide neuroprotection to retinal cells by elevating the level of stress-related factors and reducing the level of oxidized cytochrome c, the form that activates the apoptotic cascade of cell death.

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“…In Neurospora, Aspergillus and Schizosaccharomyces mitochondria, polycistronic transcripts were found to be processed by tRNA excision and additional processing events, generating mono-or dicistronic RNA species (e.g. [26,16,14,1,45,8]). In Kluyveromyces and Saccharomyces, multiple promoters exist for several small transcriptional units [63,22], and processing occurs by endonucleolytic cleavage close to tRNAs or at conserved sequence motifs [60,12].…”

Section: Polycistronic Transcripts Undergo Substantial Processing Eventsmentioning

confidence: 99%

“…For example, compact animal mtDNAs are transcribed into precursor molecules, which subsequently are processed to monocistronic RNAs by excision of individual tRNAs [58,59]. A similar mode of RNA processing seems to be employed in mitochondria of Aspergillus and Neurospora, where tRNA are used as processing signals [16,14,1,26 ]. However, mitochondrial DNA from the yeast Saccharomyces cerevisiae carries multiple transcription units, which are controlled by their own promoters [22].…”

Section: Introductionmentioning

confidence: 99%

Transcript mapping and processing of mitochondrial RNA in the chlorophyte alga Prototheca wickerhamii

Wolff

Kück

1996

Plant Mol Biol

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The detailed transcript map of the circular 55328 bp mitochondrial (mt) genome from the colourless chlorophycean alga Prototheca wickerhamii has been determined. On each half of this genome the genes are encoded only on one DNA strand, forming transcriptional units comprising variable numbers of genes. With the exception of four genes coding for ribosomal proteins, transcripts of the three rRNA genes and all protein-coding genes have been detected by both northern analysis and primer extension experiments. Polycistronic transcripts of protein coding and tRNA genes were verified by northern analyses, primer extension and RNAse mapping experiments. The 5' and 3' ends of different RNA species are often located in close proximity to putative stem-loop structures and some 5' termini of mRNAs coincide with the 3' end of tRNAs located immediately upstream. Transcript mapping in a putative promoter region revealed two different possible transcription initiation sites; no significant sequence homology to putative mt promoters from higher plants could be found. In addition, two out of three group I introns residing in the cox1 gene were found to be self-splicing in vitro under reaction conditions developed for related mt introns from a filamentous fungus. Mitochondrial gene expression of P. wickerhamii and of filamentous fungi has several features in common, such as intron splicing and the processing of longer polycistronic transcripts. The similarities in RNA maturation between higher-plant and P. wickerhamii mitochondria are less pronounced, since plants rarely use tRNAs as processing signals for their relatively short mitochondrial co-transcripts.

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Processing of precursor RNAs from mitochondria ofNeurospora crassa

Cited by 15 publications

References 17 publications

Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment

Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment

Downregulation of ATP Synthase Subunit-6, CytochromecOxidase-III, and NADH Dehydrogenase-3 by Bright Cyclic Light in the Rat Retina

Transcript mapping and processing of mitochondrial RNA in the chlorophyte alga Prototheca wickerhamii

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