2002
DOI: 10.1128/jvi.76.4.2003-2008.2002
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Processing of Nonstructural Protein 1a of Human Astrovirus

Abstract: Astrovirus contains three open reading frames (ORF) on its genomic RNA, ORF1a, ORF1b, and ORF2. ORF1a encodes a 920-amino-acid (aa) nonstructural protein, nsP1a, which displays a 3C-like serine protease motif. Little is known about the processing of nsP1a or whether the protease it contains is active and involved in autocatalytic processing. Here we address both of these matters. Intact and N-terminally deleted forms of ORF1a from human astrovirus serotype 1 were expressed in BHK cells, and nsP1a-derived proce… Show more

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Cited by 52 publications
(53 citation statements)
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“…The full-length polyproteins nsp1a and nsp1ab, with calculated molecular masses of 103 and 160 kDa, respectively, were not found in cell lysates from Yuc8-infected or plasmid-transfected cells, suggesting that cotranslational processing occurs during their synthesis, as previously shown for HAstV-1 in transient expression experiments (5). Two proteins of around 19 to 20 kDa seem to represent the N-terminal products of the cotranslational processing of polyproteins nsp1a and nsp1b during astrovirus infection.…”
Section: Discussionmentioning
confidence: 52%
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“…The full-length polyproteins nsp1a and nsp1ab, with calculated molecular masses of 103 and 160 kDa, respectively, were not found in cell lysates from Yuc8-infected or plasmid-transfected cells, suggesting that cotranslational processing occurs during their synthesis, as previously shown for HAstV-1 in transient expression experiments (5). Two proteins of around 19 to 20 kDa seem to represent the N-terminal products of the cotranslational processing of polyproteins nsp1a and nsp1b during astrovirus infection.…”
Section: Discussionmentioning
confidence: 52%
“…Few studies have examined the synthesis and processing of the HAstV nonstructural proteins, and many details of these events remain to be elucidated (5,7,11,24). In this study we contribute to the characterization of the processing of the nonstructural polyproteins of astroviruses, using as a model an HAstV-8 strain.…”
Section: Resultsmentioning
confidence: 99%
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