Processing of major ABO‐incompatible bone marrow for transplantation by using dextran sedimentation

Abstract

DS is an efficient method of depleting RBCs in major ABO-incompatible BM allografts without significant loss of HPCs.

Red blood cell-incompatible allogeneic hematopoietic progenitor cell transplantation

“…Apheresis devices and hydroxyethylstarch or dextran sedimentation are commonly used to remove red cells from marrow components for major ABO incompatibility. 62,69,70,71 Braine et al 69 were able to reduce the red cell contents of BM grafts from a mean of 354±115 to 21.0±9.5 mL by processing with a Haemonetics (Braintree, MA, USA) apheresis device with manual collection of buffy-coat cells. Nine of 15 patients transplanted experienced symptoms of a hemolytic transfusion reaction including fever, chills and hypertension, although the reactions may have been ameliorated by preinfusion medication with diphenhydramine, hydrocortisone and mannitol.…”

ABO‐mismatched marrow processing for transplantation: results of 114 procedures and analysis of immediate adverse events and hematopoietic recovery

“…In case of ABO mismatch between donor and recipient, however, bone marrow (BM) manipulation for red cell (RBC) depletion is an important consideration to minimize the risk of hemolytic reactions. Different semiautomated or automated techniques have thus been developed to remove RBCs from BM with the aim of maximizing recovery of nucleated cells and CD34+ hematopoietic stem cells 3‐7 . The use of flow cell separator is now a well‐established method to ensure both high BM RBC depletion and high recovery rate of hematopoietic progenitor cells.…”

“…Different semiautomated or automated techniques have thus been developed to remove RBCs from BM with the aim of maximizing recovery of nucleated cells and CD34+ hematopoietic stem cells. [3][4][5][6][7] The use of flow cell separator is now a well-established method to ensure both high BM RBC depletion and high recovery rate of hematopoietic progenitor cells. The side effects occurring at the time of graft infusion and the potential involvement of residual RBCs in these adverse events, however, have not been described in large studies.…”

Outcomes of processing of bone marrow harvests for hematopoietic stem cell transplantation in pediatric patients utilizing a novel red blood cell sedimentation kit

“…Although the original product development and testing of PrepaCyte was for minimal manipulation of BM as the primary application, the manufacturer subsequently adapted the system for use with CB. The system has been used successfully at the St Louis Cord Blood Bank for manufacturing of ALLOCORD, a FDA licensed HPC CB product . PrepaCyte‐CB was found to be effective in recovering the targeted WBC and TNC populations while reducing the amount of RBCs during CB processing…”

“…Alternative methods for BM processing have become available in recent past. Some involve use of devices based on centrifugation (Optia continuous flow), and others involve chemical reagents (PrepaCyte) . In this report we will describe our experience with BM processing with PrepaCyte‐CB kit (BioE).…”