Processing of fluorescence angiograms for the quantification of vascular effects induced by anti-angiogenic agents in the CAM model

Nowak-Śliwińska, Patrycja; Ballini, Jean‐Pierre; Wagnières, Georges; Bergh, Hubert van den

doi:10.1016/j.mvr.2009.10.004

Cited by 62 publications

(61 citation statements)

References 22 publications

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“…As the revascularization of the CAM occurs from the edge of the PDT-treated area, we have now adopted the previously described software [25] to create four concentric circular areas. In each area the vascularization is monitored and quantified as a function of time after PDT, normalized per area.…”

Section: Discussionmentioning

confidence: 99%

“…In this assay, the anti-angiogenic efficacy of the compounds was tested in the physiologically developing CAM model between EDD7 and EDD9, as previously described in detail [25]. Briefly, compounds were applied topically twice (each time 20 l) on EDD7 and EDD8.…”

Section: Developmental Cam and Quantification Of The Angiogenic Responsementioning

confidence: 99%

“…The in ovo CAM model for Visudyne ® -PDT Fertilized chicken eggs were labelled and transferred into a hatching incubator with a relative air humidity of 65% and a temperature of 37ЊC, as described previously [23,25]. On EDD3, a hole of approximately 3 mm in diameter was opened in the eggshell and covered with a laboratory wrapping film, Parafilm ® (Pechiney, Menasha, WI, USA) to prevent dehydration and possible infections.…”

Section: Microscopy and Image Acquisitionmentioning

confidence: 99%

“…Study of photodynamic angio-occlusion and reperfusion, as well as neoformation of vessels can be performed in situ and in real time [23][24][25][26]. This in vivo pre-clinical model has the advantage of having a thin, planar vascular network, which is well-accessible to PDT and PDT in combination with adjuvants, added either topically or intravenously (i.v.)…”

Section: Introductionmentioning

confidence: 99%

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Angiostatic kinase inhibitors to sustain photodynamic angio-occlusion

Nowak-Śliwińska

Weiss

Beijnum

et al. 2012

Vascular Pharmacology

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Targeted angiostatic therapy receives major attention for the treatment of cancer and exudative age-related macular degeneration (AMD). Photodynamic therapy (PDT) has been used as an effective clinical approach for these diseases. As PDT can cause an angiogenic response in the treated tissue, combination of PDT with anti-angiogenic compounds should lead to improved therapy. This study was undertaken to test the clinically used small molecule kinase inhibitors Nexavar ® (sorafenib), Tarceva ® (erlotinib) and Sutent ® (sunitinib)for this purpose, and to compare the results to the combination of Visudyne ® -PDT with Avastin ® (bevacizumab) treatment. When topically applied to the chicken chorioallantoic membrane at embryo development day (EDD) 7, a clear inhibition of blood vessel development was observed, with sorafenib being most efficient. To investigate the combination with phototherapy, Visudyne ® -PDT was first applied on EDD11 to close all Ͻ100 m vessels. Application of angiostatics after PDT resulted in a significant decrease in vessel regrowth in terms of reduced vessel density and number of branching points/mm 2 . As the 50% effective dose (ED50) for all compounds was approximately 10-fold lower, Sorafenib outperformed the other compounds. In vitro, all kinase inhibitors decreased the viability of human umbilical vein endothelial cells. Sunitinib convincingly inhibited the in vitro migration of endothelial cells. These results suggest the therapeutic potential of these compounds for application in combination with PDT in anti-cancer approaches, and possibly also in the treatment of other diseases where angiogenesis plays an important role.

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Section: Discussionmentioning

confidence: 99%

Section: Developmental Cam and Quantification Of The Angiogenic Responsementioning

confidence: 99%

Section: Microscopy and Image Acquisitionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Angiostatic kinase inhibitors to sustain photodynamic angio-occlusion

Nowak-Śliwińska

Weiss

Beijnum

et al. 2012

Vascular Pharmacology

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“…Morphological changes in the CAM organization recorded on development day 15 were imaged via epifluorescence microscopy and quantified using an automated imageprocessing method with a descriptor of branching points/ mm 2 (Figure 4). 43 Complex 1 administered at 50 μM (0.5 mg/kg) was previously shown to induce an antivascular effect represented by a 26.3% reduction in the number of branching points/ mm 2 . 28 The presence of avascular zones (black areas in fluorescence angiographies indicated by arrows, Figure 4) was observed for 2 administered at 42 μM (0.47 mg/kg), with a 38% reduction of the number branching points/mm 2 versus the control (***p = 2.8 × 10 −5 ), mainly along the larger vessels.…”

Section: Molecular Pharmaceuticsmentioning

confidence: 99%

Antiangiogenic and Anticancer Properties of Bifunctional Ruthenium(II)–p-Cymene Complexes: Influence of Pendant Perfluorous Chains

et al. 2015

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Two bifunctional ruthenium(II)-p-cymene complexes with perfluorinated side chains, attached via pyridine ligands, have been evaluated in a series of in vitro and in vivo assays. Their effects on human endothelial (ECRF24 and HUVEC) cells, noncancerous human embryonic kidney (HEK-293) cells, and various human tumor cells were investigated. The complex with the shorter chain, 1, inhibits the proliferation of the tumor cell lines and ECRF24, whereas 2 selectively inhibits ECRF24 and HUVEC proliferation. Neither inhibits the migration of ECRF24 cells whereas both compounds inhibit sprout formation in HUVEC cells. Using three preclinical models, i.e., vasculature formation in the chorioallantoic membrane (CAM) of the chicken embryo, human A2780 ovarian carcinoma tumors xenografted on the CAM, and human LS174T colorectal adenocarcinoma tumors grown in athymic mice, the angiostatic and anticancer activities of these two complexes were studied. Overall, 1 inhibited tumor growth predominantly through an anticancer effect whereas 2 inhibited tumor growth predominately via an antiangiogenic mechanism.

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Human Chorionic Membrane‐derived Tunable Hydrogels for Vascular Tissue Engineering Strategies

Martins,

Deus,

Gomes

et al. 2024

Adv Healthcare Materials

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One of the foremost targets in the advancement of biomaterials to engineer vascularized tissues is not only to replicate the composition of the intended tissue but also to create thicker structures incorporating a vascular network for adequate nutrients and oxygen supply. For the first time, to the best of current knowledge, a clinically relevant biomaterial is developed, demonstrating that hydrogels made from the human decellularized extracellular matrix can exhibit robust mechanical properties (in the kPa range) and angiogenic capabilities simultaneously. These properties enable the culture and organization of human umbilical vein endothelial cells into tubular structures, maintaining their integrity for 14 days in vitro without the need for additional polymers or angiogenesis‐related factors. This is achieved by repurposing the placenta chorionic membrane (CM), a medical waste with an exceptional biochemical composition, into a valuable resource for bioengineering purposes. After decellularization, the CM underwent chemical modification with methacryloyl groups, giving rise to methacrylated CM (CMMA). CMMA preserved key proteins, as well as glycosaminoglycans. The resulting hydrogels rapidly photopolymerize and have enhanced strength and customizable mechanical properties. Furthermore, they demonstrate angio‐vasculogenic competence in vitro and in vivo, holding significant promise as a humanized platform for the engineering of vascularized tissues.

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Processing of fluorescence angiograms for the quantification of vascular effects induced by anti-angiogenic agents in the CAM model

Cited by 62 publications

References 22 publications

Angiostatic kinase inhibitors to sustain photodynamic angio-occlusion

Angiostatic kinase inhibitors to sustain photodynamic angio-occlusion

Antiangiogenic and Anticancer Properties of Bifunctional Ruthenium(II)–p-Cymene Complexes: Influence of Pendant Perfluorous Chains

Human Chorionic Membrane‐derived Tunable Hydrogels for Vascular Tissue Engineering Strategies

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