Processing of autologous bone marrow cells by apheresis technology for cell-based cardiovascular regeneration

Dettke, Markus; Leitner, Gerda; Kopp, Christoph W.; Chen, Ying; Gyöngyösi, Mariann; Lang, Irene M.

doi:10.3109/14653249.2012.690509

Cited by 3 publications

(3 citation statements)

References 43 publications

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“…This process can take hours to complete. A labeless method of separating RBCs from stem cells that does not require the addition of sedimentation agents, that would not harm the stem cells, and that would be more rapid than the current method would be highly desirable (Basford, Forraz, Habibollah, Hanger, & McGuckin, 2010;Dettke et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study

Moore

Mizutani

Tanaka

et al. 2018

Biotech & Bioengineering

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The ability to separate RBCs from the other components of whole blood has a number of useful clinical and research applications ranging from removing RBCs from typical clinical blood draw, bone marrow transplants to transfusions of these RBCs to patients after significant blood loss. Viewed from a mechanistic/process perspective, there are three routine methodologies to remove RBCs: 1) RBCs lysis, 2) separation of the RBCs from the nucleated cells (i.e., stem cells) based on density differences typically facilitated through centrifugation or sedimentation agents, and 3) antibody based separation in which a targeted RBC is bound with an affinity ligand that facilitates its removal. More recently, several microfluidic based techniques have also been reported. In this report, we describe the performance of continuous RBC separation achieved by the deflection of intrinsically magnetic, deoxygenated RBCs as they flow through a magnetic energy gradient created by quadrupole magnet. This quadrupole magnetic, with aperture of 9.65 mm, has a maximum field of B = 1.36 T at the pole tips and a constant field gradient of B /r = 286 T/m. The annular flow channel, contained within this quadrupole magnet, is 203 mm long, has an inner radius of 3.98 mm, and an inner, outer radius of 4.36 mm, which corresponds to an annulus radius of 380 micrometer. At the entrance and exit to this annular channel, a manifold was designed which allows a cell suspension and sheath fluid to be injected, and a RBC enriched exit flow (containing the magnetically deflected RBCs) and a RBC depleted exit flow to be collected. Guided by theoretical models previously published, a limited number of operating parameters; total flow rate, flow rate ratios of flows in and flow out, and ratios of RBC to polystyrene control beads was tested. The overall performance of this system is consistent with our previously presented, theoretical models and our intuition. As expected, the normalized recovery of RBCs in the RBC exit fraction ranged from approximately 95% down to 60%, as the total flow rate through the system increased from 0.1 to 0.6 ml/min. At the cell concentrations studied, this corresponds to a flow rate of 1.5 × 10 -9 × 10 cells/min. While the throughput of these pilot scale studies are slow for practical applications, the general agreement with theory, and the small cross-sectional area in which the actual separation is achieved, 77 mm (annulus radius times the length), and corresponding volume of approximately 2 mls, suggests the potential to scale-up a system for practical applications exists and is actively being pursued.

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Section: Introductionmentioning

confidence: 99%

Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study

Moore

Mizutani

Tanaka

et al. 2018

Biotech & Bioengineering

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“…One of them is purifying biological samples from an excess of RBCs for diagnostic or therapeutic applications. For instance, isolation of RBCs form stem cells is desirable at bone marrow transplantation [249]. Additionally, it is known that blood transfusion may be associated with increased side effects due to the damage of RBCs after long storage [250,251].…”

Section: Magnetic Cell Separationmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…Various methods have been used for RBC depletion such as RBC sedimentation using Hydroxyethyl starch (HES) sedimentation or density gradientbased separation (DGBS) over Ficoll-Paque using COBE 2991 (Terumo BCT) [3] or not [4,5]. Studies have demonstrated feasibility and clinically efficiencies for centrifugation using automated or semi-automated separator devices such as Haemonetics Model 30 [6], Amicus (Fresenius-Kabi) and Fenwal CS3000 Omnix plus devices [7] or COBE Spectra [3,8,9], albeit with some quantitative variations between methods for RBC depletion and hematopoietic progenitor cells (HPC) recovery. The newly available BMP kit (Bone Marrow Program) available with the Spectra Optia device achieved results in a similar range as those reported for Optia's predecessor technology, the COBE Spectra [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

A comparison of two protocols for optimal red blood cell depletion using Sepax-2 device for ABO-major incompatible transplantation in adults

Fantin

Olivieri

Spirito-Daffara

et al. 2019

Current Research in Translational Medicine

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Processing of autologous bone marrow cells by apheresis technology for cell-based cardiovascular regeneration

Cited by 3 publications

References 43 publications

Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study

Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

A comparison of two protocols for optimal red blood cell depletion using Sepax-2 device for ABO-major incompatible transplantation in adults

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