Processing Map and Essential Cleavage Sites of the Nonstructural Polyprotein Encoded by ORF1 of the Feline Calicivirus Genome

Sosnovtsev, Stanislav V.; Garfield, Mark; Green, Kim Y.

doi:10.1128/jvi.76.14.7060-7072.2002

Cited by 107 publications

(128 citation statements)

References 38 publications

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“…Recruitment of host cell translation initiation factors to the cytoplasmic replication factories produced during vaccinia virus replication (24) may have contributed to the inefficient NoV replication, structural protein translation, and particle assembly. Use of MVA/T7 to establish reverse genetics systems for calicivirus has varied and is successful with feline calicivirus (FCV) (25) but not porcine enteric calicivirus (PEC) (26) or MNV (27). A modified fowlpox virus expressing T7 RNA polymerase (FPV-T7) reverse genetics system recovers infectious MNV from cells transfected with a full-length MNV cDNA clone, but vaccinia virus inhibits MNV replication (27).…”

Section: Discussionmentioning

confidence: 99%

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Plasmid-based human norovirus reverse genetics system produces reporter-tagged progeny virus containing infectious genomic RNA

Katayama

Murakami

Sharp³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Human noroviruses are the predominant cause of acute gastroenteritis worldwide, but they remain noncultivatable. A tractable system is needed to understand the host restriction to cultivation. We established a reverse genetics system driven by a mammalian elongation factor-1α promoter without helper virus. This system supports genome replication, particle formation, and particles containing a GFP-marked genomic RNA. RNA from these particles is infectious. The system also produces infectious murine norovirus, confirming its broad applicability to other noroviruses.

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Section: Discussionmentioning

confidence: 99%

“…Finally, we evaluated this system using the MNV S7 strain (24)(25)(26), which is cultivable in murine macrophage cells. The pMNV S7F plasmid, which contains the full genome of MNV S7 strain (Fig.…”

Section: Cellular Localization Of the Nov Nonstructural Proteins Exprmentioning

confidence: 99%

Plasmid-based human norovirus reverse genetics system produces reporter-tagged progeny virus containing infectious genomic RNA

Katayama

Murakami

Sharp³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…The infectious virion is composed of 180 copies of the major capsid protein VP1 and 1 to 10 copies of the minor structural protein VP2, which together form a capsid around the VPg-linked genome (10-15). The genomic RNA serves as a template for translation of the ORF1 polyprotein that is proteolytically cleaved by the viral protease to release the nonstructural proteins (16,17), and an ϳ2.2-to 2.6-kb subgenomic bicistronic RNA template is used for the translation of VP1 and VP2 (7, 18). A genetic feature unique to members of the genus Vesivirus is expression of the major capsid protein from ORF2 as a precursor protein (5,(18)(19)(20).…”

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confidence: 99%

“…Virus was recovered from plasmid DNA with the modified vaccinia virus Ankara (MVA)-T7 expression system, as described previously (16). Briefly, confluent CRFK cell monolayers in 6-well plates (approximately 2 ϫ 10 6 cells) were infected with MVA-T7 (a gift from Bernard Moss) at a multiplicity of infection (MOI) of 10 and incubated for 1 h at 37°C.…”

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confidence: 99%

The Feline Calicivirus Leader of the Capsid Protein Is Associated with Cytopathic Effect

Abente

Sosnovtsev

Sandoval-Jaime

et al. 2013

J Virol

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Open reading frame 2 (ORF2) of the feline calicivirus (FCV) genome encodes a capsid precursor that is posttranslationally processed to release the mature capsid protein (VP1) and a small protein of 124 amino acids, designated the leader of the capsid (LC). To investigate the role of the LC protein in the virus life cycle, mutations and deletions were introduced into the LC coding region of an infectious FCV cDNA clone. Three cysteine residues that are conserved among all vesivirus LC sequences were found to be critical for the recovery of FCV with a characteristic cytopathic effect in feline kidney cells. A cell-rounding phenotype associated with the transient expression of wild-type and mutagenized forms of the LC correlated with the cytopathic and growth properties of the corresponding engineered viruses. The host cellular protein annexin A2 was identified as a binding partner of the LC protein, consistent with a role for the LC in mediating host cell interactions that alter the integrity of the cell and enable virus spread.M embers of the family Caliciviridae are small nonenveloped viruses that contain a positive-sense single-stranded RNA genome. Feline calicivirus (FCV) is in the genus Vesivirus of the family and has been an important model for studying calicivirus replication because it grows efficiently in cell culture and has a reverse genetics system (1-5). The RNA genomes of caliciviruses range in size from ϳ6.7 to 8.5 kb and typically encode 8 or 9 viral proteins from two (Sapovirus, Nebovirus, and Lagovirus) or three (Norovirus and Vesivirus) open reading frames (ORFs). A unique ORF4 in the murine norovirus genome that encodes a protein reported to downregulate the innate immune response has been identified (6). The 5= end of the viral RNA genome is covalently linked to a VPg protein; the 3= end of the genome is polyadenylated (2, 7-9). The infectious virion is composed of 180 copies of the major capsid protein VP1 and 1 to 10 copies of the minor structural protein VP2, which together form a capsid around the VPg-linked genome (10-15). The genomic RNA serves as a template for translation of the ORF1 polyprotein that is proteolytically cleaved by the viral protease to release the nonstructural proteins (16,17), and an ϳ2.2-to 2.6-kb subgenomic bicistronic RNA template is used for the translation of VP1 and VP2 (7, 18). A genetic feature unique to members of the genus Vesivirus is expression of the major capsid protein from ORF2 as a precursor protein (5,(18)(19)(20). This precursor is processed in trans by the viral protease to release two proteins: the leader of the capsid (LC) and the mature capsid protein (VP1) (5,19,21,22). The function of the LC protein is not clear, but cleavage of the precursor to release LC and VP1 is essential for the recovery of infectious virions (5). Transient expression of the LC was reported to enhance replication of a human norovirus RNA replicon (23), and an increase in the level of mRNA for the low-density lipoprotein receptor (LDLR) was observed (24). We showed previo...

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“…The ORF1 (nucleotides 20-5305) encodes a large polyprotein, which undergoes cotranslational cleavage yielding 6 nonstructural proteins ranging in size from 5.6 to 75.6 kD. 4,33,36,43 The ORF2 (nucleotide 5314 to 7317-7326) encodes the precursor of the major structural capsid protein, 5,22 which later undergoes cleavage by a viral-encoded proteinase to a 65-66-kD mature protein. 35 The capsid protein is divided into 6 areas on the basis of genetic variability, designated A, B, C, D, E, and F representing amino acids residues 1-120, 121-396, 397-401, 402-425, 426-520, and 521-668, respectively.…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Feline Caliciviruses Associated with a Hemorrhagic-Like Disease

2005

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Abstract. Feline calicivirus (FCV) is 1 of the most common causes of upper respiratory tract disease in cats. Other disease syndromes associated with FCV infection have been reported. Recently, calicivirus infection associated with a hemorrhagic-like disease leading to significant mortality in cats has been reported. The clinical signs are similar to those observed with the calicivirus of rabbit hemorrhagic disease. This study characterized 2 FCV isolates associated with hemorrhagic-like disease. Nucleotide sequencing of the complete genome has been done for these 2 isolates as well as for 4 additional isolates representing other disease syndromes. Previously reported sequence data for the entire genome of classical FCV (6 isolates) and a portion of the capsid gene for hemorrhagic-like FCV (3 isolates), isolated in different regions of United States were used in the genetic analysis. Sequence data were used to determine relationships among the isolates and any correlation with phenotype. Nucleotide sequence comparisons of the entire genome and individual open reading frames revealed high homology among all isolates. Data suggest that the virulence may have genetic determinants on the basis of phylogenetic clustering of the isolates associated with hemorrhagic-like disease.

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Processing Map and Essential Cleavage Sites of the Nonstructural Polyprotein Encoded by ORF1 of the Feline Calicivirus Genome

Cited by 107 publications

References 38 publications

Plasmid-based human norovirus reverse genetics system produces reporter-tagged progeny virus containing infectious genomic RNA

Plasmid-based human norovirus reverse genetics system produces reporter-tagged progeny virus containing infectious genomic RNA

The Feline Calicivirus Leader of the Capsid Protein Is Associated with Cytopathic Effect

Genetic Analysis of Feline Caliciviruses Associated with a Hemorrhagic-Like Disease

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