Processing, assembly, and immunogenicity of human immunodeficiency virus core antigens expressed by recombinant vaccinia virus

Distinct signals in human immunodeficiency virus type 1 Pr55 necessary for RNA binding and particle formation

“…The ability of gag gene products to self-assemble following expression has been established for a number of retroviruses, bovine (Rasmussen et al, 1990), feline (Morikawa et al, 1991), human Karacostas et al, 1989;Overton et al, 1989;Hu et al, 1990;Luo et al, 1990;Shioda & Shibuta, 1990;Smith et al, 1990;Haffar et al, 1991;Hoshikawa et al, 1991;Royer et al, 1991;Mergener et al, 1992) and simian immunodeficiency viruses (Delchambre et al, 1989). However, the boundaries of the gag sequences necessary for particle formation have not been systematically determined.…”

“…In this way, the enzymatic functions necessary for virus infectivity (the protease, reverse transcriptase, RNase H and integrase) are incorporated into the budding particle. When expressed alone in a number of eukaryotic expression systems, Pr55 produces virus-like particles very similar to the early budding immature particles seen in HIV-infected cells Karacostas et al, 1989;Overton et al, 1989;Hu et al, 1990;Luo et al, 1990;Shioda & Shibuta, 1990;Smith et al, 1990;Haffar et al, 1991 ;Hoshikawa et al, 1991 ;Royer et al, 1991 ;Mergener et al, 1992). The ability of similar core antigens from other retroviruses to self-assemble into particle structures is now well documented (Delchambre et al, 1989;Rasmussen et al, 1990;Morikawa et al, 1991).…”

Cytotoxic and proliferative T cell responses in HIV-1-infected Macaca nemestrina.

“…Abbreviations used in this paper: APC, antigen-presenting cells; BLCL, B Recombinant vaccinia viruses (rVV). The entire gag and env genes of HIV-1 LAI were inserted into wild type vaccinia (New York City Board of Health strain) as previously described (11,12) (kindly donated by S.-L. Hu, Bristol Meyers, Squibb, Seattle, WA). The entire nef gene of HIV-1 LAI was inserted into wild type vaccinia, as previously described (13) (vTFnef kindly donated by S. Koenig, MedImmune, Inc., Gaithersburg, MD).…”

Pathogenesis of Human Immunodeficiency Virus Infection

“…Coinfection of cells with vaccinia virus constructs containing the core or envelope region of HIV give rise to virus-like particles that express gpl20 on the surface and that lack the HIV genome (420,494,1093) (Fig. 27).…”