Background: Sequoia sempervirens (D. Don) Endl.) (redwood) has the potential to be grown in New Zealand in commercial forestry operations and is valued for its naturally durable heartwood. A viable redwood industry based on planted forests can only be achieved if the timber produced meets quality expectations, in particular durability. Natural durability is highly variable among trees. Also, a within-tree pattern of low durability close to the pith has been observed. Natural durability is preliminarily caused by secondary metabolites deposited into the cell walls during heartwood formation. The exact nature of the compounds responsible for natural durability in redwood is unknown. Methods: Samples of heartwood from 22 different trees were obtained, ground and extracted using a range of solvents. The ability of some of these extracts to reduce the growth of two fungi (Gloeophyllum trabeum and Trametes versicolor) was tested in vitro. Information on the composition of the extracts was obtained using infrared spectroscopy and gas chromatography.