Processed short neuropeptide F peptides regulate growth through the ERK‐insulin pathway in <i>Drosophila melanogaster</i>

Lee, Kyu-Sun; Hong, Seung-Hyun; Kim, Ae-Kyeong; Ju, Sung-Kyu; Kwon, O‐Yu; Yu, Kweon

doi:10.1016/j.febslet.2009.07.024

Cited by 30 publications

(25 citation statements)

References 23 publications

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“…Furthermore, selective rescue of sNPF expression under the control of GMR20D06- or GMR21B10-GAL4 in the sNPF hypomorphic mutant background restored the starvation-dependent decrease in bitter sensitivity (Figure 4F 2–3 ; compare to the phenotype of sNPF hypomorphic mutants in Figure 3C 2–3 ). By contrast, driving UAS-sNPF expression in a different subset of sNPF + neurons labeled by a line called sNPF-GAL4 (Lee et al, 2009) did not rescue the mutant phenotype (Figure 4F 1 and S4D). Therefore, sNPF expression in a specific subset of sNPF + neurons (LNCs) is necessary and sufficient for the effect of this neuropeptide to regulate bitter sensitivity.…”

Section: Resultsmentioning

confidence: 95%

“…To identify the subset of sNPF-expressing neurons (sNPF + neurons) that controls bitter sensitivity, we genetically silenced different subsets of sNPF + neurons by driving KIR2.1 expression using a panel of 11 GAL4 lines each containing different DNA fragments from the sNPF gene (Lee et al, 2009; Pfeiffer et al, 2008). Expression of KIR2.1 was restricted to adulthood using Gal80 ts , and bitter sensitivity was analyzed after 1 day of wet starvation.…”

Section: Resultsmentioning

confidence: 99%

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Independent, Reciprocal Neuromodulatory Control of Sweet and Bitter Taste Sensitivity during Starvation in Drosophila

Inagaki

Panse

Anderson

2014

Neuron

167

224

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SUMMARY An organism’s behavioral decisions often depend upon the relative strength of appetitive and aversive sensory stimuli, the relative sensitivity to which can be modified by internal states like hunger. However, whether sensitivity to such opposing influences is modulated in a unidirectional or bidirectional manner is not clear. Starved flies exhibit increased sugar and decreased bitter sensitivity. It is widely believed that only sugar sensitivity changes, and that this masks bitter sensitivity. Here we use gene- and circuit-level manipulations to show that sweet- and bitter-sensitivity are independently and reciprocally regulated by starvation in Drosophila. We identify orthogonal neuromodulatory cascades that oppositely control peripheral taste sensitivity for each modality. Moreover, these pathways are recruited at increasing hunger levels, such that low-risk changes (higher sugar sensitivity) precede high-risk changes (lower sensitivity to potentially toxic resources). In this way, state intensity-dependent, reciprocal regulation of appetitive and aversive peripheral gustatory sensitivity permits flexible, adaptive feeding-decisions.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Independent, Reciprocal Neuromodulatory Control of Sweet and Bitter Taste Sensitivity during Starvation in Drosophila

Inagaki

Panse

Anderson

2014

Neuron

167

224

View full text Add to dashboard Cite

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“…The following flies are described elsewhere: S 1 106-GAL4 [13], FB-GAL4 [18], TH-GAL4 [31], Trh-GAL4 [32], c673a-GAL4 [24], Dilp2-GAL4 [33], sNPF-GAL4 [34], AstA-GAL4 [35], and UAS-Shibire ts [36]. …”

Section: Methodsmentioning

confidence: 99%

A fat-derived metabolite regulates a peptidergic feeding circuit in Drosophila

et al. 2017

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Here, we show that the enzymatic cofactor tetrahydrobiopterin (BH4) inhibits feeding in Drosophila. BH4 biosynthesis requires the sequential action of the conserved enzymes Punch, Purple, and Sepiapterin Reductase (Sptr). Although we observe increased feeding upon loss of Punch and Purple in the adult fat body, loss of Sptr must occur in the brain. We found Sptr expression is required in four adult neurons that express neuropeptide F (NPF), the fly homologue of the vertebrate appetite regulator neuropeptide Y (NPY). As expected, feeding flies BH4 rescues the loss of Punch and Purple in the fat body and the loss of Sptr in NPF neurons. Mechanistically, we found BH4 deficiency reduces NPF staining, likely by promoting its release, while excess BH4 increases NPF accumulation without altering its expression. We thus show that, because of its physically distributed biosynthesis, BH4 acts as a fat-derived signal that induces satiety by inhibiting the activity of the NPF neurons.

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“…sNPF is expressed in thousands of neurons in the adult Drosophila brain as well as in endocrine cells of the larval midgut Veenstra, 2009). sNPF has been associated with feeding, growth, and locomotor control (Lee et al, 2004(Lee et al, , 2008(Lee et al, , 2009Kahsai et al, 2010). We used an antiserum directed to a precursor sequence of sNPF to study the sNPF distribution.…”

Section: Peptide Distribution In Relation To Central-complex Markersmentioning

confidence: 99%

Chemical neuroanatomy of the Drosophila central complex: Distribution of multiple neuropeptides in relation to neurotransmitters

Kahsai

Winther

2010

J of Comparative Neurology

100

109

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The central complex of the insect brain is an integration center, receiving inputs from many parts of the brain. In Drosophila it has been associated with the control of both locomotor and visually correlated behaviors. The central complex can be divided into several substructures and is comprised of a large number of neuronal types. These neurons produce classical neurotransmitters, biogenic amines, and different neuropeptides. However, the distribution of neurotransmitters and neuromodulators in central-complex circuits of Drosophila is poorly known. By immunolabeling and GAL4-directed expression of marker proteins, we analyzed the distribution of acetylcholine, glutamate, GABA, monoamines, and eight different neuropeptides; Drosophila tachykinin, short neuropeptide F, myoinhibitory peptide, allatostatin A, proctolin, SIFamide, neuropeptide F, and FMRFamide. All eight neuropeptides were localized to the fan-shaped body, the largest substructure of the central complex, and were mapped to different layers within this structure. Several populations of peptide-immunoreactive tangential and columnar neurons were identified, of which some colocalized acetylcholine. Fewer peptides were found to be expressed in the other substructures: the ellipsoid body, the protocerebral bridge, and the noduli. The ellipsoid body and the protocerebral bridge were innervated by extrinsic peptide expressing neurons. Our findings reveal that numerous neuropeptides are expressed in the central complex and that each peptide has a distinct distribution pattern, suggesting important roles for neuropeptides as neuromediators and cotransmitters in this brain area.

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Processed short neuropeptide F peptides regulate growth through the ERK‐insulin pathway in Drosophila melanogaster

Cited by 30 publications

References 23 publications

Independent, Reciprocal Neuromodulatory Control of Sweet and Bitter Taste Sensitivity during Starvation in Drosophila

Independent, Reciprocal Neuromodulatory Control of Sweet and Bitter Taste Sensitivity during Starvation in Drosophila

A fat-derived metabolite regulates a peptidergic feeding circuit in Drosophila

Chemical neuroanatomy of the Drosophila central complex: Distribution of multiple neuropeptides in relation to neurotransmitters

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