Processed eggshell membrane powder: Bioinspiration for an innovative wound healing product

Ahmed, Tamer A.; Suso, Henri-Pierre; Maqbool, Ayesha; Hincke, Maxwell T.

doi:10.1016/j.msec.2018.10.054

Cited by 70 publications

(62 citation statements)

References 73 publications

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“…The presented data demonstrates the utilization of various extraction strategies (moderate to harsh conditions) [1], [2], [3], [4] to identify the protein constituents of PEP using the proteomic approach (Table 1). A comprehensive PEP proteome was established and compared to the general ESM proteome (Fig.…”

Section: Datamentioning

confidence: 83%

“…Processed eggshell membrane powder (PEP, <100 μm) [3] was subjected to various extraction and solubilization strategies as utilized previously for ESM proteomics [4](Table 1). A complete protein inventory for PEP was created by merging the proteins identified after application of moderate extraction [lithium dodecyl sulphate/dithiothreitol (LDS/DTT) or urea/ammonium bicarbonate (NH 4 HCO3)] and harsh solubilization conditions [3-mercaptopropionic acid (3-MPA) or sodium hydroxide/dimethylsulfoxide (NaOH/DMSO)] (Fig.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…1 and Table 2). Conditions of in-gel (3-MPA, NaOH/DMSO, and LDS/DTT) or in-solution [urea/NH 4 HCO3] tryptic digestion were applied and the resultant peptides were analyzed using the 5600 mass spectrometer with a nanoelectrospray ion source connected to Agilent 1200 nanopump (ES-MS/MS) [3], [4].…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Experimental datasets on processed eggshell membrane powder for wound healing

2019

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Eggshell (ES) and eggshell membrane (ESM) is a significant byproduct of the egg producing industry (Ahmed et al., 2019). Many studies have been undertaken to utilize ES waste for potential value added applications (Cordeiro and Hincke, 2011). Described here are the datasets from our evaluation of processed eggshell membrane powder (PEP) as a wound healing product using the mouse excisional wound splinting model (Ahmed et al., 2019). PEP biomaterial was characterized by proteomics using various extraction and solubilization strategies including moderate (lithium dodecyl sulphate (LDS) and urea/ammonium bicarbonate) and harsh conditions (3-mercaptopropionic acid (3-MPA) and NaOH/dimethylsulfoxide) in order to progressively overcome its stable, insoluble nature (Ahmed et al., 2019, Ahmed et al., 2017). Analysis of proteomic data allowed the relative abundance of the main PEP protein constituents to be determined. The efficacy of PEP for promotion of wound healing was assessed using the mouse excisional wound splinting model, and well-established semi-quantitative histological scoring. (More details about the PEP biomaterial characterization and its in vivo evaluation can be found in the related research article (Ahmed et al., 2019)).

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Section: Datamentioning

confidence: 83%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Experimental datasets on processed eggshell membrane powder for wound healing

2019

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“…It can reduce knee pain and increase flexibility in joint and connective tissue disorder due to the presence of naturally occurring glycosaminoglycans and protein. Various studies also reported that ESM has a useful effect on cutaneous wounds in the early stages of healing . Yang et al 237.…”

Section: Second Generation Agricultural Wastes and Their Usesmentioning

confidence: 99%

Biomass as a sustainable resource for value‐added modern materials: a review

Sharma

Kaur

Punj

et al. 2020

Biofuels Bioprod Bioref

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Over the past century, the world's population has increased greatly. The growth in the population has increased the global food demand, so the food sector has increased its production. As a result of the increased production and consumption, a huge amount of agro-food waste is being generated yearly. Rice husk, wheat straw, sugarcane bagasse, corn husk, peanut shell, eggshell, are just a few examples of agro-food wastes. As these agro-food wastes and their by-products are left unused and unattended, their amount is increasing globally. It is imperative to find ways to put these wastes to environmentally viable use. These wastes find direct uses in biofertilizers, mud houses, animal feed, biofuel, and heat generation in small-scale industries, etc. After heat generation, ashes are considered as a secondary by-product, which can be used as a sustainable and renewable resource for synthesizing value-added products for various applications. This article presents a review of the traditional and advanced methods of utilizing these wastes in household applications, animal feed, fertilizers, fuel generation, pyrolysis, and for the removal of toxic metals from polluted water. These secondary resources can also be used in construction materials and for the synthesis of glasses and glass-ceramics, which can be used in bioengineering, optical, and dielectric materials. Agrofood wastes can be used as resource materials to develop modern materials with better properties instead of using minerals. This not only reduces the environment-and management-related problem of the wastes but also provides a sustainable alternative way to produce cost-effective value-added materials for the benefit of society.

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“…ESM showed an inhibiting effect on bacteria, which favors the long-time storage function of the composite membrane. ESM, as a natural bio-membrane, has wide potential applications in the medical field, such as therapy for burnt skin [27,28], repair of the eardrum, and treatment of rheumatism [29,30]. It would be meaningful to fabricate composite ESM with a high photothermal effect for drug release and medical therapy.…”

Section: Introductionmentioning

confidence: 99%

Sunlight-Driven Photothermal Effect of Composite Eggshell Membrane Coated with Graphene Oxide and Gold Nanoparticles

et al. 2019

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Eggshell membrane (ESM), which consists of unique interwoven shell membrane fibers, provides a unique supporting platform for functional nanoparticles in catalysis and sensing. This work reports a novel strategy for fabricating sunlight-driven photothermal conversion composite membranes by loading graphene oxide (GO) and gold nanoparticles (AuNPs) on the three-dimension (3D) network structured eggshell membrane. Surface morphologies and chemical elements were characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. High photothermal conversion under simulated sunlight irradiation, which may be caused by the synergistic effect of GO and AuNPs, was achieved by coating both GO and AuNPs onto ESM. The temperature of ESM modified with AuNPs, and then GO increased from 26.0 • C to 49.0 • C after 10 min of light irradiation. Furthermore, the nanoscaled GO and AuNPs could add benefit to the heating localization of the obtained composite membrane. It is expected this biocompatible ESM modified with GO and AuNPs would have great potential in drug release and photothermal therapy applications.

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Processed eggshell membrane powder: Bioinspiration for an innovative wound healing product

Cited by 70 publications

References 73 publications

Experimental datasets on processed eggshell membrane powder for wound healing

Experimental datasets on processed eggshell membrane powder for wound healing

Biomass as a sustainable resource for value‐added modern materials: a review

Sunlight-Driven Photothermal Effect of Composite Eggshell Membrane Coated with Graphene Oxide and Gold Nanoparticles

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