Process strategies for enhanced production of 1,3-propanediol by Lactobacillus reuteri using glycerol as a co-substrate

Vieira, Paula Bruzadelle; Kilikian, Beatriz Vahan; Bastos, Rosemary; Perpétuo, Elen Aquino; Nascimento, Cláudio Augusto Oller do

doi:10.1016/j.bej.2014.11.003

Cited by 23 publications

(13 citation statements)

References 40 publications

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“…This pathway is important because it regenerates NAD+ from the NADH that is produced by glucose metabolism and contributes to improved growth [12]. L. reuteri can naturally convert glycerol to 3-HPA, but it cannot directly use 3-HPA as a carbon source for growth [13]. Therefore, the bioconversion of 3-HPA by L. reuteri may be considered a two-step process: (i) proliferation of cells during cultivation and (ii) conversion of glycerol into 3-HPA by resting cells.…”

Section: Introductionmentioning

confidence: 99%

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

Jeon

Lee

et al. 2021

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3-Hydroxypropionaldehyde (3-HPA, reuterin) is a broad-spectrum natural antimicrobial agent used in the food industry and other fields. The low yield from the industrial production of 3-HPA using Lactobacillus reuteri and the spontaneous conversion of 3-HPA to acrolein have limited its more widespread use. We isolated L. reuteri BR201 as a biocatalyst for 3-HPA production and confirmed the effect of each factor in the two-step procedure for 3-HPA bioconversion. After initial cultivation for 8 h (late exponential phase), this isolate produced 378 mM of 3-HPA in 1 h at a concentration of OD600 nm 100, 30 °C, and an initial glycerol concentration of 500 mM. This is the highest reported biocatalytic yield of 3-HPA from a glycerol aqueous solution without additives. We confirmed that 4 mM of 3-HPA had antimicrobial activity against five pathogens. The degradation of 3-HPA to acrolein was greater at high temperatures, and there was little degradation when 3-HPA was maintained at 4 °C for 4 weeks. Our results may be useful for future applications of 3-HPA.

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Section: Introductionmentioning

confidence: 99%

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

Jeon

Lee

et al. 2021

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“…The total amount of consumed glycerol was 67.36 ± 0.53 g/L. The maximum theoretical conversion yield of 1,3-PDO from glycerol is 0.83 g 1,3-PDO per g glycerol (mol-tomol conversion) [38]. Our conversion yield was 0.82 g 1,3-PDO per g glycerol, 98.8% of theoretical maximum.…”

Section: Effect Of Fed-batch Fermentation On Production Of 13-pdomentioning

confidence: 75%

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Wang

Heo

et al. 2020

Microb Cell Fact

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Background: 1,3-propanediol (1,3-PDO) is the most widely studied value-added product that can be produced by feeding glycerol to bacteria, including Lactobacillus sp. However, previous research reported that L. reuteri only produced small amounts and had low productivity of 1,3-PDO. It is urgent to develop procedures that improve the production and productivity of 1,3-PDO. Results:We identified a novel L. reuteri CH53 isolate that efficiently converted glycerol into 1,3-PDO, and performed batch co-fermentation with glycerol and glucose to evaluate its production of 1,3-PDO and other products. We optimized the fermentation conditions and nitrogen sources to increase the productivity. Fed-batch fermentation using corn steep liquor (CSL) as a replacement for beef extract led to 1,3-PDO production (68.32 ± 0.84 g/L) and productivity (1.27 ± 0.02 g/L/h) at optimized conditions (unaerated and 100 rpm). When CSL was used as an alternative nitrogen source, the activity of the vitamin B12-dependent glycerol dehydratase (dhaB) and 1,3-propanediol oxidoreductase (dhaT) increased. Also, the productivity and yield of 1,3-PDO increased as well. These results showed the highest productivity in Lactobacillus species. In addition, hurdle to 1,3-PDO production in this strain were identified via analysis of the half-maximal inhibitory concentration for growth (IC50) of numerous substrates and metabolites. Conclusions:We used CSL as a low-cost nitrogen source to replace beef extract for 1,3-PDO production in L. reuteri CH53. These cells efficiently utilized crude glycerol and CSL to produce 1,3-PDO. This strain has great promise for the production of 1,3-PDO because it is generally recognized as safe (GRAS) and non-pathogenic. Also, this strain has high productivity and high conversion yield.

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“…BAL heterofermentatif, termasuk L. brevis, menggunakan gliserol sebagai penerima elektron dalam kofermentasi dengan glukosa. Adanya gliserol juga mampu menstimulasi pertumbuhan BAL heterofermentatif (Vieira et al, 2015) karena lemak digunakan sebagai sumber energi untuk pertumbuhan bakteri. Kandungan protein merupakan salah satu faktor penting pada susu fermentasi karena berperan dalam pembentukan kekentalan (Delikanli dan Ozcan, 2017).…”

Section: Mutu Kimia Minuman Susu Fermentasiunclassified

Susu Fermentasi Dengan Biji Nangka Sebagai Prebiotik

Rahayu

Suliantari

Safitri

et al. 2020

jtip

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Jackfruit seeds (Artocarpus heterophyllus Lam.) contain dietary fiber, thus it is potential as prebiotic to be used in fermented milk drink. This research aimed to obtain the fermented milk composition made from fresh milk and jackfruit seed flour containing active lactic acid bacteria (LAB), preferred hedonic level and to identify the chemical properties of the resulting fermented milk. Variables of this research were the jackfruit seed flour concentrations of 4, 5, 6% (w/v) and two LAB used (Lactobacillus plantarum and Lactobacillus brevis). The composition was selected based on the viable number of LAB, pH value, and sensory quality. The selected composition was the fermented milk made of fresh milk and 4% (w/v) jackfruit seed flour and L. brevis. The viable number of LAB of the fermented milk was 10.59 log CFU/mL. The sensory quality of the fermented milk was neutral until rather preferred for color, flavor, taste, texture, and overall. The chemical contents (%b/b) of product was 78.16% of moisture content, 2.34% of ash content, 2.85% of fat content, 3.15% of protein content, 13.51% of carbohydrate content, and 1.73% of lactic acid content.

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Process strategies for enhanced production of 1,3-propanediol by Lactobacillus reuteri using glycerol as a co-substrate

Cited by 23 publications

References 40 publications

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Susu Fermentasi Dengan Biji Nangka Sebagai Prebiotik

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