Process-scale disruption of microorganisms

Middelberg, Anton P. J.

doi:10.1016/0734-9750(95)02007-p

Cited by 405 publications

(275 citation statements)

References 147 publications

Supporting

Mentioning

252

Contrasting

Unclassified

Order By: Relevance

“…High-pressure homogenization (HPH) treatment is a mild technology of potential use in food sanitization, due to its capability of preserving the organoleptic and qualitative properties of the fresh product (Popper and Knorr, 1990). When the foodstuff undergoes HPH, the microbial cells experience a non-specific tearing apart of the cell wall determined by the physical interaction of the cells with the small-gap homogenisation valve (Middelberg, 1995). Homogenisation has been extensively used by the dairy and food industry, especially to stabilize food emulsions and to disrupt lipid globules.…”

Section: Application Of High-pressure Homogenisationmentioning

confidence: 99%

High-Pressure Homogenisation for Food Sanitisation

Donsı̀

Ferrari

Maresca

2006

13th World Congress of Food Science &Amp; Technology

View full text Add to dashboard Cite

The inactivation by high-pressure homogenisation of two microbial strains similar in shape but of different membrane resistance, namely a Gram-negative, Escherichia coli, and a Grampositive bacterium, Lactobacillus delbrueckii, was investigated to clarify the main factors influencing cell inactivation. Tests were carried in a lab-scale Stansted Power Fluid highpressure homogenizer up to 250 MPa and several homogenization cycles. Results exhibited a strong dependence on initial cell concentration, which suggests a cell-cell interaction in the disruption valve enhancing bacterial inactivation to higher extent than in other homogenisation apparatus reported in literature. In addition, a strong difference was found between the two bacterial strains, with the Gram-positive one exhibiting a significantly higher resistance to the treatment. Hence, a treatment was optimised for fruit juices, by determining the optimal operating pressure and number of passes through the homogeniser for achieving the lethal disruption of the bacteria, yet preserving the sensory and nutritional attributes.

show abstract

Section: Application Of High-pressure Homogenisationmentioning

confidence: 99%

High-Pressure Homogenisation for Food Sanitisation

Donsı̀

Ferrari

Maresca

2006

13th World Congress of Food Science &Amp; Technology

View full text Add to dashboard Cite

show abstract

“…The cytolysis of fission yeast in pellet (D) was confi rmed in cells treated with HNO 3 at concentration of 36% or above. Various cell disruption strategies have been used to develop an efficient, low-cost, and effective method for release of intracellular products (Middelberg, 1995;Geciova et al, 2002;Ho et al, 2008;Klimek-Ochab et al, 2011;Bzducha-Wróbel et al, 2014). Unfortunately, the method of cell wall digestion reported in previous studies to might be too expensive.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast

et al. 2017

View full text Add to dashboard Cite

-The distribution of metal and metalloid species in each of the cell compartments is termed as "metallome". It is important to elucidate the molecular mechanism underlying the beneficial or toxic effects exerted by a given metal or metalloid on human health. Therefore, we developed a method to measure intracellular metal ion concentration (particularly, intracellular calcium ion) in fission yeast. We evaluated the effects of nitric acid (HNO 3 ), zymolyase, and westase treatment on cytolysis in fission yeast. Moreover, we evaluated the changes in the intracellular calcium ion concentration in fission yeast in response to treatment with/without micafungin. The fission yeast undergoes lysis when treated with 60% HNO 3 , which is simpler and cheaper compared to the other treatments. Additionally, the intracellular calcium ion concentration in 60% HNO 3 -treated fission yeast was determined by inductively coupled plasma atomic emission spectrometry. This study yields significant information pertaining to measurement of the intracellular calcium ion concentration in fission yeast, which is useful for elucidating the physiological or pathological functions of calcium ion in the biological systems. This study is the first step to obtain perspective view on the effect of the metallome in biological systems.

show abstract

“…When this ratio was increased to 1:4, the enzyme activity was decreased which may be due to heat generation during the process resulting in protein denaturation (Middelberg, 1995).…”

Section: Glass Beadsmentioning

confidence: 99%

“…The cell wall of microorganisms provides protection to the cell from osmotic lysis and prevents the release of intracellular content, hence, an efficient cell disruption method is required for the destruction of rigid cell wall mainly peptidoglycan in Gram negative bacteria (Harrison, 1991;Middelberg, 1995).There are various physical, chemical and enzymatic cell disruption methods, but the choice of method is based on the type of microorganism, composition of its cell wall and intracellular location of enzyme (Saptarshi and Lele, 2010). The first step for downstream processing of an intracellular enzyme is the cell disruption and therefore, a feasible cell disruption method with low operating cost and high product recovery should be developed to make it economically viable at large scale.…”

Section: Issn: 2319-7706 Volume 6 Number 8 (2017) Pp 3682-3697mentioning

confidence: 99%