Process parameter studies by central composite design of response surface methodology for lipase activity of newly obtained Actinomycete

Patel, Gayatriben B.; Shah, Kamlesh; Shindhal, Toral; Rakholiya, Parita; Varjani, Sunita

doi:10.1016/j.eti.2021.101724

Cited by 38 publications

(9 citation statements)

References 32 publications

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“…Various substrates possess many properties in them for producing different types of products. Organic compound wastes though toxic to an ecosystem can be used for the production of foodstuffs and other value-added products such as animal feed, biofuels, and biofertilizers (Mishra et al, 2020;Patel et al, 2021). These materials serve as a rich source of minerals, proteins, and sugars.…”

Section: Introductionmentioning

confidence: 99%

Valorization of agro-industrial wastes for biorefinery process and circular bioeconomy: A critical review

Yaashikaa

Kumar

Varjani

2022

Bioresource Technology

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153

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Section: Introductionmentioning

confidence: 99%

Valorization of agro-industrial wastes for biorefinery process and circular bioeconomy: A critical review

Yaashikaa

Kumar

Varjani

2022

Bioresource Technology

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153

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“…A series of p-nitrophenol fatty acid esters of different alkyl carbon chain lengths were used: p-nitrophenyl acetate (p-NPA), C2; p-nitrophenyl butyrate (p-NPB), C4; p-nitrophenyl valerate (p-NPV), C5; p-nitrophenyl caprylate (p-NPC), C8; p-nitrophenol laurate (p-NPL), C12; p-nitrophenol myristate (p-NPM), C14; p-nitrophenyl palmitate (p-NPP) C16; p-nitrophenyl stearate (p-NPS) C18 as substrates and the chain length specificity of the recombinant lipase was assayed at 35°C, in potassium phosphate buffer pH 7.5 according to standard methods. The recombinant lipase enzyme activity was measured and the relative enzyme activity was calculated and the highest enzyme activity was considered as 100% [17].…”

Section: Effects Of Different Substrate On the Lipase Activity Of Rec...mentioning

confidence: 99%

Prokaryotic Expression and Enzymatic Properties of Lipase from Schizochytrium Pombe

Liu¹,

Li²

2023

J Food Tech Nutri Sci

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Based on the codon preference of Escherichia coli, the gene of lipase from Schizochytrium pombe was optimized and the codon adaptation index was 0.8 and the gene was expressed in Escherichia coli as a His-tag fusion protein using pET-30a as the expression vector. The recombinant strain with OD600 about 0.6 was induced with 1 mmol/L isopropyl-β-D-thiogalactoside at 30 °C for 12 h. The purified recombinant lipase STGL3 had a molecular mass of 32 kDa, and the purified lipase had a specific enzyme activity of 2.29 μmol/(min•mg), which was 2.3 times higher than before, and a protein content of 650.00 mg/L. The recombinant STGL3 showed the highest hydrolysis activity at 40 °C and pH 7.5, respectively. The effect of different metal ion concentrations on enzyme activity varied, lipase STGL3 was stable in the presence of Ca2+, while Mg2+, Mn2+, Zn2+, Fe2+ and EDTA had an inhibitory effects on lipase activity. Different types of surfactants and organic solvents inhibited the enzymatic activity of recombinant lipase to different extents. The recombinant lipases showed a preference towards pNP-esters with medium and longer acyl-chains. (C12, C14 and C16) and the recombinant lipases showed relatively weak lipase activity towards the shorter carbon chains. The kinetic constants of lipase were 0.29 mmol/L for Km, 2.28 mmol/(L•min-1 ) for Vmax, and 6.19 S-1 for Kcat. The present study not only achieved the expression and characterization of enzymatic properties of lipase from Leptosphaeria japonica, but also provided some basis for the study of enhancing the quality and quality of algal oil by means of genetic engineering, and further exploring the potential industrial applications of Leptosphaeria japonica lipase.

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“…Rapid human population growth and industrialization have resulted in an increase in energy supply, and source of energy production has not been able to keep up with the rising need (Gaur et al, 2022;Patel et al, 2021). Biomass is an organic substance derived from living organisms that stores energy (mostly carbon).…”

Section: Biomass/feedstock Selectionmentioning

confidence: 99%

Carbon-based catalyst for environmental bioremediation and sustainability: Updates and perspectives on techno-economics and life cycle assessment

Gaur

Gautam

Sharma

et al. 2022

Environmental Research

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Process parameter studies by central composite design of response surface methodology for lipase activity of newly obtained Actinomycete

Cited by 38 publications

References 32 publications

Valorization of agro-industrial wastes for biorefinery process and circular bioeconomy: A critical review

Valorization of agro-industrial wastes for biorefinery process and circular bioeconomy: A critical review

Prokaryotic Expression and Enzymatic Properties of Lipase from Schizochytrium Pombe

Carbon-based catalyst for environmental bioremediation and sustainability: Updates and perspectives on techno-economics and life cycle assessment

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