Process optimization of fucoxanthin production with Tisochrysis lutea

Gao, Fengzheng; Teles, Iago; Wijffels, René H.; Barbosa, María J.

doi:10.1016/j.biortech.2020.123894

Cited by 58 publications

(30 citation statements)

References 37 publications

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“…The Fx reported in literature range between 0.22% and 1.82% for T. lutea , and from 0.19% to 5.92% in P. tricornutum (Gao et al, 2020b; Ishika et al, 2017; Kim et al, 2012a; 2012b; McClure et al, 2018; Mohamadnia et al, 2020; Wu et al, 2016). The results obtained in this work are in accordance to what other authors reported for the same growth conditions, where low P. tricornutum biomass (0.24–0.36 g L −1 ) led to a lower Fx content (0.21%–0.55% DW).…”

Section: Resultsmentioning

confidence: 99%

“…Fx quantification was performed using freeze‐dried biomass according to the method reported previously (Gao et al, 2020b). The biomass, around 2 mg, was extracted four times with 100% ethanol, in bead beating tubes (Lysing Matrix E, 2 ml; MP Biomedicals).…”

Section: Methodsmentioning

confidence: 99%

“…Microalgae are considered sustainable feedstocks for food, feed and chemical ingredients. Several microalgae have been studied for their capacity to accumulate high contents of Fx, such as Tisochrysis lutea and Phaeodactylum tricornutum (Gao et al, 2020b). It is reported in literature that T. lutea can accumulate up to 1.82% DW of Fx (Kim et al, 2012b; Mohamadnia et al, 2020), while P. tricornutum can accumulate as much as 5.92% DW (McClure et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Gao

Sá

Itd

et al. 2020

Biotech & Bioengineering

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The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L−1) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R2 for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L−1: 75.66 and 98.14 mg L−1 d−1, for T. lutea and P. tricornutum, respectively; 0.4 g L−1: 129.9 and 158.47 mg L−1 d−1, T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L−1: 1.14 and 1.41 mg L−1 d−1, T. lutea and P. tricornutum; 0.4 g L−1: 2.09 and 1.73 mg L−1 d−1, T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Gao

Sá

Itd

et al. 2020

Biotech & Bioengineering

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“…performances of those six microalgal strains were compared with neutrophilic C. sorokiniana SAG 211-8K, which is one of the fastest growing microalgae (Cuaresma et al, 2009). Five of the tested strains showed an autotrophic biomass productivity which was 10-39% lower than C. sorokiniana but comparable with other commercially relevant neutrophilic microalgae such as Isocrysis lutea (Gao et al, 2020), Rhodomonas sp. (Oostlander et al, 2020), and Nannochloropsis sp.…”

Section: Discussionmentioning

confidence: 99%

Acid Tolerant and Acidophilic Microalgae: An Underexplored World of Biotechnological Opportunities

et al. 2022

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Despite their large number and diversity, microalgae from only four genera are currently cultivated at large-scale. Three of those share common characteristics: they are cultivated mainly autotrophically and are extremophiles or tolerate “extreme conditions.” Extreme growth conditions aid in preventing contamination and predation of microalgae, therefore facilitating outdoor cultivation. In search for new extremophilic algae suitable for large-scale production, we investigated six microalgal strains able to grow at pH below 3 and belonging to four genera; Stichococcus bacillaris ACUF158, Chlamydomonas acidophila SAG 2045, and Chlamydomonas pitschmannii ACUF238, Viridiella fridericiana ACUF035 and Galdieria sulphuraria ACUF064 and ACUF074. All strains were cultivated autotrophically at light intensity of 100 and 300 μmol m−2 s−1 and pH between 1.9 and 2.9. The autotrophic biomass productivities were compared with one of the most productive microalgae, Chlorella sorokiniana SAG 211-8K, grown at pH 6.8. The acid tolerant strains have their autotrophic biomass productivities reported for the first time. Mixotrophic and heterotrophic properties were investigated when possible. Five of the tested strains displayed autotrophic biomass productivities 10–39% lower than Chlorella sorokiniana but comparable with other commercially relevant neutrophilic microalgae, indicating the potential of these microalgae for autotrophic biomass production under acidic growth conditions. Two acid tolerant species, S. bacillaris and C. acidophila were able to grow mixotrophically with glucose. Chlamydomonas acidophila and the two Galdieria strains were also cultivated heterotrophically with glucose at various temperatures. Chlamydomonas acidophila failed to grow at 37°C, while G. sulphuraria ACUF64 showed a temperature optimum of 37°C and G. sulphuraria ACUF74 of 42°C. For each strain, the biomass yield on glucose decreased when cultivated above their optimal temperature. The possible biotechnological applications of our findings will be addressed.

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“…(Synurophyceae) with the highest known content of fucoxanthin at 26.6 mg/g DW [45]. The highest ever reported fucoxanthin productivity was found in Tisochrysis lutea with 9.81 mg/L/day under batch culture with continuous chemostat dilution [49].…”

Section: Fucoxanthinmentioning

confidence: 97%

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

et al. 2021

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Microalgae are excellent biological factories for high-value products and contain biofunctional carotenoids. Carotenoids are a group of natural pigments with high value in social production and human health. They have been widely used in food additives, pharmaceutics and cosmetics. Astaxanthin, β-carotene and lutein are currently the three carotenoids with the largest market share. Meanwhile, other less studied pigments, such as fucoxanthin and zeaxanthin, also exist in microalgae and have great biofunctional potentials. Since carotenoid accumulation is related to environments and cultivation of microalgae in seawater is a difficult biotechnological problem, the contributions of salt stress on carotenoid accumulation in microalgae need to be revealed for large-scale production. This review comprehensively summarizes the carotenoid biosynthesis and salinity responses of microalgae. Applications of salt stress to induce carotenoid accumulation, potentials of the Internet of Things in microalgae cultivation and future aspects for seawater cultivation are also discussed. As the global market share of carotenoids is still ascending, large-scale, economical and intelligent biotechnologies for carotenoid production play vital roles in the future microalgal economy.

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Process optimization of fucoxanthin production with Tisochrysis lutea

Cited by 58 publications

References 37 publications

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Acid Tolerant and Acidophilic Microalgae: An Underexplored World of Biotechnological Opportunities

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

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