Process Optimization of Enzyme Catalyzed Production of Dietary Diacylglycerol (DAG) Using TLIM as Biocatalyst

Dhara, Rupali

doi:10.5650/jos.ess13159

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…The total carotenoids content of B. rubra seed oil was pretty high (30.5 mg/100 g) compared to sunower seed oil (0.31 mg/100 g). 44 Very high content of total avonoids content seems to be responsible for high free radical scavenging potential of B. rubra seed oil (6.01 mg EC 50 /mL) than sunower seed oil (0.2 mg EC 50 /mL). 52…”

Section: H and 13 C Nmr For Structural Conrmation Of Squalenementioning

confidence: 92%

“…The structure of squalene along with proton and carbon numbering also showed (please see ESI Table 1 † the IV, SV and TAG values of B. rubra seed oil were 79, 92 and 92% of sunower seed oil respectively. 44 It is quite interesting to note that there was 48%, 66%, 77.4% and 60% increase in the FFA, PV, DAG and MAG content respectively in B. rubra seed oil compared to sunower seed oil. The total carotenoids content of B. rubra seed oil was pretty high (30.5 mg/100 g) compared to sunower seed oil (0.31 mg/100 g).…”

Section: H and 13 C Nmr For Structural Conrmation Of Squalenementioning

confidence: 92%

“…The DAG content is 77% higher than the commercial sunower oil reported. 44 Wiesman et al reported that the TAG in vegetable oils is major (95-98%) and the high TAG is vital source of energy and nutrition for humans. 45 Similarly, the DAG is also comparable with the vegetable oil with 6.75% as reported earlier.…”

Section: Physicochemical Composition Of the Oilmentioning

confidence: 99%

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Chemical composition, nutraceuticals characterization, NMR confirmation of squalene and antioxidant activities ofBasella rubraL. seed oil

et al. 2020

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Section: H and 13 C Nmr For Structural Conrmation Of Squalenementioning

confidence: 92%

Section: H and 13 C Nmr For Structural Conrmation Of Squalenementioning

confidence: 92%

See 1 more Smart Citation

Chemical composition, nutraceuticals characterization, NMR confirmation of squalene and antioxidant activities ofBasella rubraL. seed oil

et al. 2020

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“…The separated spots were detected by spraying with 2′,7′‐dichlorofluorescein. Dhara and Singhai () used high‐performance thin‐layer chromatography (HPTLC) to quantify diglycerides of fatty acids in soya oil. The total content of mono‐ and di‐glycerides of fatty acids could be measured by infrared spectroscopy (Murlykina et al., ).…”

Section: Assessmentmentioning

confidence: 99%

Re‐evaluation of mono‐ and di‐glycerides of fatty acids (E 471) as food additives

Younes¹,

Aggett²,

Aguilar³

et al. 2017

EFS2

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The EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS) provides a scientific opinion re-evaluating the safety of mono-and di-glycerides of fatty acids (E 471) when used as a food additive. The Panel considered that it is very likely that hydrolysis of mono-and di-glycerides of fatty acids by lipases in the gastrointestinal tract would occur, resulting in the release of glycerol and fatty acids. Glycerol (E 422) and fatty acids (E 570) have been re-evaluated and the Panel concluded that there was no safety concern regarding their use as food additives. Toxicological studies with mono-and di-glycerides rich in unsaturated fatty acids were considered for the re-evaluation of E 471. No evidence for adverse effects was reported in short-term, subchronic studies, chronic, reproductive and developmental toxicity studies. Neither carcinogenic potential nor a promotion effect in initiation/promotion was reported. The available studies did not raise any concern with regard to genotoxicity. The refined estimates were based on 31 out of 84 food categories in which E 471 is authorised. The Panel noted that the contribution of E 471 represented at the mean only 0.8-3.5% of the recommended daily fat intake. Based on the approach described in the conceptual framework for the risk assessment of certain food additives re-evaluated under Commission Regulation (EU) No 257/2010 and taking into account the considerations mentioned above, the Panel concluded that there was no need for a numerical acceptable daily intake (ADI) and that the food additive mono-and di-glycerides of fatty acids (E 471) was of no safety concern at the reported uses and use levels. The Panel recommended some modifications of the EU specifications for E 471.

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“…Commercial DAG oils are commonly prepared via direct esterification of glycerol with free FAs using immobilized sn ‐1,3‐specific lipases. For the preparation of 1,3‐DAGs, recent studies have used glycerolysis of plant oils (Dhara and Singhal ) as well as direct esterification of glycerol with free FAs (Meng and others ; Sanchez and others ) using Lipozyme RM IM and Lipozyme TL IM. All these published studies on the enzymatic synthesis of MAGs and DAGs employed a batch‐type reaction system.…”

Section: Mags/dagsmentioning

confidence: 99%

Recent Research Trends on the Enzymatic Synthesis of Structured Lipids

Kim

Akoh

2015

Journal of Food Science

134

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Structured lipids (SLs) are lipids that have been chemically or enzymatically modified from their natural biosynthetic form. Because SLs are made to possess desired nutritional, physicochemical, or textural properties for various applications in the food industry, many research activities have been aimed at their commercialization. The production of SLs by enzymatic procedures has a great potential in the future market because of the specificity of lipases and phospholipases used as the biocatalysts. The aim of this review is to provide concise information on the recent research trends on the enzymatic synthesis of SLs of commercial interest, such as medium- and long-chain triacylglycerols, human milk fat substitutes, cocoa butter equivalents, trans-free or low-trans plastic fats (such as margarines and shortenings), low-calorie fats/oils, health-beneficial fatty acid-rich fats/oils, mono- or diacylglycerols, and structurally modified phospholipids. This limited review covers 108 research articles published between 2010 and 2014 which were searched in Web of Science.

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Process Optimization of Enzyme Catalyzed Production of Dietary Diacylglycerol (DAG) Using TLIM as Biocatalyst

Cited by 10 publications

References 23 publications

Chemical composition, nutraceuticals characterization, NMR confirmation of squalene and antioxidant activities ofBasella rubraL. seed oil

Chemical composition, nutraceuticals characterization, NMR confirmation of squalene and antioxidant activities ofBasella rubraL. seed oil

Re‐evaluation of mono‐ and di‐glycerides of fatty acids (E 471) as food additives

Recent Research Trends on the Enzymatic Synthesis of Structured Lipids

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