Process of tight junction recovery in the injured vocal fold epithelium: Morphological and paracellular permeability analysis

Suzuki, Ryo; Katsuno, Tatsuya; Kishimoto, Yo; Nakamura, Ryosuke; Mizuta, Masanobu; Suehiro, Atsushi; Yamashita, Masaru; Nakamura, Tatsuo; Tateya, Ichiro; Omori, Koichi

doi:10.1002/lary.26959

Cited by 10 publications

(13 citation statements)

References 52 publications

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“…34,35 The presence of these proteins in VF tissue has been described in rabbits, 36,37 pigs, 38 and sheep, 39 but the distribution of these proteins has only been localized to the epithelium of rat VFs. 15 Similar to these findings in rats, our study found that ZO-1 and occludin were localized to the rabbit and human epithelium. There was also substantial ZO-1 labeling in the superficial LP of both species, but only substantial occludin labeling in the superficial LP of rabbit samples.…”

Section: Discussionsupporting

confidence: 84%

“…13 Cell-cell adhesion and tight junction molecules such as E-cadherin, ZO-1, and occludin, have been more extensively studied across various animal species and humans, but the localization of this integral cell adhesion protein in animals has not been directly compared to humans. [14][15][16] Although the aforementioned integral membrane proteins have been explored in various animal models, the identification and localization of these proteins has not been localized in rabbit and compared to human VFs. The purpose of the current study was to identify the localization of AQP1, AQP4, AQP7 Na+/K + -ATPase, E-cadherin, ZO-1, and occludin in rabbit and human VFs.…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of the Localization of Integral Membrane Proteins in Human and Rabbit Vocal Folds

et al. 2020

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This study's objective was to identify and compare the localization of Aquaporin (AQP) 1, 4, 7, Na+/K +-ATPase, E-cadherin, zona occludin (ZO)-1, and occludin in human and rabbit vocal folds (VF)s to inform the design of future studies to explore the function of these proteins in the regulation of VF homeostasis. Methods: Four human larynges and five New Zealand white rabbit larynges were used. Samples were immunolabeled for primary antibodies against AQP1, AQP4, AQP7, the alpha subunit of Na+/K +-ATPase, E-cadherin, and ZO-1 and occludin and then captured digitally using a Nikon Eclipse 90i microscope and Hamamatsu C10600 Camera. Two raters familiar with human and rabbit VF histology identified positive labeling in tissue structures, including the apical epithelium, basal epithelium/basement membrane, and lamina propria (LP). Results: Samples from both species showed positive labeling for AQP1 in the basal epithelium/basement membrane, superficial LP, and deep/intermediate LP. Aquaporin 4, Aquaporin 7, Na+/K +-ATPase, and E-cadherin were primarily localized to the epithelium of both species. Zona occludin-1 was primarily localized apical epithelium and the superficial LP of both species. Occludin was primarily present in the apical epithelium in rabbit samples but not human. Conclusion: These data provide evidence of the presence of key ion transport channels and cell adhesion proteins in human and rabbit VFs. Aquaporin 1, 4, 7, Na+/K +-ATPase, E-cadherin, and ZO-1 were similarly localized in both species. These findings will be useful to investigators interested in the exploration of VF homeostasis and barrier integrity in future studies.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

A Comparison of the Localization of Integral Membrane Proteins in Human and Rabbit Vocal Folds

et al. 2020

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“…Interestingly, results of our immunoblotting analysis ( Figure 4 ) showed that MI condition significantly increased the expression of claudin-1, occludin, and zo-1 proteins in the small intestine when compared to normal rats. Claudin-1, occluding and zo-1 belong to the family of proteins that are the most important components of the TJs, where they establish the paracellular barrier that controls the flow of hydrophilic small molecules in the intercellular space between intestinal epithelial cells ( Suzuki et al, 2017 ). Thus, we hypothesized that the poorer permeability through the intestinal epithelial membrane is more likely to be responsible for the decreased values of T max , AUC 0–t , and C max of GA after MI.…”

Section: Resultsmentioning

confidence: 99%

Comparative Pharmacokinetics of Gallic Acid After Oral Administration of Gallic Acid Monohydrate in Normal and Isoproterenol-Induced Myocardial Infarcted Rats

Fan

Jin

et al. 2018

Front. Pharmacol.

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Gallic acid (GA) is a polyphenolic natural product widely distributed in food, beverage, and traditional Chinese herbs with beneficial effects on the cardiovascular system. In this research, a comparative study was conducted to investigate the possible difference of pharmacokinetic process in normal and isoproterenol-induced myocardial infarcted rats after oral administration of GA monohydrate with the dose of 50 and 100 mg/kg, respectively. Quantification of GA in rat plasma was achieved by using a simple and rapid high-performance liquid chromatographic method. The results revealed that pharmacokinetics of GA were greatly different between normal and pathological state. GA exhibited slower absorption into the bloodstream, and yielded 1.7-fold (50 mg/kg GA) and 1.3-fold (100 mg/kg GA) less values of area under concentration–time curve as well as 2.5-fold lower of maximum blood concentration (Cmax) in MI rats than those in normal rats. In addition, significant prolonged T1/2 and MRT as well as decreased CL were also registered in MI rats. Our findings suggest that myocardial infarction could alter the pharmacokinetic process of GA, and thus the potential pharmacokinetic differences of herbal preparations (or dietary nutrition) containing GA between normal and pathological conditions should be brought to the forefront seriously in clinical practice.

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“…This type of vocal fold injury would translate to clinical practice as a type II cordectomy (subligamental cordectomy). The vocal fold stripping model is a well-established first step to address the complex nature of EMC modifications, vocal fold scarring, and fibrosis using various solutions from tissue engineering to stem cells, growth factors, and antifibrotic agents [ 10 – 12 , 15 , 18 , 19 , 23 , 24 ]. Instrumentation tends to be similar, using small gauge needles for the procedure.…”

Section: Discussionmentioning

confidence: 99%

“…Endoscope size ranged from 1.9 mm to 2.7 mm. Endoscope angle was 25–30 degrees in most cases [ 11 , 12 , 14 , 15 , 18 ] and 0 degrees only in 2 cases [ 9 , 16 ]. Proper visualization is essential, and the 25–30s degree, small diameter endoscopes provide the best angle and room for manipulation.…”

Section: Discussionmentioning

confidence: 99%

Vocal fold injury models in rats: a literature review on techniques and methodology

et al. 2022

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This study reviewed the current literature on technical aspects regarding controlled vocal fold injuries in the rat model. Data from PubMed, Embase, and Scopus database for English language literature was collected to identify methodological steps leading to a controlled surgical injury of the rat vocal fold. Inclusion criteria: full disclosure of anesthesia protocol, positioning of the rat for surgery, vocal fold visualization method, instrumentation for vocal fold injury, vocal fold injury type. Articles with partial contribution were evaluated and separately included due to the limited number of original methodologies. 724 articles were screened, and eleven articles were included in the analysis. Anesthesia: ketamine hydrochloride and xylazine hydrochloride varied in dose from 45 mg/kg and 4.5 mg/kg to 100 mg/kg and 10 mg/kg. Visualization: The preferred method was the 1.9 mm, 25–30 degree endoscopes. The widest diameter endoscope used was 2.7 mm with a 0 or 30 degree angle of view. Instruments for lesion induction range from 18 to 31G needles, microscissors, micro forceps to potassium titanyl phosphate, and blue light lasers. Injury types: vocal fold stripping was the main injury type, followed by vocal fold scarring and charring. One article describes scaffold implantation with injury to the superior aspect of the vocal fold. Rats are good candidates for in vivo larynx and vocal folds research. A more standardized approach should be considered regarding the type of vocal fold injury to ease data comparison.

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Process of tight junction recovery in the injured vocal fold epithelium: Morphological and paracellular permeability analysis

Abstract: NA. Laryngoscope, 128:E150-E156, 2018.

Cited by 10 publications

References 52 publications

A Comparison of the Localization of Integral Membrane Proteins in Human and Rabbit Vocal Folds

A Comparison of the Localization of Integral Membrane Proteins in Human and Rabbit Vocal Folds

Comparative Pharmacokinetics of Gallic Acid After Oral Administration of Gallic Acid Monohydrate in Normal and Isoproterenol-Induced Myocardial Infarcted Rats

Vocal fold injury models in rats: a literature review on techniques and methodology

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