Process of Obtaining Bioethanol From Sorghum Biomass Using Genome Shuffling

Abstract

One of the materials to be used for the production of bioethanol might be sorghum, an alternative plant for bioenergy production. In the process of obtaining biofuels, the bioprocess technology is as important as the plant material. The production of bioethanol from lignocellulosic materials requires degrading cell walls to specific polymers and the hydrolysis of carbohydrates to monomer sugars. The aim of the study was to evaluate the chemical and enzymatic treatment of sorghum biomass during the preparation of the material for the production of ethanol. Biomass processing consists in the disintegration of the solid phase and breaking down of compact lignocellulose. The highest content of glucose was obtained using enzymatic hydrolysis with cellulolytic enzyme, i.e. Flashzyme Plus 200. After genome shuffling, the distillery yeast demonstrated an increased fermentation activity and resistance to environmental stress factors formed during the process of ethanol production.

Changes in global DNA methylation under climatic stress in two related grasses suggest a possible role of epigenetics in the ecological success of polyploids

“…However, the present study tested global DNA methylation, without any distinction between CG, CHG and CHH methylation; as such, any conclusions drawn on the differences between the studied species need to be confirmed in further experiments. Furthermore, methylation level can be influenced by numerous other phenomena, such as genome shuffling 25 , genomic shock 26 and subsequent repatterning of expression 27 .…”

“…For F. amethystina , the ploidy level (diploid or tetraploid) of the individuals growing in the same experimental plots was tested using flow cytometry, in accordance with Rewicz et al . 25 (Supplementary Table S1 ).…”

Bioethanol Production From Abies Alba Wood Using Adaptive Neural Fuzzy Interference System Mathematical Modeling

“…In this regard, the autohydrolysis method using only water to extract the hemicellulose fraction as a mixture of oligosaccharides, monosaccharides and secondary by-products can be mentioned. [10][11][12][13][14][15] Separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) are two methods used for the conversion of woody biomass into bioethanol. In SHF, enzymatic hydrolysis and fermentation processes are carried out separately, but these processes have a long reaction time.…”