Process of Destruction of Large Unilamellar Vesicles by a Zwitterionic Detergent, CHAPS: Partition Behavior between Membrane and Water Phases

Viriyaroj, Amornrat; Kashiwagi, Hiroshi; Ueno, Masaki

doi:10.1248/cpb.53.1140

Cited by 12 publications

(11 citation statements)

References 35 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We consider that no available data is inconsistent with this mechanism. This includes the experiments that were considered evidence for both the ''mechanism based on the direct formation of mixed micelles'' (63) and the mechanism based on ''destruction of vesicles via small particles containing large amounts of detergents'' (64).…”

Section: Micellization Of Supersaturated Liposomesmentioning

confidence: 99%

The Mechanism of Detergent Solubilization of Lipid Bilayers

Lichtenberg

Ahyayauch

Goñi

2013

Biophysical Journal

199

245

View full text Add to dashboard Cite

Multiple data are available on the self-assembly of mixtures of bilayer-forming amphiphiles, particularly phospholipids and micelle-forming amphiphiles, commonly denoted detergents. The structure of such mixed assemblies has been thoroughly investigated, described in phase diagrams, and theoretically rationalized in terms of the balance between the large spontaneous curvature of the curvophilic detergent and the curvophobic phospholipids. In this critical review, we discuss the mechanism of this process and try to explain the actual mechanism involved in solubilization. Interestingly, membrane solubilization by some detergents is relatively slow and the common attribute of these detergents is that their trans-bilayer movement, commonly denoted flip-flop, is very slow. Only detergents that can flip into the inner monolayer cause relatively rapid solubilization of detergent-saturated bilayers. This occurs via the following sequence of events: 1), relatively rapid penetration of detergent monomers into the outer monolayer; 2), trans-membrane equilibration of detergent monomers between the two monolayers; 3), saturation of the bilayer by detergents and consequent permeabilization of the membrane; and 4), transition of the whole bilayer to thread-like mixed micelles. When the detergent cannot flip to the inner monolayer, the outer monolayer becomes unstable due to mass imbalance between the monolayers and inclusion of the curvophilic detergent molecules in a flat surface. Consequently, the outer monolayer forms mixed micellar structures within the outer monolayer. Shedding of these micelles into the aqueous solution results in partial solubilization. The consequent leakage of detergent into the liposome results in trans-membrane equilibration of detergent and subsequent micellization through the rapid bilayer-saturation mechanism.

show abstract

Section: Micellization Of Supersaturated Liposomesmentioning

confidence: 99%

The Mechanism of Detergent Solubilization of Lipid Bilayers

Lichtenberg

Ahyayauch

Goñi

2013

Biophysical Journal

199

245

View full text Add to dashboard Cite

show abstract

“…Major deviations from the three-stage model were found for CHAPS (Viriyaroj et al 2005), which shows a binding isotherm with two subsequent transitions indicated by steep increases of X e ( C S aq ) (cf. Fig.…”

Section: Solubilisation Reconstitution Non-lamellar Phasesmentioning

confidence: 99%

Interactions of surfactants with lipid membranes

Heerklotz

2008

Quart. Rev. Biophys.

273

287

View full text Add to dashboard Cite

Surfactants are surface-active, amphiphilic compounds that are water-soluble in the micro- to millimolar range, and self-assemble to form micelles or other aggregates above a critical concentration. This definition comprises synthetic detergents as well as amphiphilic peptides and lipopeptides, bile salts and many other compounds. This paper reviews the biophysics of the interactions of surfactants with membranes of insoluble, naturally occurring lipids. It discusses structural, thermodynamic and kinetic aspects of membrane-water partitioning, changes in membrane properties induced by surfactants, membrane solubilisation to micelles and other phases formed by lipid-surfactant systems. Each section defines and derives key parameters, mentions experimental methods for their measurement and compiles and discusses published data. Additionally, a brief overview is given of surfactant-like effects in biological systems, technical applications of surfactants that involve membrane interactions, and surfactant-based protocols to study biological membranes.

show abstract

“…Hence, composition-dependent transformations of vesicles into micelles (solubilisation) or vice versa (reconstitution) in mixtures of bilayer-forming phospholipids and micelle-forming detergents have received particular attention. The most thoroughly studied detergents include n-octyl-β-D-glucopyranoside (octylglucoside, OG; [2][3][4][5][6]), Triton X-100 [3,7], 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate (CHAPS; [8][9][10]), and other bile acid derivatives [3][4][5]11,12].…”

Section: Introductionmentioning

confidence: 99%