Process Modeling of Enzymatic Hydrolysis of Wet-Exploded Corn Stover

Rana, Vandana; Rana, Diwakar; Ahring, Birgitte Kiær

doi:10.1007/s12155-013-9384-6

Cited by 15 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…where A LA is the amount of levulinic acid obtained (mg/g DW of wood waste), A max LA is the maximum amount of levulinic acid obtainable (mg/g DW of wood waste) assuming that the maximum conversion of hexose to levulinic acid is 64.4% and the amount of hexoses from 1 g of cellulose is 1.111 g [15,51].…”

mentioning

confidence: 99%

Microwave-Assisted One-Step Conversion of Wood Wastes into Levulinic Acid

Kłosowski¹,

Mikulski²,

Menka³

2019

Catalysts

View full text Add to dashboard Cite

This study aimed to evaluate the use of softwood and hardwood waste for the production of levulinic acid by one-stage conversion using microwave radiation combined with acid catalysis. The analysis demonstrated that the type and concentration of the acid used, the concentration of biomass in the reaction mixture and pressure value had the greatest impact on the yield of levulinic acid. The highest efficiency of carbohydrate conversion to levulinic acid, regardless of the type of raw material, was achieved using a pressure of 225 PSI and sulfuric acid as a catalyst. Maximum yield from biomass, ca. 16.5% for cherry wood chips and ca. 25% for pine chips, was obtained using sulfuric acid at a concentration of 1% v/v and 2% v/v, respectively, for the following process parameters: Exposure time 20 min, biomass concentration 3.3%, and the pressure of 225 PSI. The ratio of actual yield to theoretical yield was high: 64.7% ± 4.5% for pine chips and 43.4% ± 1.0% for cherry wood chips. High efficiency of the presented method of biomass conversion to levulinic acid indicates the possibility of its use for waste management in the wood processing industry. High concentration of levulinic acid in the post-reaction mixture allows for cost-effective extraction and purification of the compound.

show abstract

mentioning

confidence: 99%

Microwave-Assisted One-Step Conversion of Wood Wastes into Levulinic Acid

Kłosowski¹,

Mikulski²,

Menka³

2019

Catalysts

View full text Add to dashboard Cite

show abstract

“…The variables were defined in five levels in the following ranges: 3.92-4.70 (R 0 ), 5-15% (TS), and 5-15% (EL). Levels of variables were determined according to preliminary tests and on the basis of literature reports (Cotana et al, 2015;Buratti et al, 2015;Rana et al, 2014;Pereira Ramos et al, 2015).…”

Section: Response Surface Methodology Design (Rsm)mentioning

confidence: 99%

Optimization of the steam explosion and enzymatic hydrolysis for sugars production from oak woods

Cotana¹,

Buratti²,

Barbanera³

et al. 2015

Bioresource Technology

View full text Add to dashboard Cite

“…In studies using corn stover, the highest cellulose hydrolysis yields were reported only at the highest enzyme concentration (40 mg protein per 1 g of glucan) and the highest biomass concentration (20% w/v). At lower enzyme concentrations, a signi cant decrease in hydrolysis yield was observed, even by 35% [33]. Also authors using rice straw as lignocellulosic raw material pointed out that obtaining the highest hydrolysis yield required precise optimization of process parameters.…”

Section: Optimization Of Enzymatic Hydrolysis Of Cellulose In Biomassmentioning

confidence: 99%

“…All analyzes were performed in triplicate. Based on glucose concentration, the yield of cellulose hydrolysis was determined as follows: 1where C Glu is the concentration of glucose (grams per liter) in the sample, C Cel is the cellulose content in biomass (per 1 gram of dry weight), C Biom is the initial biomass content (grams per liter)[33].Assessment of the suitability of microwave hydrotropic extraction in the production of ethanol from maize stillage biomassAlcoholic fermentation was carried out using stillage biomass after microwave hydrotropic extraction under the conditions selected in the rst stage of the study (pressure 117 PSI, extraction time 30 minutes, NaCS concentration 20% v/v, microwave generator power 300 W). The biomass after hydrotropic treatment was subjected to enzymatic hydrolysis using Cellic® CTec2 preparation under the conditions selected in the second stage of the study, ensuring the highest glucose concentration during a 24 h process, i.e.…”

mentioning

confidence: 99%

Microwave-assisted hydrotropic pretreatment as a new and highly efficient way to cellulosic ethanol production from maize distillery stillage

Mikulski

Kłosowski

2020

Preprint

View full text Add to dashboard Cite

Background: The development of the second generation ethanol production technology requires new, highly effective methods of pretreatment of lignocellulosic biomass, which reduces lignin content, eliminates fermentation inhibitors, and makes the biomass susceptible to hydrolysis using cellulolytic enzymes. New pretreatment methods should be adapted to the processing of lignocellulosic waste biomass from various industries. One of the problems is the management of grain stillage, which is waste from the production of first generation ethanol.Results: The aim of the study was to assess the suitability of the combined use of microwave radiation and sodium cumene sulfonate under optimized process conditions for the preparation of maize stillage biomass as a raw material for the production of cellulosic ethanol. The key parameter guaranteeing a high level of lignin removal from biomass (ca. 44%) was the concentration of hydrotrope. The highest organic matter extraction from biomass (67.00±1.68%) was observed for 20% v/v sodium cumene sulfonate, ca. 117 PSI (microwave heating) and 30 min exposure time. Even at high biomass concentration (16% w/v) and a cellulose enzyme dose of about 4 FPU/g, maize stillage biomass subjected to microwave-assisted hydrotropic pretreatment was highly susceptible to enzymatic degradation, which resulted in 80% hydrolysis yield. The stillage biomass processed in this way is a very good raw material for the production of cellulosic ethanol. It is possible to obtain a fermentation medium with a very high glucose concentration (up to 80 g/L), without fermentation inhibitors (such as 5-HMF, furfural, lignin degradation products) and, as a consequence, to reach a very high level of sugar conversion to ethanol, even as much as 95% of theoretical yield.Conclusions: Microwave hydrotropic treatment with sodium cumene sulfonate is a very effective way to prepare waste maize stillage biomass for the production of cellulosic ethanol. The method provides a high level of enzymatic degradation of cellulose, leading to a medium with high content of released sugars suitable for bioconversion, which is in line with the assumptions of the second generation ethanol production technology.

show abstract

Process Modeling of Enzymatic Hydrolysis of Wet-Exploded Corn Stover

Cited by 15 publications

References 17 publications

Microwave-Assisted One-Step Conversion of Wood Wastes into Levulinic Acid

Microwave-Assisted One-Step Conversion of Wood Wastes into Levulinic Acid

Optimization of the steam explosion and enzymatic hydrolysis for sugars production from oak woods

Microwave-assisted hydrotropic pretreatment as a new and highly efficient way to cellulosic ethanol production from maize distillery stillage

Contact Info

Product

Resources

About