Process Evaluation of Sugar-Based Polymeric Colloidal Nanocarrier Formation

Abstract

Several studies have demonstrated that sugar-based nanocarriers are recommended to develop drug delivery systems since they are biocompatible and biodegradable and might improve the therapeutic index of currently used drugs by reducing their toxicity and enhancing their bioavailability. Therewith, this work studied the processing conditions to formation of a sugar-based nanocarrier denominated polymeric colloidal nanocarriers (PCN). It reports the impact of emulsifiers types and concentration, salt concentration and copolymers addition on physical stability and particle size distribution of a maltodextin-based PCN. In order to define the most stable formulation to produce the maltodextrin nanocarriers, 13 PCN formulations were evaluated in respect to kinetic stability, particle size distribution and morphology. The results demonstrated that to have a stable sugar-based PCN in silicone for at least 3 days, it is necessary to work with Poloxamer 188 as a copolymer, SF 1540 surfactant in a concentration proportional to the solid content and a salt concentration of 0.4 %.

Nanomedicine-based strategies to improve treatment of cutaneous leishmaniasis

“…Footpads of mice allow easy measurement of lesion and parasite load [202,205,206]. The base of the tail of rodents and forehead of monkeys are very depicted injection sites [202,207,208]. The ear is used in mice models for inoculation by infected sandflies to simulate natural infection as the needle injections primarily deliver parasites subcutaneously, whereas sandfly introduces the parasite intradermally.…”

“…During the course of in vitro growth, most laboratories harvest the parasite during the late infective stationary phase of growth and employ peanut agglutinin (PNA) to remove non-metacyclic parasites before inoculation. In rodent models, 1 × 10 6 metacyclic-enriched parasites are injected in a volume of 50 µl [205][206][207][208].…”