Process Design and Economics of On-Site Cellulase Production on Various Carbon Sources in a Softwood-Based Ethanol Plant

Barta, Zsolt; Kovács, Krisztina; Réczey, Kati; Zacchi, Guido

doi:10.4061/2010/734182

Cited by 69 publications

(61 citation statements)

References 34 publications

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“…The use of on-site enzyme production in 2G ethanol mills is a feasible way to reduce the costs associated with enzyme purification, addition of stabilizers and preservatives, transportation, and storage. This option should be considered in the design of 2G ethanol bioprocesses [13,14].…”

Section: Introductionmentioning

confidence: 99%

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

Cunha

Vasconcellos

Florencio

et al. 2016

Waste Biomass Valor

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Agro-industrial residues have great potential as low-cost feedstocks for production of hydrolytic enzymes, which play a key role in the economics of cellulosic ethanol production. The goal of this research was to add value to agro-industrial byproducts such as wheat bran (WB), soybean bran, and sugarcane bagasse [either untreated (SCB) or pretreated (SCB p )] by using these materials for on-site production of enzymes employing a non-conventional solid-state and submerged fermentation method. The results were compared to conventional submerged fermentation, and three enzymatic cocktails were selected for use in SCB p hydrolysis. The non-conventional method was successfully validated for enzyme production using all the feedstocks. Compositional analysis and FTIR spectra showed the advantages of using WB, rather than SCB, due to its lower C:N ratio and lower contents of lignin and phenolic derivatives. The use of a WB:SCB mixture resulted in endoglucanase production that was up to 91 % higher than achieved using SCB alone. The best hydrolysis performance was achieved with the WB enzymatic cocktail, and the mixture of WB:SCB p resulted in cocktails with performance 51 % higher than cocktails produced with SCB p alone. The non-conventional on-site enzyme production using agro-industrial byproducts could potentially contribute to the implementation of large-scale 2G ethanol plants.

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Section: Introductionmentioning

confidence: 99%

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

Cunha

Vasconcellos

Florencio

et al. 2016

Waste Biomass Valor

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“…In the liquid fraction of the slurry the yeast is cultivated and then supplemented with molasses. SSF is performed at 37 ºC with enzymes and yeast (Barta et al, 2010a;Sassner et al, 2008). The configuration of the process after SSF depends on the various combinations of the desired by-products.…”

Section: Biofuel Production Through Polygenerationmentioning

confidence: 99%

Introduction of large-scale biofuel production in a district heating system – an opportunity for reduction of global greenhouse gas emissions

Ilić

Dotzauer

Trygg

et al. 2014

Journal of Cleaner Production

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In this study, cooperation between Stockholm's transport and district heating sectors is sectors by between 20 % and 65 %, depending on energy market conditions and assumed transport sector scenarios. If we consider biomass an unlimited resource, the potential for greenhouse gas emissions reduction is significant. However, considering that biomass is a limited resource, the increase of biomass use in the district heating system may lead to a decrease of biomass use in other energy systems. The potential for reduction of global greenhouse gas emissions is thus highly dependent on the alternative use of biomass. If this alternative is used for co-firing in coal condensing power plants, biomass use in combined heat and power plants would be more desirable than biofuel production through polygeneration. On the other hand, if this alternative is used for traditional biofuel production (without co-production of heat and electricity), the benefits of biofuel production through polygeneration from a greenhouse gas emissions perspective is superior. However, if carbon capture and storage technology is applied on the biofuel polygeneration plants, the introduction of large-scale biofuel production into the district heating system would result in a reduction of global greenhouse gas emissions independent of the assumed alternative use of biomass. AbbreviationsDH, district heating; TS, transport sector; DHS, district heating system; GHG, greenhouse gas; FT, Fischer-Tropsch; DME, dimethyl ether; SSF, simultaneous saccharification and fermentation; CHP, combined heat and power; PHEV, Plug-in Hybrid Electric Vehicles; E10, 90 % gasoline mixed with 10 % ethanol; MODEST, Model for Optimisation of Dynamic Energy Systems with Time-dependent components 3 and boundary conditions; EMS, energy market scenario; CO 2 , carbon dioxide; gGHGes, global GHG emissions of the studied system; CCS, carbon capture and storage.

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“…A techno-economic evaluation of the spruce-toethanol process, based on SO 2 -catalysed steam pretreatment followed by simultaneous saccharification and fermentation with various process configurations, achieved an ethanol cost of about 0.38-0.50 €/L. Anaerobic digestion of the stillage with biogas upgrading was a demonstrably favorable option in terms of both energy efficiency and ethanol production cost 8,9 and the contribution of enzyme is reported to be 0.04-0.05 €/L 9 . Ballerini et al 5 concluded that technical and economic optimization of the pretreatment step, the total substitution of lactose by pentose hydrolysate as the main carbon source for enzyme production, and the recycling of a fraction of the enzyme, the incorporation of pentose in ethanol fermentation, and the utilization of by-products all reduce the production cost of lignocellulosic ethanol.…”

Section: Cost Analysismentioning

confidence: 99%

A Review of Life Cycle Assessment (LCA) of Bioethanol from Lignocellulosic Biomass

Roy

Tokuyasu

Orikasa

et al. 2012

JARQ

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Liquid biofuels are widely recognized alternatives to fossil fuel not only to combat the global warming potential, but also to reduce the reliance on fossil fuels to facilitate economic development. The production and use of lignocellulosic liquid biofuel have been emphasized because it is highly reproducible and does not compete with food. This study summarizes the LCA studies on lignocellulosic ethanol produced from various biomass resources focusing on energy balance, greenhouse gas (GHG) emission and other impact categories, and the production cost to discuss their potential environmental and socioeconomic impacts. Numerous efforts have been made to evaluate the life cycle of lignocellulosic ethanol with LCA methodologies and deals with feedstock, energy paths, conversion technologies, allocation methods, utilization of by-products etc. to determine the environmental impacts as well as the production cost. The environmental benefits are reported in most of the studies except for few examples. A wide variation was observed in the reported production cost of ethanol, which is dependent on the feedstock, conversion technologies, allocation methods and plant sizes. Onsite enzymes production/purchase appeared to be the main hotspot, demands a vigorous study to improve their productivity and reduce costs. Another promising alternative for compensating production costs seem to be the generation of valuable coproducts and integration of ethanol production processes (ethanol and energy). Reviewed literature indicates that despite the environmental benefits of ethanol produced from lignocellulosic biomass, its economic viability remains doubtful at present, even if highly optimistic assumptions are made for the cost calculation, especially in the case of enzyme. Hence, the biotechnological revolution is must for the sustainability of bioethanol, especially in the field of enzymes and microorganisms. Moreover, the adaptation of innovative technologies and renewable energy policy may help limit costs, but careful consideration of land use changes and soil quality is required to avoid any loss of productivity.

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Process Design and Economics of On-Site Cellulase Production on Various Carbon Sources in a Softwood-Based Ethanol Plant

Cited by 69 publications

References 34 publications

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

On-Site Production of Enzymatic Cocktails Using a Non-conventional Fermentation Method with Agro-Industrial Residues as Renewable Feedstocks

Introduction of large-scale biofuel production in a district heating system – an opportunity for reduction of global greenhouse gas emissions

A Review of Life Cycle Assessment (LCA) of Bioethanol from Lignocellulosic Biomass

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