Process and environmental simulation in the validation of the biotechnological production of nisin from waste

Emulsifying salts and P2O5 content as promising strategies to control Bacillus cereus growth during storage of processed cheese

“…The use of emulsifying salts with antimicrobial effect can be a viable strategy for PC, offering an alternative to replace antimicrobial additives such as nisin and potassium sorbate. While nisin is recognised as an effective antimicrobial, its production costs remain high (Arias et al 2021). In a previous study, we compared the inhibitory effects of emulsifying salts and nisin + potassium sorbate in PC.…”

“…While nisin is recognised as an effective antimicrobial, its production costs remain high (Arias et al . 2021). In a previous study, we compared the inhibitory effects of emulsifying salts and nisin + potassium sorbate in PC.…”

Macroalgae biorefineries as a sustainable resource in the extraction of value-added compounds

“…As for cell disruption alternatives, the most commonly used are freeze thawing [8], sonication and/or enzymatic hydrolysis with lysozyme. Some of the drawbacks of the freezing process are the time required for cell lysis and the required operating temperatures: − 20 • C [9], which will significantly affect the energy requirements of these steps and the associated environmental impacts [10]. However, the amount of phycoerythrin extracted by freeze-drying achieves a significantly higher value but also a higher purity [11].…”

Modelling and Environmental Profile Associated with the Valorization of Wheat Straw as Carbon Source in the Biotechnological Production of Manganese Peroxidase

“…Although there is a wide range of ligninolytic fungal species, Irpex lacteus is considered a platform for the overexpression of oxidative enzymes: laccase and MnP from lignocellulosic waste streams, thus emerging as an alternative with potential for large-scale development [35,36]. However, research efforts should also focus on achieving high levels of enzyme activity, reducing production costs as well as the impacts associated with their production processes [37][38][39].…”

“…For this purpose, it is necessary to apply process modelling tools to determine the technical feasibility of the conceptual design and thus compile the necessary data to carry out the environmental assessment. The Life Cycle Assessment (LCA) methodology is considered a versatile methodology to quantify the damage potential and environmental burdens of the process and/or product(s) and to identify the main hotspots or those process steps that contribute most on the environmental profile, on which future research should be focused to increase process efficiency and enable a more sustainable process route [39][40][41][42]. Thus, the results of this assessment provide valuable information on the technological and environmental feasibility of MnP production on an industrial scale.…”