Proceedings of the 2010 National Toxicology Program Satellite Symposium

Adams, E. Terence; Auerbach, Sanford; Blackshear, Pamela E.; Bradley, Alys; Gruebbel, Margarita M.; Little, P B; Malarkey, David E.; Maronpot, Robert R.; McKay, Jennifer S.; Miller, Rodney A.; Moore, Rebecca R.; Morrison, James P.; Nyska, Abraham; Ramot, Yuval; Rao, Deepa B.; Suttie, Andrew W.; Wells, Monique Y.; Willson, Gabrielle A.; Elmore, Susan A.

doi:10.1177/0192623310391680

Cited by 21 publications

(40 citation statements)

References 51 publications

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“…In the moderately and markedly affected mice, the normal glomerular structure was severely distorted, commonly with obliteration of capillary lumens and a lack of conspicuous normal glomerular features, although the basement membrane (BM) could typically be distinguished. The deposits were generally well-circumscribed, variably-sized though often extensive, and composed of moderately electron dense curvilinear, non-branching, closely packed fibrils often organized into characteristic whorls or “fingerprint” patterns (Figures 9C–9F) as previously described (Adams et al 2011; Frazier et al 2014; Wojcinski et al 1991). The fibrils were, on average, approximately 15 nm in diameter with an average intra-fibril distance of 25 nm.…”

Section: Resultssupporting

confidence: 67%

Comparison of Renal Amyloid and Hyaline Glomerulopathy in B6C3F1 Mice

et al. 2016

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Due to potential misdiagnosis of hyaline glomerulopathy (HG) for amyloidosis, a retrospective study of B6C3F1 mice from the National Toxicology Program (NTP) archives was undertaken to determine if HG had occurred in prior NTP studies and, if so, if these two glomerular lesions could be routinely discriminated. Kidney slides from 7 amyloid positive control mice, 2 HG positive control mice, 3 normal or negative control mice, and 41 potential HG mice (with renal-only deposits previously diagnosed as amyloid) were evaluated using hematoxylin and eosin, periodic acid Schiff (PAS), Congo red (CR) and Masson’s trichrome (MT) stains. Utilizing these techniques, HG was reliably distinguished from amyloidosis. All 41 potential HG mice had glomerular deposits histochemically inconsistent with amyloid; the deposits were PAS positive and CR negative. Four of the 41 mice were selected for transmission electron microscopy of the glomerular deposits; ultrastructurally the deposits in these animals were consistent with HG and not amyloid. Our findings indicate that HG is a spontaneous lesion in B6C3F1 mice of low occurrence, is commonly misdiagnosed as amyloidosis, and is more likely than amyloid to cause glomerular deposits in mice without evidence of deposits in other tissues. Also, HG can be distinguished from amyloid on H&E evaluation; however, the distinction is improved with use of PAS or CR staining, and/or ultraviolet evaluation.

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Section: Resultssupporting

confidence: 67%

Comparison of Renal Amyloid and Hyaline Glomerulopathy in B6C3F1 Mice

et al. 2016

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“…All 3 ependymomas were designed as malignant based on intratumoral necrosis and/or invasion of the surrounding neuroparenchyma. 1,25 Immunohistochemical examination confirmed lack of glial fibrillary acidic protein expression and diffuse vimentin positivity, features characteristic for rodent ependymoma. 1 In addition, individual tumor cells displayed unequivocal cytoplasmic wide-spectrum cytokeratin immunoreactivity, as seen in ependymal tumors of humans, cats, and dogs.…”

Section: Discussionmentioning

confidence: 80%

“…1,25 Immunohistochemical examination confirmed lack of glial fibrillary acidic protein expression and diffuse vimentin positivity, features characteristic for rodent ependymoma. 1 In addition, individual tumor cells displayed unequivocal cytoplasmic wide-spectrum cytokeratin immunoreactivity, as seen in ependymal tumors of humans, cats, and dogs. 35,55,69,74 In our study, an accurate comparison of tumor frequencies between sexes is limited by the smaller female cohort.…”

Section: Discussionmentioning

confidence: 80%

“…1,25 A recent retrospective database analysis identified 3 cases of ependymoma on >100 000 mice surveyed. 1 In view of these data, the prevalence of ependymomas observed in our cohort is unexpectedly high. Analysis of a larger number of aging animals may clarify whether a real predisposition for ependymal neoplasms exists in the 129S6/SvEvTac substrain.…”

Section: Discussionmentioning

confidence: 99%

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The Pathology of Aging 129S6/SvEvTac Mice

et al. 2015

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The 129 mouse strain is commonly used for the generation of genetically engineered mice. Genetic drift or accidental contamination during outcrossing has resulted in several 129 substrains. Comprehensive data on spontaneous age-related pathology exist for the 129S4/SvJae substrain, whereas only limited information is available for other 129 substrains. This longitudinal aging study describes the life span and spontaneous lesions of 44 male and 18 female mice of the 129S6/SvEvTac substrain. Median survival time was 778 and 770 days for males and females, respectively. Tumors of lung and Harderian gland were the most common neoplasms in both sexes. Hepatocellular tumors occurred mainly in males. Hematopoietic tumors were observed at low frequency. Suppurative and ulcerative blepharoconjunctivitis was the most common nonneoplastic condition in both sexes. Corynebacteria (primarily Corynebacterium urealyticum and C. pseudodiphtheriticum) were isolated from animals with blepharoconjunctivitis and in some cases from unaffected mice, although a clear causal association between corynebacterial infections and blepharoconjunctivitis could not be inferred. Polyarteritis occurred only in males and was identified as the most common nonneoplastic contributory cause of death. Eosinophilic crystalline pneumonia occurred in both sexes and was a relevant cause of death or comorbidity. Epithelial hyalinosis at extrapulmonary sites was noted at higher frequency in females. This study contributes important data on the spontaneous age-related pathology of the 129S6/SvEvTac mouse substrain and is a valuable reference for evaluation of the phenotype in genetically engineered mice obtained with this 129 substrain.Keywords 129 mouse, aging, blepharoconjunctivitis, Harderian gland, hyalinosis, phenotyping Each inbred laboratory mouse strain exhibits a unique spectrum of naturally occurring lesions that develop progressively with age. Crosses of different strains usually result in variations of the original strain-specific phenotype, which include vanishing, attenuation, or exacerbation of expected lesions or even development of completely new conditions. 20,72 In this context, an accurate definition of strain-specific pathology in those inbred mice that are most frequently used in biomedical research (especially for the generation of genetically engineered mice) is crucial to understand whether a phenotype results from the experimental intervention or rather reflects a naturally occurring entity. 3,54,62 In addition, longitudinal survival studies determining the aging phenotype of inbred strains are particularly important, as they provide a useful reference that enables the selection of the most appropriate genetic background for specific experiments and facilitates the interpretation of clinicopathologic changes that develop in long-term studies. 3,45,72 Thorough and comprehensive pathologic analysis of inbred strains is important not only for interpreting lesions in the setting of hypothesis-driven research but also for large-sc...

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“…The National Toxicology Program (NTP) Satellite Symposium is a 1-day meeting that is traditionally held in conjunction with the annual Society of Toxicologic Pathology (STP) meeting (Bach et al 2010; Adams et al 2011). Attendance at the symposium has steadily increased since the first meeting in 2000, and this year there were 260 registered participants.…”

Section: Introductionmentioning

confidence: 99%

Proceedings of the 2012 National Toxicology Program Satellite Symposium

et al. 2012

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The 2012 annual National Toxicology Program (NTP) Satellite Symposium, entitled “Pathology Potpourri,” was held in Boston in advance of the Society of Toxicologic Pathology’s 31st annual meeting. The goal of the NTP Symposium is to present current diagnostic pathology or nomenclature issues to the toxicologic pathology community. This article presents summaries of the speakers’ presentations, including diagnostic or nomenclature issues that were presented, along with select images that were used for audience voting or discussion. Some lesions and topics covered during the symposium include eosinophilic crystalline pneumonia in a transgenic mouse model; differentiating adrenal cortical cystic degeneration from adenoma; atypical eosinophilic foci of altered hepatocytes; differentiating cardiac schwannoma from cardiomyopathy; diagnosis of cardiac papillary muscle lesions; intrahepatocytic erythrocytes and venous subendothelial hepatocytes; lesions in Rathke’s cleft and pars distalis; pernicious anemia and megaloblastic disorders; embryonic neuroepithelial dysplasia, holoprosencephaly and exencephaly; and INHAND nomenclature for select cardiovascular lesions.

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Proceedings of the 2010 National Toxicology Program Satellite Symposium

Cited by 21 publications

References 51 publications

Comparison of Renal Amyloid and Hyaline Glomerulopathy in B6C3F1 Mice

Comparison of Renal Amyloid and Hyaline Glomerulopathy in B6C3F1 Mice

The Pathology of Aging 129S6/SvEvTac Mice

Proceedings of the 2012 National Toxicology Program Satellite Symposium

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